JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB99238

Recombinant human MDH2 protein

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant human MDH2 protein is a Human Full Length protein, in the 25 to 338 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec, FuncS.

View Alternative Names

MDH2

1 Images
SDS-PAGE - Recombinant human MDH2 protein (AB99238)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MDH2 protein (AB99238)

15% SDS-PAGE showing ab99238 at approximately 35.2kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE, Mass Spec

applications

Biologically active

Yes

Biological activity

Specific activity is > 30 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25°C

Activity Assay

  1. Prepare assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 100 mM Potassium phosphate, 0.13mM beta-nicotinamide adenine dinucleotide, reduced form, 0.25mM oxalacetic acid.
  2. Add recombinant MDH protein with various concentrations (0.1ug, 0.5ug, 1ug) in assay buffer.
  3. Mix by inversion and equilibrate to 1°C and monitor at A340nm until the value is constant using a spectrophotometer.
  4. Record the increase at A340nm for approximately 2 minutes
Accession

P40926

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAKVAVLGASGGIGQPLSLLLKNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGVPRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGVYNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDFPQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECSFVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFVKTLK","proteinLength":"Full Length","predictedMolecularWeight":"35.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":338,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P40926","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MDH2 also known as malate dehydrogenase 2 or mitochondrial malate dehydrogenase is an enzyme with a molecular mass of approximately 35 kDa. This enzyme catalyzes the conversion of malate to oxaloacetate using NAD+ as a cofactor. It is predominantly expressed in the mitochondria where it plays an important role in cellular respiration. The enzyme enables the malate dehydrogenase reaction which is key for the functioning of the tricarboxylic acid cycle.
Biological function summary

MDH2 participates in the critical process of energy production within the cell. While it does not form a complex itself its activity is intimately connected with other enzymes in mitochondrial energy metabolism. The malate dehydrogenase assay often measures the activity of MDH2 to understand the metabolic status of cells. By facilitating the oxidation of malate MDH2 aids in maintaining the efficiency of the mitochondrial electron transport chain by regenerating NADH.

Pathways

The enzyme is essential in the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle MDH2 collaborates with enzymes like citrate synthase and isocitrate dehydrogenase to assist in the conversion of acetyl-CoA into energy-rich molecules. The malate-aspartate shuttle on the other hand involves MDH2 working closely with aspartate transaminase to transfer reducing equivalents into the mitochondria. These pathways highlight MDH2’s importance in cellular energy homeostasis.

Mutations or dysregulation in MDH2 have connections to certain metabolic conditions and cancers. For example alterations in MDH2 activity might contribute to conditions like mitochondrial myopathy altering energy metabolism. Moreover MDH2 is associated with NADH-producing enzymes whose dysregulation can support oncogenic pathways in cancer. Understanding these associations helps researchers pursue therapeutic targets that modulate MDH2 activity.
style
left-column

Specifications

Form

Liquid

Additional notes

ab99238 is purified using conventional chromatography techniques.

style
left-column

General info

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Post-translational modifications

Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation by about 60%.

Subcellular localisation

Mitochondrion matrix

style
left-column

Product protocols

style
left-column

Target data

See full target information MDH2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature chemical biology 13:494-500 PubMed28263965

2017

L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew M Intlekofer,Bo Wang,Hui Liu,Hardik Shah,Carlos Carmona-Fontaine,Ariën S Rustenburg,Salah Salah,M R Gunner,John D Chodera,Justin R Cross,Craig B Thompson
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB113858

Recombinant human IDH1 protein

SDS-PAGE - Recombinant human IDH1 protein (AB113858)

  • 0
  • 0
Proteins & Peptides

AB99244

Recombinant Human MDH1 protein

SDS-PAGE - Recombinant Human MDH1 protein (AB99244)

  • 0
  • 0
Proteins & Peptides

AB318258

Recombinant human PKM2 protein (His tag)

Mass Spectrometry - Recombinant human PKM2 protein (His tag) (AB318258)

  • 0
  • 0
Assay Kits

AB119693

Malate Dehydrogenase 2 (MDH2) Activity Assay

ELISA - Malate Dehydrogenase 2 (MDH2) Activity Assay (AB119693)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com