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AB196070

Recombinant Human MeCP2 protein (His tag)

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Recombinant Human MeCP2 protein (His tag) is a Human Full Length protein, in the 1 to 486 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2

1 Images
SDS-PAGE - Recombinant Human MeCP2 protein (His tag) (AB196070)
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Supplier Data

SDS-PAGE - Recombinant Human MeCP2 protein (His tag) (AB196070)

SDS-PAGE - Recombinant Human MeCP2 protein (His tag) (ab196070).

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, HPLC

applications

Biologically active

No

Accession

P51608

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 8 Constituents: 99% Tris, 0.87% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MVAGMLGLREEKSEDQDLQGLKDKPLKFKKVKKDKKEEKEGKHEPVQPSAHHSAEPAEAGKAETSEGSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVSVDHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"53.48 kDa","actualMolecularWeight":null,"aminoAcidEnd":486,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P51608","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
A few weeks
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Lyophilized

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General info

Function

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).

Post-translational modifications

Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
See full target information MECP2
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Complementary Products

Primary Antibodies

AB253197

Anti-MeCP2 antibody [EPR23201-3]

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Immunocytochemistry/ Immunofluorescence - Anti-MeCP2 antibody [EPR23201-3] (AB253197)

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Cell Lines & Lysates

AB94140

MeCP2 overexpression 293T lysate (whole cell)

Western blot - MeCP2 overexpression 293T lysate (whole cell) (AB94140)

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Proteins & Peptides

AB236335

Recombinant Human PU.1/Spi1 protein (His tag)

Mass Spectrometry - Recombinant Human PU.1/Spi1 protein (His tag) (AB236335)

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Primary Antibodies

AB177483

Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998]

RabMAb|Recombinant

Western blot - Anti-GABA Transporter 1 / GAT 1 antibody [EPR12998] (AB177483)

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