Recombinant Human MCAK protein is a Human Full Length protein, in the 1 to 725 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A M D S S L Q A R L F P G L A I K I Q R S N G L I H S A N V R T V N L E K S C V S V E W A E G G A T K G K E I D F D D V A A I N P E L L Q L L P L H P K D N L P L Q E N V T I Q K Q K R R S V N S K I P A P K E S L R S R S T R M S T V S E L R I T A Q E N D M E V E L P A A A N S R K Q F S V P P A P T R P S C P A V A E I P L R M V S E E M E E Q V H S I R G S S S A N P V N S V R R K S C L V K E V E K M K N K R E E K K A Q N S E M R M K R A Q E Y D S S F P N W E F A R M I K E F R A T L E C H P L T M T D P I E E H R I C V C V R K R P L N K Q E L A K K E I D V I S I P S K C L L L V H E P K L K V D L T K Y L E N Q A F C F D F A F D E T A S N E V V Y R F T A R P L V Q T I F E G G K A T C F A Y G Q T G S G K T H T M G G D L S G K A Q N A S K G I Y A M A S R D V F L L K N Q P C Y R K L G L E V Y V T F F E I Y N G K L F D L L N K K A K L R V L E D G K Q Q V Q V V G L Q E H L V N S A D D V I K M I D M G S A C R T S G Q T F A N S N S S R S H A C F Q I I L R A K G R M H G K F S L V D L A G N E R G A D T S S A D R Q T R M E G A E I N K S L L A L K E C I R A L G Q N K A H T P F R E S K L T Q V L R D S F I G E N S R T C M I A T I S P G I S S C E Y T L N T L R Y A D R V K E L S P H S G P S G E Q L I Q M E T E E M E A C S N G A L I P G N L S K E E E E L S S Q M S S F N E A M T Q I R E L E E K A M E E L K E I I Q Q G P D W L E L S E M T E Q P D Y D L E T F V N K A E S A L A Q Q A K H F S A L R D V I K A L R L A M Q L E E Q A S R Q I S S K K R P Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:21820309). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed:19060894). Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (PubMed:23891108).
Alternative names
KNSL6, KIF2C, Kinesin-like protein KIF2C, Kinesin-like protein 6, Mitotic centromere-associated kinesin, MCAK
Recommended products
Recombinant Human MCAK protein is a Human Full Length protein, in the 1 to 725 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q99661-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A M D S S L Q A R L F P G L A I K I Q R S N G L I H S A N V R T V N L E K S C V S V E W A E G G A T K G K E I D F D D V A A I N P E L L Q L L P L H P K D N L P L Q E N V T I Q K Q K R R S V N S K I P A P K E S L R S R S T R M S T V S E L R I T A Q E N D M E V E L P A A A N S R K Q F S V P P A P T R P S C P A V A E I P L R M V S E E M E E Q V H S I R G S S S A N P V N S V R R K S C L V K E V E K M K N K R E E K K A Q N S E M R M K R A Q E Y D S S F P N W E F A R M I K E F R A T L E C H P L T M T D P I E E H R I C V C V R K R P L N K Q E L A K K E I D V I S I P S K C L L L V H E P K L K V D L T K Y L E N Q A F C F D F A F D E T A S N E V V Y R F T A R P L V Q T I F E G G K A T C F A Y G Q T G S G K T H T M G G D L S G K A Q N A S K G I Y A M A S R D V F L L K N Q P C Y R K L G L E V Y V T F F E I Y N G K L F D L L N K K A K L R V L E D G K Q Q V Q V V G L Q E H L V N S A D D V I K M I D M G S A C R T S G Q T F A N S N S S R S H A C F Q I I L R A K G R M H G K F S L V D L A G N E R G A D T S S A D R Q T R M E G A E I N K S L L A L K E C I R A L G Q N K A H T P F R E S K L T Q V L R D S F I G E N S R T C M I A T I S P G I S S C E Y T L N T L R Y A D R V K E L S P H S G P S G E Q L I Q M E T E E M E A C S N G A L I P G N L S K E E E E L S S Q M S S F N E A M T Q I R E L E E K A M E E L K E I I Q Q G P D W L E L S E M T E Q P D Y D L E T F V N K A E S A L A Q Q A K H F S A L R D V I K A L R L A M Q L E E Q A S R Q I S S K K R P Q
- Accession
- Q99661
- Protein length
- Full Length
- Predicted molecular weight
- 107.7 kDa
- Amino acids
- 1 to 725
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:21820309). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed:19060894). Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (PubMed:23891108).
- Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. MCAK/KIF2 subfamily.
- Post-translational modifications
Phosphorylation by AURKB, regulates association with centromeres and kinetochores and the microtubule depolymerization activity.
- Subcellular localisation
- Cytoskeleton, Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MCAK protein also known as KIF2C (kinesin family member 2C) is a motor protein that makes depolymerization of microtubules. It serves an important role in the regulation of microtubule dynamics during mitosis. MCAK has a molecular mass of approximately 81 kDa. Expression of MCAK is mostly found in actively dividing cells such as those in tissues with high turnover rates including the skin intestinal lining and specific tumors. This protein is critical for the proper formation and functioning of the mitotic spindle during cell division.
- Biological function summary
The MCAK protein contributes to accurate chromosome segregation. MCAK performs its functions by binding to microtubule ends and removing tubulin subunits thereby aiding in microtubule disassembly. It functions within the mitotic spindle apparatus and ensures that chromosomes align properly and evenly separate to opposite poles of the dividing cell. This activity is not part of a complex but acts independently to correct improper microtubule attachments.
- Pathways
MCAK plays a significant role in mitotic and cell cycle pathways. It coordinates with other proteins like Aurora B kinase which is involved in correcting improper kinetochore-microtubule attachments to maintain chromosome stability during mitosis. MCAK interacts with proteins controlling cell cycle checkpoints overlapping with pathways ensuring cells do not progress with errors in chromosome segregation.
- Associated diseases and disorders
Aberrant regulation of the MCAK protein can lead to chromosomal instability often observed in cancers such as colorectal and breast cancer. This instability can drive tumorigenesis where loss of proper chromosome segregation leads to aneuploidy and cancer progression. In these cancers MCAK may interact with other proteins such as Aurora kinases revealing potential therapeutic targets for intervention in malignancies with defective mitotic processes.
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1 product image
SDS-PAGE - Recombinant Human MCAK protein (ab132006)
12.5% SDS-PAGE using ab132006 stained with Coomassie Blue.
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