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AB159246

Recombinant Human MASP1 protein (GST tag N-Terminus)

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Recombinant Human MASP1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 380 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

CRARF, CRARF1, PRSS5, MASP1, Mannan-binding lectin serine protease 1, Complement-activating component of Ra-reactive factor, Mannose-binding lectin-associated serine protease 1, Mannose-binding protein-associated serine protease, Ra-reactive factor serine protease p100, Serine protease 5, MASP-1, RaRF

1 Images
SDS-PAGE - Recombinant Human MASP1 protein (GST tag N-Terminus) (AB159246)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MASP1 protein (GST tag N-Terminus) (AB159246)

ab159246 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

P48740

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

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SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Primary Antibodies

AB276132

Anti-Angiotensinogen antibody [EPR24118-2]

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Immunocytochemistry/ Immunofluorescence - Anti-Angiotensinogen antibody [EPR24118-2] (AB276132)

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Primary Antibodies

AB255814

Anti-MASP1 antibody [EPR22680-36]

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Immunocytochemistry/ Immunofluorescence - Anti-MASP1 antibody [EPR22680-36] (AB255814)

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Proteins & Peptides

AB316432

Recombinant Human MASP2 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human MASP2 Protein Standard (His tag) (AB316432)

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Primary Antibodies

AB277520

Anti-MASP2 antibody [EPR23588-44]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASP2 antibody [EPR23588-44] (AB277520)

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  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MRWLLLYYALCFSLSKASAHTVELNNMFGQIQSPGYPDSYPSDSEVTWNITVPDGFRIKLYFMHFNLESSYLCEYDYVKVETEDQVLATFCGRETTDTEQTPGQEVVLSPGSFMSITFRSDFSNEERFTGFDAHYMAVDVDECKEREDEELSCDHYCHNYIGGYYCSCRFGYILHTDNRTCRVECSDNLFTQRTGVITSPDFPNPYPKSSECLYTIELEEGFMVNLQFEDIFDIEDHPEVPCPYDYIKIKVGPKVLGPFCGEKAPEPISTQSHSVLILFHSDNSGENRGWRLSYRAAGNECPELQPPVHGKIEPSQAKYFFKDQVLVSCDTGYKVLKDNVEMDTFQIECLKDGTWSNKIPTCKKNEIDLESELKSEQVTE","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":380,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P48740","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MASP1 protein also known as mannan-binding lectin serine protease 1 or MASP-1 is a serine protease with a mass of approximately 76 kDa. It is mainly expressed in the liver and is secreted into the blood. MASP1 is an important component of the lectin pathway of the complement system. This protein is an enzyme that circulates in the blood in an inactive form and becomes active when it binds to pathogen surfaces linked to mannose-binding lectins or ficolins.
Biological function summary

MASP1 plays a vital role in the innate immune system by activating the complement cascade. MASP1 works as part of a complex made up of pattern recognition molecules and other proteases. On binding to pathogens the complex undergoes conformational changes that activate MASP1. This activation leads to the cleavage of downstream substrates including MASP-2 and complement component C4 triggering a series of enzymatic reactions that enhance the pathogen clearance by opsonization and lysis.

Pathways

MASP1 is heavily involved in the lectin pathway one of the three activation pathways of the complement system. It shares functional ties with MASP-2 which continues the cascade after MASP1 activates the pathway. While the lectin pathway runs parallel to the classical pathway it is distinguished by the nature of its initiating molecules that recognize carbohydrate patterns on pathogen surfaces without needing antibodies. MASP1’s role is critical in facilitating the initial steps of this process which eventually leads to the activation of the terminal complement pathway components.

MASP1 shows connections to immune-related conditions such as systemic lupus erythematosus (SLE) and infectious diseases. Its dysregulation or deficiency can impact the complement system leading to immune complex diseases like SLE due to the failure in clearing immune complexes effectively. Furthermore MASP1’s association with MASP-2 also makes it relevant in studies focused on lectin pathway deficiencies that result in increased susceptibility to infections highlighting its significance in maintaining host immunity.
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Specifications

Form

Liquid

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General info

Function

Precursor of a serum protease that activates the complement pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.. Isoform MASP-1. Serine protease that activates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 10946292, PubMed : 11527969, PubMed : 12538697, PubMed : 22691502, PubMed : 22966085, PubMed : 9087411). The lectin complement system is activated following association of lectins, such as MBL2, FCN1, FCN2 or FCN3, to carbohydrates on the pathogen surface (PubMed : 22854970, PubMed : 22966085, PubMed : 23386610, PubMed : 9087411). Following autoproteolytic processing in response to lectin-binding to pathogen carbohydrates, catalyzes cleavage and activation of MASP2, the next component of the lectin complement pathway (PubMed : 22691502, PubMed : 22966085, PubMed : 23386610).. Isoform MASP-3. Serine protease that activates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 20038603, PubMed : 23792966, PubMed : 27535802). The alternative complement pathway acts as an amplification loop that enhances other complement pathways (PubMed : 27535802). Isoform MASP-3 specifically catalyzes cleavage and activation of factor D (CFD), the protease that initiates the alternative complement pathway (PubMed : 20038603, PubMed : 27535802).

Sequence similarities

Belongs to the peptidase S1 family.

Post-translational modifications

Isoform MASP-1. Autoproteolytic processing of the proenzyme produces the active enzyme composed on the heavy and the light chain held together by a disulfide bond.. Isoform MASP-3. In contrast to isoform MASP-1, not activated through autoproteolytic processing.

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Product protocols

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Target data

Precursor of a serum protease that activates the complement pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.. Isoform MASP-1. Serine protease that activates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 10946292, PubMed : 11527969, PubMed : 12538697, PubMed : 22691502, PubMed : 22966085, PubMed : 9087411). The lectin complement system is activated following association of lectins, such as MBL2, FCN1, FCN2 or FCN3, to carbohydrates on the pathogen surface (PubMed : 22854970, PubMed : 22966085, PubMed : 23386610, PubMed : 9087411). Following autoproteolytic processing in response to lectin-binding to pathogen carbohydrates, catalyzes cleavage and activation of MASP2, the next component of the lectin complement pathway (PubMed : 22691502, PubMed : 22966085, PubMed : 23386610).. Isoform MASP-3. Serine protease that activates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 20038603, PubMed : 23792966, PubMed : 27535802). The alternative complement pathway acts as an amplification loop that enhances other complement pathways (PubMed : 27535802). Isoform MASP-3 specifically catalyzes cleavage and activation of factor D (CFD), the protease that initiates the alternative complement pathway (PubMed : 20038603, PubMed : 27535802).
See full target information MASP1
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