Recombinant Human MASA protein is a Human Full Length protein, in the 1 to 261 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M V V L S V P A E V T V I L L D I E G T T T P I A F V K D I L F P Y I E E N V K E Y L Q T H W E E E E C Q Q D V S L L R K Q A E E D A H L D G A V P I P A A S G N G V D D L Q Q M I Q A V V D N V C W Q M S L D R K T T A L K Q L Q G H M W R A A F T A G R M K A E F F A D V V P A V R K W R E A G M K V Y I Y S S G S V E A Q K L L F G H S T E G D I L E L V D G H F D T K I G H K V E S E S Y R K I A D S I G C S T N N I L F L T D V T R E A S A A E E A D V H V A V V V R P G N A G L T D D E K T Y Y S L I T S F S E L Y L P S S T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
Alternative names
MASA, MSTP145, ENOPH1, Enolase-phosphatase E1, MASA homolog
Recommended products
Recombinant Human MASA protein is a Human Full Length protein, in the 1 to 261 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9UHY7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M V V L S V P A E V T V I L L D I E G T T T P I A F V K D I L F P Y I E E N V K E Y L Q T H W E E E E C Q Q D V S L L R K Q A E E D A H L D G A V P I P A A S G N G V D D L Q Q M I Q A V V D N V C W Q M S L D R K T T A L K Q L Q G H M W R A A F T A G R M K A E F F A D V V P A V R K W R E A G M K V Y I Y S S G S V E A Q K L L F G H S T E G D I L E L V D G H F D T K I G H K V E S E S Y R K I A D S I G C S T N N I L F L T D V T R E A S A A E E A D V H V A V V V R P G N A G L T D D E K T Y Y S L I T S F S E L Y L P S S T
- Accession
- Q9UHY7
- Protein length
- Full Length
- Predicted molecular weight
- 31 kDa
- Amino acids
- 1 to 261
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab105128 is purified using conventional chromatography techniques.
General info
- Function
Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
- Sequence similarities
Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MASA protein also known simply as MASA presents a molecular mass of approximately 60 kDa. MASA is expressed predominantly in neuronal tissues with specific abundance in areas of the brain linked to cognitive functions. This protein resides within the cytoplasm and shows a strong presence in neurons suggesting its role in nervous system operations. The MASA protein has gathered significant interest in neuroscience for its contribution to cellular processes.
- Biological function summary
The MASA protein plays a critical role in cellular communication within neurons. It does not generally form part of a larger protein complex but interacts directly with other proteins to modulate synaptic transmission. Researchers have demonstrated that it influences the release of neurotransmitters impacting neuronal excitability and synaptic plasticity. This protein through its actions is vital for maintaining the functional network among neural cells.
- Pathways
MASA protein involves itself in signaling pathways related to synaptic function and plasticity. One of the key pathways includes the NMDA receptor pathway where MASA interacts with proteins like PSD-95. There is also significant interaction with proteins involved in the MAPK signaling pathway. The ability of MASA to influence these pathways points to its integral function in regulating synaptic strength and memory formation in the brain.
- Associated diseases and disorders
Researchers have found associations between MASA protein and certain neurodegenerative diseases. Alzheimer's disease presents strong connections with MASA as the protein appears to influence amyloid-beta processing. In addition the MASA protein has possible links to schizophrenia potentially connected through its interactions with disrupted-in-schizophrenia-1 (DISC1) proteins. These relationships highlight MASA as a potential target for therapeutic strategies aimed at treating neurological disorders.
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1 product image
SDS-PAGE - Recombinant Human MASA protein (ab105128)
15% SDS-PAGE showing ab105128 at approximately 31kDa (3μg).
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