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Recombinant Human MAPRE1/EB1 protein is a Human Full Length protein, in the 2 to 268 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Alternative names=Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, MAPRE1
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Sequence
M A S M T G G Q Q M G R G H H H H H H E N L Y F Q G G E F A V N V Y S T S V T S D N L S R H D M L A W I N E S L Q L N L T K I E Q L C S G A A Y C Q F M D M L F P G S I A L K K V K F Q A K L E H E Y I Q N F K I L Q A G F K R M G V D K I I P V D K L V K G K F Q D N F E F V Q W F K K F F D A N Y D G K D Y D P V A A R Q G Q E T A V A P S L V A P A L N K P K K P L T S S S A A P Q R P I S T Q R T A A A P K A G P G V V R K N P G V G N G D D E A A E L M Q Q V N V L K L T V E D L E K E R D F Y F G K L R N I E L I C Q E N E G E N D P V L Q R I V D I L Y A T D E G F V I P D E G G P Q E E Q E E Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Recommended products
Recombinant Human MAPRE1/EB1 protein is a Human Full Length protein, in the 2 to 268 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Alternative names=Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, MAPRE1
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Predicted molecular weight
33.2 kDa
- Amino acids
2 to 268
- Sequence
M A S M T G G Q Q M G R G H H H H H H E N L Y F Q G G E F A V N V Y S T S V T S D N L S R H D M L A W I N E S L Q L N L T K I E Q L C S G A A Y C Q F M D M L F P G S I A L K K V K F Q A K L E H E Y I Q N F K I L Q A G F K R M G V D K I I P V D K L V K G K F Q D N F E F V Q W F K K F F D A N Y D G K D Y D P V A A R Q G Q E T A V A P S L V A P A L N K P K K P L T S S S A A P Q R P I S T Q R T A A A P K A G P G V V R K N P G V G N G D D E A A E L M Q Q V N V L K L T V E D L E K E R D F Y F G K L R N I E L I C Q E N E G E N D P V L Q R I V D I L Y A T D E G F V I P D E G G P Q E E Q E E Y
- Accession
- Q15691-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.32% Tris HCl
Specifications
- Form
Liquid
- Additional notes
ab182821 was expressed in E.coli as inclusion bodies, refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.
General info
- Function
Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570). Promotes cytoplasmic microtubule nucleation and elongation (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570). May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes (PubMed:12388762). Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570). Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed:28726242). May play a role in cell migration (By similarity).
- Sequence similarities
Belongs to the MAPRE family.
- Subcellular localisation
Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-80°C
Notes
This product was previously labelled as MAPRE1
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