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AB55712

Recombinant human MAP4K6 protein

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Recombinant human MAP4K6 protein is a Human Fragment protein, in the 1 to 320 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

B55, MAP4K6, MINK, YSK2, ZC3, MINK1, Misshapen-like kinase 1, GCK family kinase MiNK, MAPK/ERK kinase kinase kinase 6, Misshapen/NIK-related kinase, Mitogen-activated protein kinase kinase kinase kinase 6, MEK kinase kinase 6, MEKKK 6

4 Images
Functional Studies - Recombinant human MAP4K6 protein (AB55712)
  • FuncS

Unknown

Functional Studies - Recombinant human MAP4K6 protein (AB55712)

The specific activity of MAP4K6 (ab55712) was determined to be 22 nmol/min/mg as per activity assay protocol and was equivalent to 22 nmol/min/mg as per radiometric assay

SDS-PAGE - Recombinant human MAP4K6 protein (AB55712)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAP4K6 protein (AB55712)

SDS PAGE analysis of ab55712

SDS-PAGE - Recombinant human MAP4K6 protein (AB55712)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MAP4K6 protein (AB55712)

SDS-PAGE analysis of ab55712 with molecular weight markers. Approximate molecular weight 61kDa.

Functional Studies - Recombinant human MAP4K6 protein (AB55712)
  • FuncS

Unknown

Functional Studies - Recombinant human MAP4K6 protein (AB55712)

Kinase assay using ab55712. Specific activity : 19 nmol/min/mg.

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: 19 nmol/min/mg

Accession

Q8N4C8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity

Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":320,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q8N4C8","tags":[]}]

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking (PubMed : 10708748, PubMed : 16337592). Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons (By similarity). Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates TANC1 upon stimulation by RAP2A, MBP and SMAD1 (PubMed : 18930710, PubMed : 21690388). Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1 (By similarity). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed : 26437443).. Isoform 4 can activate the JNK pathway. Involved in the regulation of actin cytoskeleton reorganization, cell-matrix adhesion, cell-cell adhesion and cell migration.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Post-translational modifications

Autophosphorylated.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking (PubMed : 10708748, PubMed : 16337592). Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons (By similarity). Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates TANC1 upon stimulation by RAP2A, MBP and SMAD1 (PubMed : 18930710, PubMed : 21690388). Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1 (By similarity). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed : 26437443).. Isoform 4 can activate the JNK pathway. Involved in the regulation of actin cytoskeleton reorganization, cell-matrix adhesion, cell-cell adhesion and cell migration.
See full target information MINK1

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