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AB134609

Recombinant Human MBD3 protein (His tag N-Terminus)

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Recombinant Human MBD3 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 291 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, MBD3

1 Images
SDS-PAGE - Recombinant Human MBD3 protein (His tag N-Terminus) (AB134609)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MBD3 protein (His tag N-Terminus) (AB134609)

15% SDS-PAGE analysis of ab134609 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O95983

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMERKRWECPALPQGWEREEVPRRSGLSAGHRDVFYYSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKMLMSKMNKSRQRVRYDSSNQVKGKPDLNTALPVRQTASIFKQPVTKITNHPSNKVKSDPQKAVDQPRQLFWEKKLSGLNAFDIAEELVKTMDLPKGLQGVGPGCTDETLLSAIASALHTSTMPITGQLSAAVEKNPGVWLNTTQPLCKAFMVTDEDIRKQEELVQQVRKRLEEALMADMLAHVEELARDGEAPLDKACAEDDDEEDEEEEEEEPDPDPEMEHV","proteinLength":"Full Length","predictedMolecularWeight":"35.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":291,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95983","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MBD3 protein also known as Methyl-CpG-binding domain protein 3 functions mechanically by binding to methylated DNA which is an essential step in regulating gene expression. It has a molecular mass of approximately 32 kDa. MBD3 is ubiquitously expressed in various tissues demonstrating its role in fundamental cellular processes. The protein does not function alone and often interacts with other proteins as part of gene repression mechanisms.
Biological function summary

The activity of MBD3 impacts chromatin remodeling and gene expression regulation. MBD3 operates as a core component of the nucleosome remodeling deacetylase (NuRD) complex a multi-protein assembly important for transcriptional repression and chromatin structure modification. Its interaction within this complex helps modulate gene expression by altering the accessibility of chromatin and recruiting other modifiers for further regulation.

Pathways

MBD3 plays a significant role in key signaling and epigenetic pathways. It engages in pathways related to chromatin modification and transcription regulation. Its role in the NuRD complex underlines its participation in transcriptional regulation pathways impacting proteins such as HDAC1 and GATA2 which contribute to modifying histone acetylation and influencing gene transcription processes.

Changes in MBD3 expression or function have links to cancer and neurodevelopmental disorders. MBD3 dysregulation associates with tumorigenesis due to its role in maintaining proper gene silencing and expression. Additionally impairment in MBD3 function can relate to intellectual disabilities and developmental delays potentially due to its interaction with epigenetic regulators like MeCP2.
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Specifications

Form

Liquid

Additional notes

ab134609 was purified using conventional chromatography techniques.

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General info

Function

Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 12124384, PubMed : 16428440, PubMed : 28977666). Acts as transcriptional repressor and plays a role in gene silencing (PubMed : 10947852, PubMed : 18644863). Does not bind to methylated DNA by itself (PubMed : 12124384, PubMed : 16428440). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed : 24307175). Recruits histone deacetylases and DNA methyltransferases.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 12124384, PubMed : 16428440, PubMed : 28977666). Acts as transcriptional repressor and plays a role in gene silencing (PubMed : 10947852, PubMed : 18644863). Does not bind to methylated DNA by itself (PubMed : 12124384, PubMed : 16428440). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed : 24307175). Recruits histone deacetylases and DNA methyltransferases.
See full target information MBD3
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