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AB270081

Recombinant human MCP1 protein (Active)

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Recombinant human MCP1 protein (Active) is a Human Full Length protein, in the 24 to 99 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for sELISA, SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

View Alternative Names

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1

5 Images
Functional Studies - Recombinant human MCP1 protein (Active) (AB270081)
  • FuncS

Supplier Data

Functional Studies - Recombinant human MCP1 protein (Active) (AB270081)

Fully biologically active determined by the dose dependent migration of human PBMCs or THP-1 monocytes.

ED50 is ≤10-50 ng/ml, corresponding to a specific activity of 0.1-0.02 x 106 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3368303-1

Mass Spectrometry - Recombinant human MCP1 protein (Active) (AB270081)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human MCP1 protein (Active) (AB270081)

Mass determination by ESI TOF.

Predicted mass is 8742.08 Da (+/- 10 Da by ESI TOF). Observed mass is 8742 Da.

Sandwich ELISA - Recombinant human MCP1 protein (Active) (AB270081)
  • sELISA

Lab

Sandwich ELISA - Recombinant human MCP1 protein (Active) (AB270081)

Background subtracted standard curve using Human MCP1 Antibody Pair - BSA and Azide free (ab241803) and Recombinant human MCP1 protein (Active) (ab270081) in sandwich ELISA.
The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.

HPLC - Recombinant human MCP1 protein (Active) (AB270081)
  • HPLC

Supplier Data

HPLC - Recombinant human MCP1 protein (Active) (AB270081)

HPLC analysis of ab270081.

SDS-PAGE - Recombinant human MCP1 protein (Active) (AB270081)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human MCP1 protein (Active) (AB270081)

SDS-PAGE analysis of ab270081.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

HPLC, sELISA, Mass Spec, Cell Culture, SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent migration of human PBMCs or THP-1 monocytes.

ED50 is ≤10-50 ng/ml, corresponding to a specific activity of 0.1-0.02 x 106 units/mg.

Accession

P13500

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT","proteinLength":"Full Length","predictedMolecularWeight":"8.74 kDa","actualMolecularWeight":"8.74 kDa","aminoAcidEnd":99,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P13500","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
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Specifications

Form

Lyophilized

Additional notes

=95%  by HPLC

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General info

Function

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Post-translational modifications

Processing at the N-terminus can regulate receptor and target cell selectivity (PubMed:8627182). Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant (PubMed:8627182).. N-Glycosylated.

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Product protocols

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Target data

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).
See full target information CCL2
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