Recombinant human MARK4 protein
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(1 Publication)
Recombinant human MARK4 protein is a Human Full Length protein, in the 1 to 752 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.
View Alternative Names
KIAA1860, MARKL1, MARK4, MAP/microtubule affinity-regulating kinase 4, MAP/microtubule affinity-regulating kinase-like 1
- FuncS
Unknown
Functional Studies - Recombinant human MARK4 protein (AB105211)
The Specific activity of ab105211 was determined to be 1050 nmol/min/mg.
- FuncS
Unknown
Functional Studies - Recombinant human MARK4 protein (AB105211)
The specific activity of MARK4 (ab105211) was determined to be 1140 nmol/min/mg as per activity assay protocol
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human MARK4 protein (AB105211)
SDS-PAGE showing ab105211 at approximately 104kDa.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human MARK4 protein (AB105211)
SDS PAGE analysis of ab105211
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
Additional notes
Purity was determined to be >90% by densitometry. Affinity purified.
General info
Function
Serine/threonine-protein kinase (PubMed : 14594945, PubMed : 15009667, PubMed : 23184942, PubMed : 23666762). Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed : 14594945, PubMed : 23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed : 14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed : 14594945, PubMed : 25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed : 23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed : 23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed : 25123532). Reduces neuronal cell survival (PubMed : 15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed : 23184942). Involved in NLRP3 positioning along microtubules by mediating NLRP3 recruitment to microtubule organizing center (MTOC) upon inflammasome activation (PubMed : 28656979).
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Post-translational modifications
Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.. Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).
Subcellular localisation
Cytoskeleton
Target data
Publications (1)
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Acta neuropathologica communications 7:135 PubMed31429800
2019
Applications
Unspecified application
Species
Unspecified reactive species
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