Recombinant Human MASPIN protein (His tag) is a Human Full Length protein, in the 1 to 375 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M D A L Q L A N S A F A V D L F K Q L C E K E P L G N V L F S P I C L S T S L S L A Q V G A K G D T A N E I G Q V L H F E N V K D V P F G F Q T V T S D V N K L S S F Y S L K L I K R L Y V D K S L N L S T E F I S S T K R P Y A K E L E T V D F K D K L E E T K G Q I N N S I K D L T D G H F E N I L A D N S V N D Q T K I L V V N A A Y F V G K W M K K F S E S E T K E C P F R V N K T D T K P V Q M M N M E A T F C M G N I D S I N C K I I E L P F Q N K H L S M F I L L P K D V E D E S T G L E K I E K Q L N S E S L S Q W T N P S T M A N A K V K L S I P K F K V E K M I D P K A C L E N L G L K H I F S E D T S D F S G M S E T K G V A L S N V I H K V C L E I T E D G G D S I E V P G A R I L Q H K D E L N A D H P F I Y I I R H N K T R N I I F F G K F C S P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Alternative names
PI5, SERPINB5, Serpin B5, Maspin, Peptidase inhibitor 5, PI-5
Recommended products
Recombinant Human MASPIN protein (His tag) is a Human Full Length protein, in the 1 to 375 aa range, expressed in Escherichia coli, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >98% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P36952-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
- Concentration
- Storage buffer
pH: 8
Preservative: 0.8% Proclin 300
Constituents: 79.82% Trehalose, 14.02% Sodium chloride, 3.86% Tris, 0.46% EDTA, 0.24% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.16% Sodium-N-Lauroylsarcosinate
Sequence info
Amino acid sequence
- M D A L Q L A N S A F A V D L F K Q L C E K E P L G N V L F S P I C L S T S L S L A Q V G A K G D T A N E I G Q V L H F E N V K D V P F G F Q T V T S D V N K L S S F Y S L K L I K R L Y V D K S L N L S T E F I S S T K R P Y A K E L E T V D F K D K L E E T K G Q I N N S I K D L T D G H F E N I L A D N S V N D Q T K I L V V N A A Y F V G K W M K K F S E S E T K E C P F R V N K T D T K P V Q M M N M E A T F C M G N I D S I N C K I I E L P F Q N K H L S M F I L L P K D V E D E S T G L E K I E K Q L N S E S L S Q W T N P S T M A N A K V K L S I P K F K V E K M I D P K A C L E N L G L K H I F S E D T S D F S G M S E T K G V A L S N V I H K V C L E I T E D G G D S I E V P G A R I L Q H K D E L N A D H P F I Y I I R H N K T R N I I F F G K F C S P
- Accession
- P36952
- Protein length
- Full Length
- Predicted molecular weight
- 45.8 kDa
- Amino acids
- 1 to 375
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
- Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MASPIN also known as SERPINB5 is a non-inhibitory serine protease inhibitor with a mass of approximately 42 kDa. It functions mechanically to regulate cell adhesion migration apoptosis and angiogenesis. MASPIN localizes mainly in the cytoplasm and nucleus and expresses predominantly in epithelial cells including those in the mammary gland prostate and keratinocytes in the skin. It acts as a tumor suppressor and researchers often use immunohistochemistry (IHC) to study its expression patterns in various tissues.
- Biological function summary
MASPIN influences apoptosis and cell motility playing a critical role in tumor suppression. It does not form part of a complex but interacts directly with extracellular matrix components. MASPIN can inhibit angiogenesis by reducing the proliferation and migration of endothelial cells. Scientists have noted its role in maintaining normal cell architecture and inhibiting tumor cell invasion.
- Pathways
Researchers place MASPIN in important signaling networks impacting cancer progression and metastasis. It participates in the PI3K/AKT pathway thereby influencing cell growth and survival. MASPIN also intersects with TGF-β signaling and related proteins like SMAD which mediate growth arrest and apoptosis. By altering these pathways MASPIN helps modulate responses to cellular stress and inhibit lethal growth in cancer cells.
- Associated diseases and disorders
MASPIN often associates with breast and prostate cancers. Its expression inversely correlates with tumor progression suggesting a protective role. MASPIN's interaction with proteins like p53 highlights its contribution to tumor suppression. Abnormal MASPIN presence links to aggressive disease phenotypes making it a potential biomarker for prognostic evaluation in oncology.
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SDS-PAGE - Recombinant Human MASPIN protein (His tag) (ab275794)
SDS-PAGE analysis of ab275794.
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