Recombinant Human MDA5 protein (Tagged) is a Human Fragment protein, in the 700 to 1025 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
K L T K L R N T I M E Q Y T R T E E S A R G I I F T K T R Q S A Y A L S Q W I T E N E K F A E V G V K A H H L I G A G H S S E F K P M T Q N E Q K E V I S K F R T G K I N L L I A T T V A E E G L D I K E C N I V I R Y G L V T N E I A M V Q A R G R A R A D E S T Y V L V A H S G S G V I E H E T V N D F R E K M M Y K A I H C V Q N M K P E E Y A H K I L E L Q M Q S I M E K K M K T K R N I A K H Y K N N P S L I T F L C K N C S V L A C S G E D I H V I E K M H H V N M T P E F K E L Y I V R E N K A L Q K K C A D Y Q I N G E I I C K C G Q A W G T M M V H K G L D L P C L K I R N F V V V F K N N S T K K Q Y K K W V E L P I T F P N L D Y S E C C L F S D E D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines (PubMed:28594402, PubMed:32169843, PubMed:33727702). Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length) (PubMed:22160685). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus (PubMed:28606988). Detects coronavirus SARS-CoV-2 (PubMed:33440148, PubMed:33514628). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.
Alternative names
MDA5, RH116, IFIH1, Interferon-induced helicase C domain-containing protein 1, Clinically amyopathic dermatomyositis autoantigen 140 kDa, Helicase with 2 CARD domains, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RIG-I-like receptor 2, RNA helicase-DEAD box protein 116, CADM-140 autoantigen, Helicard, MDA-5, RLR-2
Recommended products
Recombinant Human MDA5 protein (Tagged) is a Human Fragment protein, in the 700 to 1025 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9BYX4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- K L T K L R N T I M E Q Y T R T E E S A R G I I F T K T R Q S A Y A L S Q W I T E N E K F A E V G V K A H H L I G A G H S S E F K P M T Q N E Q K E V I S K F R T G K I N L L I A T T V A E E G L D I K E C N I V I R Y G L V T N E I A M V Q A R G R A R A D E S T Y V L V A H S G S G V I E H E T V N D F R E K M M Y K A I H C V Q N M K P E E Y A H K I L E L Q M Q S I M E K K M K T K R N I A K H Y K N N P S L I T F L C K N C S V L A C S G E D I H V I E K M H H V N M T P E F K E L Y I V R E N K A L Q K K C A D Y Q I N G E I I C K C G Q A W G T M M V H K G L D L P C L K I R N F V V V F K N N S T K K Q Y K K W V E L P I T F P N L D Y S E C C L F S D E D
- Accession
- Q9BYX4
- Protein length
- Fragment
- Predicted molecular weight
- 53.5 kDa
- Amino acids
- 700 to 1025
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines (PubMed:28594402, PubMed:32169843, PubMed:33727702). Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length) (PubMed:22160685). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus (PubMed:28606988). Detects coronavirus SARS-CoV-2 (PubMed:33440148, PubMed:33514628). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.
- Sequence similarities
Belongs to the helicase family. RLR subfamily.
- Post-translational modifications
Sumoylated. Sumoylation positively regulates its role in type I interferon induction and is enhanced by PIAS2-beta.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MDA5 also known as melanoma differentiation-associated protein 5 or IFIH1 is a cytosolic sensor involved in the detection of viral RNA. It is a member of the RIG-I-like receptor family and possesses a molecular weight of approximately 116 kilodaltons. MDA5 protein is expressed across various tissues but shows higher expression in the immune system's cells including macrophages and dendritic cells. MDA5 recognizes long double-stranded RNA a common viral replication byproduct triggering immune responses.
- Biological function summary
In cellular defense mechanisms MDA5 plays an important role in initiating antiviral responses. It operates as part of a signaling complex that includes MAVS (mitochondrial antiviral-signaling protein) leading to the production of type I interferons and pro-inflammatory cytokines. These responses promote an antiviral state in host cells aiding in the containment and clearance of viral infections. MDA5 antibodies can be useful tools for studying these immune processes.
- Pathways
MDA5 interacts with antiviral signaling pathways such as the IFN signaling pathway and the RIG-I-like receptor signaling pathway. Upon recognizing viral RNA MDA-5 cooperates with proteins like RIG-I and MAVS to activate downstream signaling cascades that mediate immune responses. This collaboration enables efficient viral recognition and response limiting viral replication and spread.
- Associated diseases and disorders
MDA5 has associations with autoimmune diseases like systemic lupus erythematosus and certain types of dermatomyositis. These conditions often involve autoantibodies against MDA5 indicating a possible role in pathogenesis. In these diseases altered function or regulation of related proteins including IFN-induced proteins may contribute to chronic inflammation and autoimmunity. Understanding MDA5's involvement in these contexts is essential for developing therapeutic strategies.
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1 product image
SDS-PAGE - Recombinant Human MDA5 protein (Tagged) (ab240773)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab240773.
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