Recombinant Human MAX protein is a Human Full Length protein, in the 1 to 160 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS, WB.
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Publications
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- Cell chemical biology 26:711-723.e142019Stabilization of the Max Homodimer with a Small Molecule Attenuates Myc-Driven Transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicholas B Struntz et. al.PubMed 30880155
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, MS, WB
- Biologically active
No
Amino acid sequence
M S D N D D I E V E S D E E Q P R F Q S A A D K R A H H N A L E R K R R D H I K D S F H S L R D S V P S L Q G E K A S R A Q I L D K A T E Y I Q Y M R R K N H T H Q Q D I D D L K R Q N A L L E Q Q V R A L E K A R S S A Q L Q T N Y P S S D N S L Y T N A K G S T I S A F D G G S D S S S E S E P E E P Q S R K K L R M E A S L E H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab95309 can be used as a WB positive control in conjunction with ab68578. |
Target data
Function
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.
Alternative names
BHLHD4, BHLHD4, MAX, Protein max, Class D basic helix-loop-helix protein 4, Myc-associated factor X, bHLHd4
Recommended products
Recombinant Human MAX protein is a Human Full Length protein, in the 1 to 160 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS, WB.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, MS, WB
- Biologically active
No
- Accession
- P61244-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M S D N D D I E V E S D E E Q P R F Q S A A D K R A H H N A L E R K R R D H I K D S F H S L R D S V P S L Q G E K A S R A Q I L D K A T E Y I Q Y M R R K N H T H Q Q D I D D L K R Q N A L L E Q Q V R A L E K A R S S A Q L Q T N Y P S S D N S L Y T N A K G S T I S A F D G G S D S S S E S E P E E P Q S R K K L R M E A S L E H H H H H H
- Accession
- Protein length
Full Length
- Predicted molecular weight
19.3 kDa
- Amino acids
1 to 160
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Liquid
- Additional notes
ab95309 is purified using conventional chromatography techniques.
General info
- Function
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.
- Sequence similarities
Belongs to the MAX family.
- Post-translational modifications
Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MAX protein also known as Myc-associated factor X functions mechanically as a transcription factor with a molecular weight of approximately 20 kDa. MAX is expressed in a wide range of tissues throughout the body. The protein forms homodimers or heterodimers with other members of the basic-helix-loop-helix-zipper (bHLHZ) family such as MYC MAD and MNT. The dimerization with different partners determines its role as an activator or repressor of transcription affecting the regulation of various genes.
- Biological function summary
The MAX protein plays a significant role in controlling cell growth differentiation and apoptosis. The protein often forms part of complex regulatory networks within the cell. By interacting with different partner proteins MAX modulates transcriptional activity affecting cellular outcomes. Importantly it can switch between activating or repressing target genes depending on the dimer partner influencing processes such as proliferation and differentiation.
- Pathways
MAX is deeply integrated into the MYC-MAX-MAD pathway an important pathway for regulating gene expression linked to cell cycle and proliferative processes. Within this pathway MAX pairs with either MYC or MAD depending on the cellular context to influence transcription. When paired with MYC it promotes transcription while pairing with MAD results in transcriptional repression. Additionally in the growth signaling pathway the MAX protein’s regulation of target genes is pivotal for ensuring proper cellular responses to growth signals.
- Associated diseases and disorders
The malfunction of the MAX protein has a strong association with cancer and neurodegenerative diseases. Alterations in MAX function can lead to uncontrolled cell growth contributing to oncogenesis particularly in relation to MYC which is a known oncogene. Furthermore dysregulation of the MAX protein is linked to neurodegenerative disorders where abnormal MAX activity may disrupt normal neural gene expression enhancing disease progression.
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3 product images
Western blot - Recombinant Human MAX protein (ab95309)
ab68578 recognizes the tagged recombinant MAX protein (ab95309) which has an expected molecular weight of 24 kDa.
All lanes: Anti-MAX antibody (ab68578) at 1 µg/mL
All lanes: Western blot - Recombinant Human MAX protein (ab95309) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 8min
SDS-PAGE - Recombinant Human MAX protein (ab95309)
ab95309 at 3 μg on an SDS-PAGE gel (15%).
SDS-PAGE - Recombinant Human MAX protein (ab95309)
SDS-PAGE analysis of ab95309 (3µg).
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