Recombinant Human MAT2B/TGR protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine (PubMed:10644686, PubMed:23189196, PubMed:25075345). Can bind NADP (in vitro) (PubMed:23189196, PubMed:23425511).
Alternative names
TGR, MSTP045, Nbla02999, UNQ2435/PRO4995, MAT2B, Methionine adenosyltransferase 2 subunit beta, Methionine adenosyltransferase II beta, Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase, MAT II beta
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Recombinant Human MAT2B/TGR protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9NZL9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Specifications
- Form
- Liquid
- Additional notes
ab89357 is purified using conventional chromatography techniques.
General info
- Function
Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine (PubMed:10644686, PubMed:23189196, PubMed:25075345). Can bind NADP (in vitro) (PubMed:23189196, PubMed:23425511).
- Sequence similarities
Belongs to the dTDP-4-dehydrorhamnose reductase family. MAT2B subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MAT2B also known as TGR is a protein that functions as a regulatory subunit of the enzyme methionine adenosyltransferase II. This enzyme synthesizes S-adenosylmethionine (SAM) essential for methylation reactions in varied cellular processes. MAT2B has a molecular mass of approximately 35 kDa and can modulate the activity of the catalytic subunit of the methionine adenosyltransferase complex. The protein is expressed in multiple tissues with abundant levels in liver and fetal tissues. It is important for regulating the levels of SAM linking dietary methionine to cellular methylation capacity.
- Biological function summary
The MAT2B protein helps maintain cellular methylation status by adjusting SAM synthesis. It is a part of the methionine adenosyltransferase II complex which consists of different subunits contributing to its enzymatic activity. The regulatory role of MAT2B ensures precise control over the methionine cycle impacting DNA methylation patterns and gene regulation. By modulating SAM production MAT2B influences cellular growth differentiation and apoptosis through its interactions with other methylation-related proteins.
- Pathways
The MAT2B protein plays a significant role in methionine metabolism and the transmethylation pathway. These pathways are central to transferring methyl groups from SAM to a range of substrates including DNA proteins and lipids. MAT2B is related to MAT2A the catalytic subunit that generates SAM. Together they coordinate to balance cellular metabolic needs with the synthesis and utilization of methyl groups securing the methylation-dependent regulation of diverse cellular processes.
- Associated diseases and disorders
MAT2B associates with liver diseases and cancer. Its regulatory function in SAM synthesis ties it to disruptions in methylation capacity observed in these conditions. Reduced MAT2B activity can lead to imbalanced SAM levels affecting the methylation landscape and contributing to altered gene expression involved in tumorigenesis. In liver diseases MAT2B interacts with MAT1A a protein facilitating methylation balance in mature liver tissue. This interaction highlights MAT2B's significant impact on maintaining liver health and its potential role in pathogenesis when dysregulated.
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1 product image
SDS-PAGE - Recombinant Human MAT2B/TGR protein (ab89357)
15% SDS-PAGE analysis of 3μg ab89357.
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Product protocols
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