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AB95309

Recombinant Human MAX protein

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(1 Publication)

Recombinant Human MAX protein is a Human Full Length protein, in the 1 to 160 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec, WB.

View Alternative Names

BHLHD4, MAX, Protein max, Class D basic helix-loop-helix protein 4, Myc-associated factor X, bHLHd4

3 Images
SDS-PAGE - Recombinant Human MAX protein (AB95309)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MAX protein (AB95309)

SDS-PAGE analysis of ab95309 (3µg).

Western blot - Recombinant Human MAX protein (AB95309)
  • WB

Unknown

Western blot - Recombinant Human MAX protein (AB95309)

ab68578 recognizes the tagged recombinant MAX protein (ab95309) which has an expected molecular weight of 24 kDa.

All lanes:

Anti-MAX antibody (<a href='/en-us/products/unavailable/max-antibody-ab68578'>ab68578</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human MAX protein (ab95309) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 8min

SDS-PAGE - Recombinant Human MAX protein (AB95309)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MAX protein (AB95309)

ab95309 at 3 μg on an SDS-PAGE gel (15%).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

WB, SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P61244

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab95309 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/max-antibody-ab68578'>ab68578</a>.</p>" } } }
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Sequence info

[{"sequence":"MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEASLEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"19.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":160,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P61244","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MAX protein also known as Myc-associated factor X functions mechanically as a transcription factor with a molecular weight of approximately 20 kDa. MAX is expressed in a wide range of tissues throughout the body. The protein forms homodimers or heterodimers with other members of the basic-helix-loop-helix-zipper (bHLHZ) family such as MYC MAD and MNT. The dimerization with different partners determines its role as an activator or repressor of transcription affecting the regulation of various genes.
Biological function summary

The MAX protein plays a significant role in controlling cell growth differentiation and apoptosis. The protein often forms part of complex regulatory networks within the cell. By interacting with different partner proteins MAX modulates transcriptional activity affecting cellular outcomes. Importantly it can switch between activating or repressing target genes depending on the dimer partner influencing processes such as proliferation and differentiation.

Pathways

MAX is deeply integrated into the MYC-MAX-MAD pathway an important pathway for regulating gene expression linked to cell cycle and proliferative processes. Within this pathway MAX pairs with either MYC or MAD depending on the cellular context to influence transcription. When paired with MYC it promotes transcription while pairing with MAD results in transcriptional repression. Additionally in the growth signaling pathway the MAX protein’s regulation of target genes is pivotal for ensuring proper cellular responses to growth signals.

The malfunction of the MAX protein has a strong association with cancer and neurodegenerative diseases. Alterations in MAX function can lead to uncontrolled cell growth contributing to oncogenesis particularly in relation to MYC which is a known oncogene. Furthermore dysregulation of the MAX protein is linked to neurodegenerative disorders where abnormal MAX activity may disrupt normal neural gene expression enhancing disease progression.
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Specifications

Form

Liquid

Additional notes

ab95309 is purified using conventional chromatography techniques.

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General info

Function

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC : MAX complex is a transcriptional activator, whereas the MAD : MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.

Sequence similarities

Belongs to the MAX family.

Post-translational modifications

Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC : MAX complex is a transcriptional activator, whereas the MAD : MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.
See full target information MAX
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell chemical biology 26:711-723.e14 PubMed30880155

2019

Stabilization of the Max Homodimer with a Small Molecule Attenuates Myc-Driven Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas B Struntz,Andrew Chen,Anja Deutzmann,Robert M Wilson,Eric Stefan,Helen L Evans,Maricela A Ramirez,Tong Liang,Francisco Caballero,Mattheus H E Wildschut,Dylan V Neel,David B Freeman,Marius S Pop,Marie McConkey,Sandrine Muller,Brice H Curtin,Hanna Tseng,Kristen R Frombach,Vincent L Butty,Stuart S Levine,Clementine Feau,Sarah Elmiligy,Jiyoung A Hong,Timothy A Lewis,Amedeo Vetere,Paul A Clemons,Scott E Malstrom,Benjamin L Ebert,Charles Y Lin,Dean W Felsher,Angela N Koehler
View all publications
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