Recombinant human MATK protein is a Human Full Length protein, in the 1 to 507 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A G R G S L V S W R A F H G C D S A E E L P R V S P R F L R A W H P P P V S A R M P T R R W A P G T Q C I T K C E H T R P K P G E L A F R K G D V V T I L E A C E N K S W Y R V K H H T S G Q E G L L A A G A L R E R E A L S A D P K L S L M P W F H G K I S G Q E A V Q Q L Q P P E D G L F L V R E S A R H P G D Y V L C V S F G R D V I H Y R V L H R D G H L T I D E A V F F C N L M D M V E H Y S K D K G A I C T K L V R P K R K H G T K S A E E E L A R A G W L L N L Q H L T L G A Q I G E G E F G A V L Q G E Y L G Q K V A V K N I K C D V T A Q A F L D E T A V M T K M Q H E N L V R L L G V I L H Q G L Y I V M E H V S K G N L V N F L R T R G R A L V N T A Q L L Q F S L H V A E G M E Y L E S K K L V H R D L A A R N I L V S E D L V A K V S D F G L A K A E R K G L D S S R L P V K W T A P E A L K H G K F T S K S D V W S F G V L L W E V F S Y G R A P Y P K M S L K E V S E A V E K G Y R M E P P E G C P G P V H V L M S S C W E A E P A R R P P F R K L A E K L A R E L R S A G A P A S V S G Q D A D G S T S P R S Q E P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.
Alternative names
CTK, HYL, MATK, Megakaryocyte-associated tyrosine-protein kinase, CSK homologous kinase, Hematopoietic consensus tyrosine-lacking kinase, Protein kinase HYL, Tyrosine-protein kinase CTK, CHK
Recommended products
Recombinant human MATK protein is a Human Full Length protein, in the 1 to 507 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of MATK was determined to be 11 nmol /min/mg as per activity assay protocol.
- Accession
- P42679-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M A G R G S L V S W R A F H G C D S A E E L P R V S P R F L R A W H P P P V S A R M P T R R W A P G T Q C I T K C E H T R P K P G E L A F R K G D V V T I L E A C E N K S W Y R V K H H T S G Q E G L L A A G A L R E R E A L S A D P K L S L M P W F H G K I S G Q E A V Q Q L Q P P E D G L F L V R E S A R H P G D Y V L C V S F G R D V I H Y R V L H R D G H L T I D E A V F F C N L M D M V E H Y S K D K G A I C T K L V R P K R K H G T K S A E E E L A R A G W L L N L Q H L T L G A Q I G E G E F G A V L Q G E Y L G Q K V A V K N I K C D V T A Q A F L D E T A V M T K M Q H E N L V R L L G V I L H Q G L Y I V M E H V S K G N L V N F L R T R G R A L V N T A Q L L Q F S L H V A E G M E Y L E S K K L V H R D L A A R N I L V S E D L V A K V S D F G L A K A E R K G L D S S R L P V K W T A P E A L K H G K F T S K S D V W S F G V L L W E V F S Y G R A P Y P K M S L K E V S E A V E K G Y R M E P P E G C P G P V H V L M S S C W E A E P A R R P P F R K L A E K L A R E L R S A G A P A S V S G Q D A D G S T S P R S Q E P
- Accession
- P42679
- Protein length
- Full Length
- Predicted molecular weight
- 83 kDa
- Amino acids
- 1 to 507
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Active Poly (Glu, Tyr) peptide ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MATK also known as Megakaryocyte-associated Tyrosine Kinase is a non-receptor tyrosine kinase with an approximate mass of 72 kDa. This protein is mainly expressed in hematopoietic cells especially in megakaryocytes where it plays a significant role in cellular signaling. MATK shares structural similarity with the Csk (C-terminal Src kinase) family suggesting its involvement in regulatory processes within these cells. Its expression is not limited to hematopoietic tissues as it is also found in various other cell types indicating its wider cellular function.
- Biological function summary
MATK participates in the negative regulation of Src family kinases (SFKs) by phosphorylating their inhibitory tyrosine residues. This action modulates the activity of SFKs and influences processes such as cell proliferation differentiation and migration. MATK is often involved in complexes with other signaling molecules playing a regulatory function in cellular events. Its exact role can vary depending on specific cellular contexts and signals.
- Pathways
More than four words make up the signaling landscape that MATK influences. It is an important player in both the hematopoietic and immune cell pathways. In the hematopoietic pathway MATK regulates SFKs ensuring controlled cell division and differentiation. In the immune response its regulation of kinases affects how immune cells react to stimuli. Proteins such as LCK and Fyn members of the SFK family are directly impacted by MATK’s activity within these pathways highlighting its importance in cellular function.
- Associated diseases and disorders
Alterations in more than four words in MATK expression or function have been linked to hematological malignancies and immune system disorders. Dysregulation of MATK is associated with certain leukemias where its impaired function leads to unchecked cell proliferation. Similarly improper regulation in the immune context can contribute to autoimmune diseases. Its relationship with Src family kinases particularly LCK and Fyn cements MATK's role in oncogenic processes and autoimmune conditions providing potential targets for therapeutic intervention.
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4 product images
Functional Studies - Recombinant human MATK protein (ab204136)
The specific activity of MATK (ab204136) was determined to be 3.8 nmol/min/mg as per activity assay protocol and was equivalent to 12 nmol/min/mg as per radiometric assay
SDS-PAGE - Recombinant human MATK protein (ab204136)
SDS PAGE analysis of ab204136
Functional Studies - Recombinant human MATK protein (ab204136)
The specific activity of ab204136 was determined to be 11 nmol /min/mg.
SDS-PAGE - Recombinant human MATK protein (ab204136)
SDS-PAGE showing ab204136.
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