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AB152518

Recombinant Human ME1 protein (GST tag N-Terminus)

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Recombinant Human ME1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 572 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

NADP-dependent malic enzyme, NADP-ME, Malic enzyme 1, ME1

1 Images
SDS-PAGE - Recombinant Human ME1 protein (GST tag N-Terminus) (AB152518)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ME1 protein (GST tag N-Terminus) (AB152518)

12.5% SDS-PAGE analysis of ab152518 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P48163

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB97445

Anti-ME1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ME1 antibody (AB97445)

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  • 1
Proteins & Peptides

AB238230

Recombinant Human ME3 protein (His tag)

SDS-PAGE - Recombinant Human ME3 protein (His tag) (AB238230)

  • 0
  • 0
Proteins & Peptides

AB201360

Recombinant Human ME2 protein

  • 0
  • 0
Proteins & Peptides

AB152558

Recombinant Human NDUFB8 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human NDUFB8 protein (GST tag N-Terminus) (AB152558)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MEPEAPRRRHTHQRGYLLTRNPHLNKDLAFTLEERQQLNIHGLLPPSFNSQEIQVLRVVKNFEHLNSDFDRYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHDRGHIASVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQECLPVILDVGTENEELLKDPLYIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQGAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGRASLTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQCYKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLTTAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATVYPEPQNKEAFVRSQMYSTDYDQILPDCYSWPEEVQKIQTKVDQ","proteinLength":"Full Length","predictedMolecularWeight":"88.66 kDa","actualMolecularWeight":null,"aminoAcidEnd":572,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P48163","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ME1 also known as malic enzyme 1 is a cytosolic enzyme that catalyzes the reversible oxidative decarboxylation of malate to pyruvate generating NADPH in the process. This enzyme contributes to cellular lipid and cholesterol biosynthesis. ME1 has an approximate molecular weight of 64 kDa. You can find ME1 expression mainly in tissues with high biosynthetic activities such as liver fat and kidney.
Biological function summary

ME1 plays an important role in regulating the cellular redox state and maintaining metabolic homeostasis. It supplies NADPH which is necessary for fatty acid synthesis and lipid metabolism supporting anabolic processes in cells. ME1 works as a part of a metabolic enzyme complex that coordinates with other enzymes to produce reducing equivalents required for detoxification and biosynthesis. Scientists frequently study its role in lipid synthesis due to its implications in metabolic disorders.

Pathways

ME1 is involved in lipid metabolism and the pentose phosphate pathway where it helps generate reducing power. ME1 interacts with other enzymes like glucose-6-phosphate dehydrogenase which also provides NADPH showing its interconnected role in maintaining cellular redox balance. ME1’s activity supports both pathways by contributing to the production of ribose-5-phosphate and other precursors important for nucleotide biosynthesis and antioxidant defense.

ME1 has links to obesity and cancer where its dysregulation can promote tumorigenesis and metabolic disorders. Studies suggest aberrant ME1 expression may lead to altered lipid biosynthesis contributing to excessive fat accumulation in obesity. ME1 and glutamine synthetase a protein connected in cancer metabolism work together to support cell proliferation through their metabolic pathways highlighting ME1’s potential as a target in metabolic and cancer therapy.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP(+) and divalent metal ions, and decarboxylation of oxaloacetate.

Sequence similarities

Belongs to the malic enzymes family.

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Product protocols

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Target data

Catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP(+) and divalent metal ions, and decarboxylation of oxaloacetate.
See full target information ME1
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Product promise

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