Recombinant Human MCJ protein is a Human Full Length protein, in the 1 to 150 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A A R G V I A P V G E S L R Y A E Y L Q P S A K R P D A D V D Q Q G L V R S L I A V G L G V A A L A F A G R Y A F R I W K P L E Q V I T E T A K K I S T P S F S S Y Y K G G F E Q K M S R R E A G L I L G V S P S A G K A K I R T A H R R V M I L N H P D K G G S P Y V A A K I N E A K D L L E T T T K H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP (By similarity). Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9.
Alternative names
DNAJD1, GIG22, HSD18, DNAJC15, DnaJ homolog subfamily C member 15, Cell growth-inhibiting gene 22 protein, Methylation-controlled J protein, MCJ
Recommended products
Recombinant Human MCJ protein is a Human Full Length protein, in the 1 to 150 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9Y5T4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A A R G V I A P V G E S L R Y A E Y L Q P S A K R P D A D V D Q Q G L V R S L I A V G L G V A A L A F A G R Y A F R I W K P L E Q V I T E T A K K I S T P S F S S Y Y K G G F E Q K M S R R E A G L I L G V S P S A G K A K I R T A H R R V M I L N H P D K G G S P Y V A A K I N E A K D L L E T T T K H
- Accession
- Q9Y5T4
- Protein length
- Full Length
- Amino acids
- 1 to 150
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP (By similarity). Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9.
- Subcellular localisation
- Mitochondrion inner membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as DNAJC15.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCJ also known as DNAJC15 is a mitochondrial protein with an approximate mass of 17 kDa. It serves various functions via its presence in different tissues including the liver and muscle. MCJ belongs to the J-protein family and it regulates protein folding and mitochondrial function. Structurally MCJ acts as a co-chaperone and influences mitochondrial respiration by inhibiting the mitochondrial respiratory chain complex I.
- Biological function summary
The role of MCJ extends beyond its mechanical actions. The protein participates in an intricate process that affects the cell's metabolism and energy production. MCJ often functions as part of a complex involving several mitochondrial proteins. It regulates mitochondrial energy production by inhibiting the activity of respiratory chain complexes leading to a direct impact on ATP synthesis and cellular metabolism.
- Pathways
MCJ influences mitochondrial bioenergetics and oxidative stress pathways. It is intertwined with the mitochondrial respiratory chain pathway where it modulates the activity of complex I thereby affecting energy production and cell survival. MCJ interacts with other proteins such as NDUFA9 a component of complex I and plays a role in maintaining mitochondrial efficiency and reducing oxidative damage under stress conditions.
- Associated diseases and disorders
MCJ is associated with cancer and metabolic syndrome. Decreased expression of MCJ has been linked with various cancers due to its impact on cell survival and proliferation. In metabolic disorders alterations in MCJ expression affect energy metabolism and insulin sensitivity. Related proteins like the anti-apoptotic Bcl-2 family can further connect MCJ to these conditions illustrating the protein’s influence on disease-related mitochondrial dysfunction.
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1 product image
SDS-PAGE - Recombinant Human MCJ protein (ab162220)
ab162220 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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