Recombinant Human MCL1 protein (His tag N-Terminus)
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(2 Publications)
Recombinant Human MCL1 protein (His tag N-Terminus) is a Human Fragment protein, in the 1 to 327 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
View Alternative Names
BCL2L3, MCL1, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT, Bcl2-L-3
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human MCL1 protein (His tag N-Terminus) (AB131682)
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MCL1 protein functions to maintain cell viability by counteracting pro-apoptotic stimuli. It interacts within the cell as part of a complex network involving other BCL-2 family proteins. MCL1 binds and sequesters BH3-only proteins and Bax therefore preventing them from initiating the apoptotic cascade. This interaction helps cells survive under stress conditions by temporarily inhibiting apoptosis.
Pathways
MCL1 plays a significant role in the intrinsic (mitochondrial) apoptosis pathway and the cell signaling pathways that facilitate cell survival and proliferation. In the mitochondrial pathway it works closely with other BCL-2 family members like BCL-xL and BCL-2 itself to control the permeability of the mitochondrial outer membrane and thereby influence cytochrome c release. The PI3K/AKT pathway can also regulate MCL1 leading to increases in its expression and stability which promotes cell survival.
Specifications
Form
Liquid
Additional notes
ab131682 was purified using conventional chromatography techniques.
General info
Function
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Sequence similarities
Belongs to the Bcl-2 family.
Post-translational modifications
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.. Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.. Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.. Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159 (PubMed:16543145). Deubiquitinated by USP20; leading to increased stability (PubMed:35063767).
Subcellular localisation
Mitochondrion
Target data
Publications (2)
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Journal of medicinal chemistry 61:2962-2972 PubMed29584430
2018
Applications
Unspecified application
Species
Unspecified reactive species
Oncotarget 7:78958-78970 PubMed27738316
2016
Applications
IP, WB
Species
Human, Human
Complementary Products
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