Recombinant Human MCL1 protein is a Human Fragment protein, in the 1 to 327 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Publications
Filter by
- Journal of medicinal chemistry 61:2962-29722018Bicyclic Helical Peptides as Dual Inhibitors Selective for Bcl2A1 and Mcl-1 Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAline D de Araujo et. al.PubMed 29584430
- Oncotarget 7:78958-789702016Mitotic arrest-induced phosphorylation of Mcl-1 revisited using two-dimensional gel electrophoresis and phosphoproteomics: nine phosphorylation sites identified.Applications:IP, WBReactive species:HumanRong Chu,Sarah E Alford,Katherine Hart,Anisha Kothari,Samuel G Mackintosh,Matthew R Kovak,Timothy C ChambersPubMed 27738316
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M F G L K R N A V I G L N L Y C G G A G L G A G S G G A T R P G G R L L A T E K E A S A R R E I G G G E A G A V I G G S A G A S P P S T L T P D S R R V A R P P P I G A E V P D V T A T P A R L L F F A P T R R A A P L E E M E A P A A D A I M S P E E E L D G Y E P E P L G K R P A V L P L L E L V G E S G N N T S T D G S L P S T P P P A E E E E D E L Y R Q S L E I I S R Y L R E Q A T G A K D T K P M G R S G A T S R K A L E T L R R V G D G V Q R N H E T A F Q G M L R K L D I K N E D D V K S L S R V M I H V F S D G V T N W G R I V T L I S F G A F V A K H L K T I N Q E S C I E P L A E S I T D V L V R T K R D W L V K Q R G W D G F V E F F H V E D L E G G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Alternative names
BCL2L3, MCL1, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, Bcl2-L-3
Recommended products
Recombinant Human MCL1 protein is a Human Fragment protein, in the 1 to 327 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q07820-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M F G L K R N A V I G L N L Y C G G A G L G A G S G G A T R P G G R L L A T E K E A S A R R E I G G G E A G A V I G G S A G A S P P S T L T P D S R R V A R P P P I G A E V P D V T A T P A R L L F F A P T R R A A P L E E M E A P A A D A I M S P E E E L D G Y E P E P L G K R P A V L P L L E L V G E S G N N T S T D G S L P S T P P P A E E E E D E L Y R Q S L E I I S R Y L R E Q A T G A K D T K P M G R S G A T S R K A L E T L R R V G D G V Q R N H E T A F Q G M L R K L D I K N E D D V K S L S R V M I H V F S D G V T N W G R I V T L I S F G A F V A K H L K T I N Q E S C I E P L A E S I T D V L V R T K R D W L V K Q R G W D G F V E F F H V E D L E G G
- Accession
- Q07820
- Protein length
- Fragment
- Predicted molecular weight
- 37.2 kDa
- Amino acids
- 1 to 327
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab131682 was purified using conventional chromatography techniques.
General info
- Function
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
- Sequence similarities
Belongs to the Bcl-2 family.
- Post-translational modifications
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
- Subcellular localisation
- Mitochondrion, Nucleus, Nucleoplasm
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCL1 also known as myeloid cell leukemia sequence 1 is an anti-apoptotic member of the BCL-2 family. This protein plays an important role in regulating cell survival by inhibiting apoptosis. MCL1 has a molecular weight of approximately 37 kDa. It is primarily expressed in the mitochondria and can be found in various tissues including heart liver placenta and kidneys.
- Biological function summary
MCL1 protein functions to maintain cell viability by counteracting pro-apoptotic stimuli. It interacts within the cell as part of a complex network involving other BCL-2 family proteins. MCL1 binds and sequesters BH3-only proteins and Bax therefore preventing them from initiating the apoptotic cascade. This interaction helps cells survive under stress conditions by temporarily inhibiting apoptosis.
- Pathways
MCL1 plays a significant role in the intrinsic (mitochondrial) apoptosis pathway and the cell signaling pathways that facilitate cell survival and proliferation. In the mitochondrial pathway it works closely with other BCL-2 family members like BCL-xL and BCL-2 itself to control the permeability of the mitochondrial outer membrane and thereby influence cytochrome c release. The PI3K/AKT pathway can also regulate MCL1 leading to increases in its expression and stability which promotes cell survival.
- Associated diseases and disorders
Researchers have linked alterations in MCL1 expression to various cancers including leukemia and lymphoma. Overexpression of MCL1 can contribute to chemoresistance in cancer cells by preventing apoptosis. This protein's function is also connected to cardiomyopathies where altered MCL1 expression in cardiac tissue can influence cell death and survival outcomes. BCL-2 and BCL-xL closely related proteins also implicate in these conditions highlighting the central role of the apoptotic regulatory network in disease development.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human MCL1 protein (ab131682)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com