Recombinant Human MD1 protein (denatured) is a Human Full Length protein, in the 21 to 162 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S G G G G K A W P T H V V C S D S G L E V L Y Q S C D P L Q D F G F S V E K C S K Q L K S N I N I R F G I I L R E D I K E L F L D L A L M S Q G S S V L N F S Y P I C E A A L P K F S F C G R R K G E Q I Y Y A G P V N N P E F T I P Q G E Y Q V L L E L Y T E K R S T V A C A N A T I M C S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression (By similarity).
Alternative names
MD1, LY86, Lymphocyte antigen 86, Ly-86, Protein MD-1
Recommended products
Recombinant Human MD1 protein (denatured) is a Human Full Length protein, in the 21 to 162 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O95711-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S G G G G K A W P T H V V C S D S G L E V L Y Q S C D P L Q D F G F S V E K C S K Q L K S N I N I R F G I I L R E D I K E L F L D L A L M S Q G S S V L N F S Y P I C E A A L P K F S F C G R R K G E Q I Y Y A G P V N N P E F T I P Q G E Y Q V L L E L Y T E K R S T V A C A N A T I M C S
- Accession
- O95711
- Protein length
- Full Length
- Predicted molecular weight
- 18.1 kDa
- Amino acids
- 21 to 162
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
May cooperate with CD180 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) and cytokine production. Important for efficient CD180 cell surface expression (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MD1 also known as myeloid differentiation protein 1 is a small glycoprotein with a molecular mass of approximately 20 kDa. It is expressed in a variety of tissues including the spleen liver and peripheral blood leukocytes. MD1 mainly associates with the cell surface linked to its interaction with other membrane proteins. As a protein MD1 plays an important role as an accessory molecule in immune response and homeostasis.
- Biological function summary
MD1 acts as an essential component of the innate immune system. It forms a heterodimeric complex with radioprotective 105 (RP105) another membrane protein. This complex is important for the modulation of Toll-like receptor 4 (TLR4) signaling pathways. By interacting with RP105 MD1 influences the recognition of pathogen-associated molecules and helps regulate inflammatory responses. This involvement is key to controlling immune cell activation.
- Pathways
The TLR4 signaling pathway prominently involves MD1. This pathway is significant in detecting lipopolysaccharides (LPS) from Gram-negative bacteria. The interaction of MD1 with RP105 helps modulate this pathway adjusting the immune response to maintain balance. Additionally MD1 impacts the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway another critical route in inflammation and immune regulation. Its activity within these pathways connects it to proteins like TLR4 and MyD88.
- Associated diseases and disorders
MD1 has a notable connection with autoimmune conditions and infectious diseases. In autoimmune diseases aberrations in MD1 expression or function may lead to dysregulation of immune responses contributing to pathology. For infectious diseases like sepsis MD1’s role in the TLR4 signaling pathway can affect susceptibility and severity through its modulation of immune response. Through these pathways MD1 maintains connections with proteins such as RP105 and TLR4 highlighting its importance in disease progression and immune system dynamics.
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1 product image
SDS-PAGE - Recombinant Human MD1 protein (denatured) (ab167887)
15% SDS-PAGE analysis of ab167887 (3µg).
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