Recombinant Human MECR protein is a Human Full Length protein, in the 54 to 373 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M P A K V V E L K N L E L A A V R G S D V R V K M L A A P I N P S D I N M I Q G N Y G L L P E L P A V G G N E G V A Q V V A V G S N V T G L K P G D W V I P A N A G L G T W R T E A V F S E E A L I Q V P S D I P L Q S A A T L G V N P C T A Y R M L M D F E Q L Q P G D S V I Q N A S N S G V G Q A V I Q I A A A L G L R T I N V V R D R P D I Q K L S D R L K S L G A E H V I T E E E L R R P E M K N F F K D M P Q P R L A L N C V G G K S S T E L L R Q L A R G G T M V T Y G G M A K Q P V V A S V S L L I F K D L K L R G F W L S Q W K K D H S P D Q F K E L I L T L C D L I R R G Q L T A P A C S Q V P L Q D Y Q S A L E A S M K P F I S S K Q I L T M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates (PubMed:12654921, PubMed:18479707, PubMed:27817865). May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells (By similarity). Involved in iron homeostasis; affecting Fe-S cluster assembly and ceramide metabolism (PubMed:37653044). Required for proper morphology and bioenergetic functions of mitochondria (PubMed:37653044). Required for maintenance of neurons (By similarity).
Alternative names
NBRF1, CGI-63, MECR, 2-enoyl thioester reductase, Nuclear receptor-binding factor 1, HsNrbf-1, NRBF-1
Recommended products
Recombinant Human MECR protein is a Human Full Length protein, in the 54 to 373 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9BV79-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M P A K V V E L K N L E L A A V R G S D V R V K M L A A P I N P S D I N M I Q G N Y G L L P E L P A V G G N E G V A Q V V A V G S N V T G L K P G D W V I P A N A G L G T W R T E A V F S E E A L I Q V P S D I P L Q S A A T L G V N P C T A Y R M L M D F E Q L Q P G D S V I Q N A S N S G V G Q A V I Q I A A A L G L R T I N V V R D R P D I Q K L S D R L K S L G A E H V I T E E E L R R P E M K N F F K D M P Q P R L A L N C V G G K S S T E L L R Q L A R G G T M V T Y G G M A K Q P V V A S V S L L I F K D L K L R G F W L S Q W K K D H S P D Q F K E L I L T L C D L I R R G Q L T A P A C S Q V P L Q D Y Q S A L E A S M K P F I S S K Q I L T M
- Accession
- Q9BV79
- Protein length
- Full Length
- Predicted molecular weight
- 36.9 kDa
- Amino acids
- 54 to 373
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab99325 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates (PubMed:12654921, PubMed:18479707, PubMed:27817865). May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells (By similarity). Involved in iron homeostasis; affecting Fe-S cluster assembly and ceramide metabolism (PubMed:37653044). Required for proper morphology and bioenergetic functions of mitochondria (PubMed:37653044). Required for maintenance of neurons (By similarity).
- Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
- Subcellular localisation
- Mitochondrion, Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MECR or mitochondrial trans-2-enoyl-CoA reductase plays an important role in mitochondrial fatty acid synthesis. This enzyme is involved in the final reduction step of trans-2-enoyl-CoA to acyl-CoA. Known alternately as NRBF1 MECR has a molecular mass of approximately 37 kDa. It finds expression primarily in mitochondria-rich tissues like heart liver and skeletal muscle where energy production and fatty acid metabolism are highly active.
- Biological function summary
The MECR protein contributes to the biosynthesis of mitochondrial fatty acids by catalyzing the reduction of trans-2-enoyl-CoA. It operates as part of a multicomponent enzyme complex involved in mitochondrial fatty acid synthesis. This process is necessary for maintaining proper mitochondrial membrane integrity and function affecting multiple cellular functions beyond lipid metabolism including regulation of energy homeostasis.
- Pathways
MECR integrates mainly into mitochondrial fatty acid synthesis pathways. It collaborates with other enzymes to regulate fatty acid metabolism contributing to energy production within mitochondria. MECR partners with the protein ETFDH which transfers electrons necessary for the enzymatic reactions within the pathway. Further it interacts indirectly with the beta-oxidation pathway which breaks down fatty acids into acyl-CoA units subsequently entering the mitochondrial electron transport chain for ATP production.
- Associated diseases and disorders
Defective MECR activity has been linked to progressive neurological disorders and cardiomyopathy. Disorders like mitochondrial myopathy are associated with mutations affecting MECR's enzymatic efficiency. Other proteins such as ACAD9 connect with MECR where both participate in similar or adjacent pathways disrupted in these disorders. This link emphasizes the potential role of MECR in therapeutic strategies targeting mitochondrial dysfunction-related diseases.
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1 product image
SDS-PAGE - Recombinant Human MECR protein (ab99325)
15% SDS-PAGE showing ab99325 (3μg).
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