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AB314524

Recombinant human MARCH2 protein (His tag)

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Recombinant human MARCH2 protein (His tag) is a Human Full Length protein, in the 1 to 246 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

MARCH2, RNF172, HSPC240, MARCHF2, E3 ubiquitin-protein ligase MARCHF2, Membrane-associated RING finger protein 2, Membrane-associated RING-CH protein II, RING finger protein 172, RING-type E3 ubiquitin transferase MARCHF2, MARCH-II

1 Images
SDS-PAGE - Recombinant human MARCH2 protein (His tag) (AB314524)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human MARCH2 protein (His tag) (AB314524)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. The difference between the theoretical value and the observed value may be related to the molecular structure of the protein. It is predicted that the isoelectric point pI of the fusion protein is 7.46, the acidic protein usually shows higher molecular weight when runs on the gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9P0N8

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

pH: 7.4 - 8 Constituents: PBS, 6% Trehalose

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
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Sequence info

[{"sequence":"MTTGDCCHLPGSLCDCSGSPAFSKVVEATGLGPPQYVAQVTSRDGRLLSTVIRALDTPSDGPFCRICHEGANGECLLSPCGCTGTLGAVHKSCLEKWLSSSNTSYCELCHTEFAVEKRPRPLTEWLKDPGPRTEKRTLCCDMVCFLFITPLAAISGWLCLRGAQDHLRLHSQLEAVGLIALTIALFTIYVLWTLVSFRYHCQLYSEWRKTNQKVRLKIREADSPEGPQHSPLAAGLLKKVAEETPV","proteinLength":"Full Length","predictedMolecularWeight":"43 kDa","actualMolecularWeight":null,"aminoAcidEnd":246,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9P0N8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C|-80°C
Appropriate long-term storage conditions
-20°C|-80°C
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Lyophilized

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General info

Function

E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (PubMed : 14722266, PubMed : 16428329). Together with GOPC/CAL mediates the ubiquitination and lysosomal degradation of CFTR (PubMed : 23818989). Ubiquitinates and therefore mediates the degradation of DLG1 (PubMed : 17980554). Regulates the intracellular trafficking and secretion of alpha1-antitrypsin/SERPINA1 and HP/haptoglobin via ubiquitination and degradation of the cargo receptor ERGIC3 (PubMed : 31142615). Negatively regulates the antiviral and antibacterial immune response by repression of the NF-kB and type 1 IFN signaling pathways, via MARCHF2-mediated K48-linked polyubiquitination of IKBKG/NEMO, resulting in its proteasomal degradation (PubMed : 32935379). May be involved in endosomal trafficking through interaction with STX6 (PubMed : 15689499).. (Microbial infection) Positively regulates the degradation of Vesicular stomatitis virus (VSV) G protein via the lysosomal degradation pathway (PubMed : 29573664). Represses HIV-1 viral production and may inhibit the translocation of HIV-1 env to the cell surface, resulting in decreased viral cell-cell transmission (PubMed : 29573664).

Subcellular localisation

Lysosome membrane

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Product protocols

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Target data

E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (PubMed : 14722266, PubMed : 16428329). Together with GOPC/CAL mediates the ubiquitination and lysosomal degradation of CFTR (PubMed : 23818989). Ubiquitinates and therefore mediates the degradation of DLG1 (PubMed : 17980554). Regulates the intracellular trafficking and secretion of alpha1-antitrypsin/SERPINA1 and HP/haptoglobin via ubiquitination and degradation of the cargo receptor ERGIC3 (PubMed : 31142615). Negatively regulates the antiviral and antibacterial immune response by repression of the NF-kB and type 1 IFN signaling pathways, via MARCHF2-mediated K48-linked polyubiquitination of IKBKG/NEMO, resulting in its proteasomal degradation (PubMed : 32935379). May be involved in endosomal trafficking through interaction with STX6 (PubMed : 15689499).. (Microbial infection) Positively regulates the degradation of Vesicular stomatitis virus (VSV) G protein via the lysosomal degradation pathway (PubMed : 29573664). Represses HIV-1 viral production and may inhibit the translocation of HIV-1 env to the cell surface, resulting in decreased viral cell-cell transmission (PubMed : 29573664).
See full target information MARCHF2
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