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AB56636

Recombinant human MAP4K5 protein (GST tag N-Terminus)

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Recombinant human MAP4K5 protein (GST tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, FuncS.
4 Images
Functional Studies - Recombinant human MAP4K5 protein (GST tag N-Terminus) (AB56636)
  • FuncS

Unknown

Functional Studies - Recombinant human MAP4K5 protein (GST tag N-Terminus) (AB56636)

The specific activity of MAP4K5 (ab56636) was determined to be 75 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human MAP4K5 protein (GST tag N-Terminus) (AB56636)
  • FuncS

Unknown

Functional Studies - Recombinant human MAP4K5 protein (GST tag N-Terminus) (AB56636)

Kinase Assay using Myelin Base protein as the substrate. Specific Activity : 86 nmol/min/mg.

SDS-PAGE - Recombinant human MAP4K5 protein (GST tag N-Terminus) (AB56636)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAP4K5 protein (GST tag N-Terminus) (AB56636)

SDS Page analysis of ab56636

SDS-PAGE - Recombinant human MAP4K5 protein (GST tag N-Terminus) (AB56636)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAP4K5 protein (GST tag N-Terminus) (AB56636)

SDS PAGE analysis of ab56636

Key facts

Purity

>75% Densitometry

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity: 86 nm/min/mg.

Accession

Q9Y4K4-1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Kinase assay.</p>" } } }

Product details

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity.

Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9Y4K4","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True

General info

Function

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Product protocols

Target data

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.
See full target information MAP4K5

Alternative Names

Mitogen-activated protein kinase kinase kinase kinase 5, Kinase homologous to SPS1/STE20, MAPK/ERK kinase kinase kinase 5, KHS, MEK kinase kinase 5, MEKKK 5, MAP4K5

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