JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB238230

Recombinant Human ME3 protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human ME3 protein (His tag) is a Human Fragment protein, in the 24 to 590 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

NADP-ME, Malic enzyme 3, ME3

1 Images
SDS-PAGE - Recombinant Human ME3 protein (His tag) (AB238230)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ME3 protein (His tag) (AB238230)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab238230.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q16798

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB172972

Anti-ME3 antibody [EPR10378]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME3 antibody [EPR10378] (AB172972)

  • 4
  • 1
Proteins & Peptides

AB163782

Recombinant Human VISTA protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human VISTA protein (GST tag N-Terminus) (AB163782)

  • 0
  • 0
Proteins & Peptides

AB216228

Recombinant human B7H4 protein (Fc Chimera Active)

Bioactive

  • 0
  • 0
Proteins & Peptides

AB198464

Recombinant human HAUSP / USP7 protein (His-DDDDK tag N-Terminus)

Functional Studies - Recombinant human HAUSP / USP7 protein (His-DDDDK tag N-Terminus) (AB198464)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"linker":null,"sequence":"RWTPTAPAQGCHSKPGPARPVPLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGKTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDYAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLD","proteinLength":"Fragment","predictedMolecularWeight":"67.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":590,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q16798","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Form
Liquid
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ME3 also known as malic enzyme 3 or mitochondrial NADP+-dependent malic enzyme plays an important role in cellular metabolism. It catalyzes the oxidative decarboxylation of malate to pyruvate generating NADPH in the process. The protein has a molecular mass of approximately 65 kDa. ME3 is mainly expressed in tissues with high metabolic demands such as the liver and adipose tissue where it supports various biosynthetic pathways.
Biological function summary

ME3 influences cellular redox balance via its production of NADPH an important reducing agent. It is not part of a larger protein complex but serves an essential function on its own. ME3 contributes to lipid biosynthesis by supplying NADPH for fatty acid elongation and cholesterol synthesis which supports cell growth and proliferation. Its activity impacts overall metabolic homeostasis.

Pathways

ME3 has a significant role in the tricarboxylic acid (TCA) cycle and lipid metabolism. It interfaces with the TCA cycle by providing a critical link between carbohydrate and lipid metabolism through pyruvate production. ME3 interacts with other metabolic enzymes like citrate synthase emphasizing its integrating function in energy production and storage pathways.

ME3's dysregulation associates with metabolic conditions such as obesity and non-alcoholic fatty liver disease (NAFLD). Altered ME3 activity can impact fatty acid metabolism and contribute to lipid accumulation in liver cells. It also interacts with proteins like acetyl-CoA carboxylase involved in fatty acid biosynthesis highlighting its potential role in disease progression. Understanding ME3's function offers insights into the etiology of these metabolic disorders and opportunities for therapeutic intervention.
style
left-column

General info

Function

Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+) as a cofactor (PubMed : 7818469). Can also reverse the decarboxylation reaction, but only with significantly lower efficiency (PubMed : 7818469).

Sequence similarities

Belongs to the malic enzymes family.

Subcellular localisation

Mitochondrion matrix

style
left-column

Product protocols

style
left-column

Target data

Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+) as a cofactor (PubMed : 7818469). Can also reverse the decarboxylation reaction, but only with significantly lower efficiency (PubMed : 7818469).
See full target information ME3
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com