Recombinant Human Mast Cell Tryptase protein (denatured) is a Human Full Length protein, in the 31 to 275 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M I V G G Q E A P R S K W P W Q V S L R V H G P Y W M H F C G G S L I H P Q W V L T A A H C V G P D V K D L A A L R V Q L R E Q H L Y Y Q D Q L L P V S R I I V H P Q F Y T A Q I G A D I A L L E L E E P V N V S S H V H T V T L P P A S E T F P P G M P C W V T G W G D V D N D E R L P P P F P L K Q V K V P I M E N H I C D A K Y H L G A Y T G D D V R I V R D D M L C A G N T R R D S C Q G D S G G P L V C K V N G T W L Q A G V V S W G E G C A Q P N R P G I Y T R V T Y Y L D W I H H Y V P K K P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315).
Alternative names
Tryptase alpha/beta-1, Tryptase-1, Tryptase I, Tryptase alpha-1, TPSB1, TPS1, TPS2, TPSAB1
Recommended products
Recombinant Human Mast Cell Tryptase protein (denatured) is a Human Full Length protein, in the 31 to 275 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q15661-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M I V G G Q E A P R S K W P W Q V S L R V H G P Y W M H F C G G S L I H P Q W V L T A A H C V G P D V K D L A A L R V Q L R E Q H L Y Y Q D Q L L P V S R I I V H P Q F Y T A Q I G A D I A L L E L E E P V N V S S H V H T V T L P P A S E T F P P G M P C W V T G W G D V D N D E R L P P P F P L K Q V K V P I M E N H I C D A K Y H L G A Y T G D D V R I V R D D M L C A G N T R R D S C Q G D S G G P L V C K V N G T W L Q A G V V S W G E G C A Q P N R P G I Y T R V T Y Y L D W I H H Y V P K K P
- Accession
- Protein length
Full Length
- Predicted molecular weight
30.1 kDa
- Amino acids
31 to 275
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates (PubMed:18854315).
- Sequence similarities
Belongs to the peptidase S1 family. Tryptase subfamily.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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2 product images
SDS-PAGE - Recombinant Human Mast Cell Tryptase protein (denatured) (ab134529)
15% SDS-PAGE analysis of 3 μg ab134529.
Western blot - Recombinant Human Mast Cell Tryptase protein (denatured) (ab134529)
Anti-Mast Cell Chymase antibody [CC1] ab2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.
All lanes: Western blot - Anti-Mast Cell Chymase antibody [CC1] (Anti-Mast Cell Chymase antibody [CC1] ab2377) at 1 µg/mL
Lane 1: Recombinant Human Chymase Protein at 0.1 µg
Lane 2: Western blot - Recombinant Human Mast Cell Tryptase protein (denatured) (ab134529) at 0.1 µg
SecondaryAll lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 35 kDa
Exposure time: 30s
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