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AB204136

Recombinant human MATK protein

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Recombinant human MATK protein is a Human Full Length protein, in the 1 to 507 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.

View Alternative Names

CTK, HYL, MATK, Megakaryocyte-associated tyrosine-protein kinase, CSK homologous kinase, Hematopoietic consensus tyrosine-lacking kinase, Protein kinase HYL, Tyrosine-protein kinase CTK, CHK

4 Images
Functional Studies - Recombinant human MATK protein (AB204136)
  • FuncS

Supplier Data

Functional Studies - Recombinant human MATK protein (AB204136)

The specific activity of ab204136 was determined to be 11 nmol /min/mg.

Functional Studies - Recombinant human MATK protein (AB204136)
  • FuncS

Unknown

Functional Studies - Recombinant human MATK protein (AB204136)

The specific activity of MATK (ab204136) was determined to be 3.8 nmol/min/mg as per activity assay protocol and was equivalent to 12 nmol/min/mg as per radiometric assay

SDS-PAGE - Recombinant human MATK protein (AB204136)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MATK protein (AB204136)

SDS PAGE analysis of ab204136

SDS-PAGE - Recombinant human MATK protein (AB204136)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human MATK protein (AB204136)

SDS-PAGE showing ab204136.

Key facts

Purity

>85% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of MATK was determined to be 11 nmol /min/mg as per activity assay protocol.

Accession

P42679

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"MAGRGSLVSWRAFHGCDSAEELPRVSPRFLRAWHPPPVSARMPTRRWAPGTQCITKCEHTRPKPGELAFRKGDVVTILEACENKSWYRVKHHTSGQEGLLAAGALREREALSADPKLSLMPWFHGKISGQEAVQQLQPPEDGLFLVRESARHPGDYVLCVSFGRDVIHYRVLHRDGHLTIDEAVFFCNLMDMVEHYSKDKGAICTKLVRPKRKHGTKSAEEELARAGWLLNLQHLTLGAQIGEGEFGAVLQGEYLGQKVAVKNIKCDVTAQAFLDETAVMTKMQHENLVRLLGVILHQGLYIVMEHVSKGNLVNFLRTRGRALVNTAQLLQFSLHVAEGMEYLESKKLVHRDLAARNILVSEDLVAKVSDFGLAKAERKGLDSSRLPVKWTAPEALKHGKFTSKSDVWSFGVLLWEVFSYGRAPYPKMSLKEVSEAVEKGYRMEPPEGCPGPVHVLMSSCWEAEPARRPPFRKLAEKLARELRSAGAPASVSGQDADGSTSPRSQEP","proteinLength":"Full Length","predictedMolecularWeight":"83 kDa","actualMolecularWeight":null,"aminoAcidEnd":507,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P42679","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MATK also known as Megakaryocyte-associated Tyrosine Kinase is a non-receptor tyrosine kinase with an approximate mass of 72 kDa. This protein is mainly expressed in hematopoietic cells especially in megakaryocytes where it plays a significant role in cellular signaling. MATK shares structural similarity with the Csk (C-terminal Src kinase) family suggesting its involvement in regulatory processes within these cells. Its expression is not limited to hematopoietic tissues as it is also found in various other cell types indicating its wider cellular function.
Biological function summary

MATK participates in the negative regulation of Src family kinases (SFKs) by phosphorylating their inhibitory tyrosine residues. This action modulates the activity of SFKs and influences processes such as cell proliferation differentiation and migration. MATK is often involved in complexes with other signaling molecules playing a regulatory function in cellular events. Its exact role can vary depending on specific cellular contexts and signals.

Pathways

It is an important player in both the hematopoietic and immune cell pathways. In the hematopoietic pathway MATK regulates SFKs ensuring controlled cell division and differentiation. In the immune response its regulation of kinases affects how immune cells react to stimuli. Proteins such as LCK and Fyn members of the SFK family are directly impacted by MATK’s activity within these pathways highlighting its importance in cellular function.

Dysregulation of MATK is associated with certain leukemias where its impaired function leads to unchecked cell proliferation. Similarly improper regulation in the immune context can contribute to autoimmune diseases. Its relationship with Src family kinases particularly LCK and Fyn cements MATK's role in oncogenic processes and autoimmune conditions providing potential targets for therapeutic intervention.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.

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Product protocols

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Target data

Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.
See full target information MATK
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