Recombinant human MDH2 protein is a Human Full Length protein, in the 25 to 338 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS, FuncS.
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- Nature chemical biology 13:494-5002017L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew M Intlekofer et. al.PubMed 28263965
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS, FuncS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A K V A V L G A S G G I G Q P L S L L L K N S P L V S R L T L Y D I A H T P G V A A D L S H I E T K A A V K G Y L G P E Q L P D C L K G C D V V V I P A G V P R K P G M T R D D L F N T N A T I V A T L T A A C A Q H C P E A M I C V I A N P V N S T I P I T A E V F K K H G V Y N P N K I F G V T T L D I V R A N T F V A E L K G L D P A R V N V P V I G G H A G K T I I P L I S Q C T P K V D F P Q D Q L T A L T G R I Q E A G T E V V K A K A G A G S A T L S M A Y A G A R F V F S L V D A M N G K E G V V E C S F V K S Q E T E C T Y F S T P L L L G K K G I E K N L G I G K V S S F E E K M I S D A I P E L K A S I K K G E D F V K T L K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Alternative names
MDH2
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Recombinant human MDH2 protein is a Human Full Length protein, in the 25 to 338 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 30 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25°C
Activity Assay
- Prepare assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 100 mM Potassium phosphate, 0.13mM beta-nicotinamide adenine dinucleotide, reduced form, 0.25mM oxalacetic acid.
- Add recombinant MDH protein with various concentrations (0.1ug, 0.5ug, 1ug) in assay buffer.
- Mix by inversion and equilibrate to 1°C and monitor at A340nm until the value is constant using a spectrophotometer.
- Record the increase at A340nm for approximately 2 minutes
- Accession
- P40926-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A K V A V L G A S G G I G Q P L S L L L K N S P L V S R L T L Y D I A H T P G V A A D L S H I E T K A A V K G Y L G P E Q L P D C L K G C D V V V I P A G V P R K P G M T R D D L F N T N A T I V A T L T A A C A Q H C P E A M I C V I A N P V N S T I P I T A E V F K K H G V Y N P N K I F G V T T L D I V R A N T F V A E L K G L D P A R V N V P V I G G H A G K T I I P L I S Q C T P K V D F P Q D Q L T A L T G R I Q E A G T E V V K A K A G A G S A T L S M A Y A G A R F V F S L V D A M N G K E G V V E C S F V K S Q E T E C T Y F S T P L L L G K K G I E K N L G I G K V S S F E E K M I S D A I P E L K A S I K K G E D F V K T L K
- Accession
- P40926
- Protein length
- Full Length
- Predicted molecular weight
- 35.2 kDa
- Amino acids
- 25 to 338
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab99238 is purified using conventional chromatography techniques.
General info
- Sequence similarities
Belongs to the LDH/MDH superfamily. MDH type 1 family.
- Post-translational modifications
Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation by about 60%.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MDH2 also known as malate dehydrogenase 2 or mitochondrial malate dehydrogenase is an enzyme with a molecular mass of approximately 35 kDa. This enzyme catalyzes the conversion of malate to oxaloacetate using NAD+ as a cofactor. It is predominantly expressed in the mitochondria where it plays an important role in cellular respiration. The enzyme enables the malate dehydrogenase reaction which is key for the functioning of the tricarboxylic acid cycle.
- Biological function summary
MDH2 participates in the critical process of energy production within the cell. While it does not form a complex itself its activity is intimately connected with other enzymes in mitochondrial energy metabolism. The malate dehydrogenase assay often measures the activity of MDH2 to understand the metabolic status of cells. By facilitating the oxidation of malate MDH2 aids in maintaining the efficiency of the mitochondrial electron transport chain by regenerating NADH.
- Pathways
The enzyme is essential in the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle MDH2 collaborates with enzymes like citrate synthase and isocitrate dehydrogenase to assist in the conversion of acetyl-CoA into energy-rich molecules. The malate-aspartate shuttle on the other hand involves MDH2 working closely with aspartate transaminase to transfer reducing equivalents into the mitochondria. These pathways highlight MDH2’s importance in cellular energy homeostasis.
- Associated diseases and disorders
Mutations or dysregulation in MDH2 have connections to certain metabolic conditions and cancers. For example alterations in MDH2 activity might contribute to conditions like mitochondrial myopathy altering energy metabolism. Moreover MDH2 is associated with NADH-producing enzymes whose dysregulation can support oncogenic pathways in cancer. Understanding these associations helps researchers pursue therapeutic targets that modulate MDH2 activity.
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1 product image
SDS-PAGE - Recombinant human MDH2 protein (ab99238)
15% SDS-PAGE showing ab99238 at approximately 35.2kDa (3μg).
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