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AB99325

Recombinant Human MECR protein

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Recombinant Human MECR protein is a Human Full Length protein, in the 54 to 373 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

NBRF1, CGI-63, MECR, 2-enoyl thioester reductase, Nuclear receptor-binding factor 1, HsNrbf-1, NRBF-1

1 Images
SDS-PAGE - Recombinant Human MECR protein (AB99325)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MECR protein (AB99325)

15% SDS-PAGE showing ab99325 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9BV79

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMPAKVVELKNLELAAVRGSDVRVKMLAAPINPSDINMIQGNYGLLPELPAVGGNEGVAQVVAVGSNVTGLKPGDWVIPANAGLGTWRTEAVFSEEALIQVPSDIPLQSAATLGVNPCTAYRMLMDFEQLQPGDSVIQNASNSGVGQAVIQIAAALGLRTINVVRDRPDIQKLSDRLKSLGAEHVITEEELRRPEMKNFFKDMPQPRLALNCVGGKSSTELLRQLARGGTMVTYGGMAKQPVVASVSLLIFKDLKLRGFWLSQWKKDHSPDQFKELILTLCDLIRRGQLTAPACSQVPLQDYQSALEASMKPFISSKQILTM","proteinLength":"Full Length","predictedMolecularWeight":"36.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":373,"aminoAcidStart":54,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9BV79","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MECR or mitochondrial trans-2-enoyl-CoA reductase plays an important role in mitochondrial fatty acid synthesis. This enzyme is involved in the final reduction step of trans-2-enoyl-CoA to acyl-CoA. Known alternately as NRBF1 MECR has a molecular mass of approximately 37 kDa. It finds expression primarily in mitochondria-rich tissues like heart liver and skeletal muscle where energy production and fatty acid metabolism are highly active.
Biological function summary

The MECR protein contributes to the biosynthesis of mitochondrial fatty acids by catalyzing the reduction of trans-2-enoyl-CoA. It operates as part of a multicomponent enzyme complex involved in mitochondrial fatty acid synthesis. This process is necessary for maintaining proper mitochondrial membrane integrity and function affecting multiple cellular functions beyond lipid metabolism including regulation of energy homeostasis.

Pathways

MECR integrates mainly into mitochondrial fatty acid synthesis pathways. It collaborates with other enzymes to regulate fatty acid metabolism contributing to energy production within mitochondria. MECR partners with the protein ETFDH which transfers electrons necessary for the enzymatic reactions within the pathway. Further it interacts indirectly with the beta-oxidation pathway which breaks down fatty acids into acyl-CoA units subsequently entering the mitochondrial electron transport chain for ATP production.

Defective MECR activity has been linked to progressive neurological disorders and cardiomyopathy. Disorders like mitochondrial myopathy are associated with mutations affecting MECR's enzymatic efficiency. Other proteins such as ACAD9 connect with MECR where both participate in similar or adjacent pathways disrupted in these disorders. This link emphasizes the potential role of MECR in therapeutic strategies targeting mitochondrial dysfunction-related diseases.
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Specifications

Form

Liquid

Additional notes

ab99325 is purified using conventional chromatography techniques.

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General info

Function

Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates (PubMed : 12654921, PubMed : 18479707, PubMed : 27817865). May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells (By similarity). Involved in iron homeostasis; affecting Fe-S cluster assembly and ceramide metabolism (PubMed : 37653044). Required for proper morphology and bioenergetic functions of mitochondria (PubMed : 37653044). Required for maintenance of neurons (By similarity).

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates (PubMed : 12654921, PubMed : 18479707, PubMed : 27817865). May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells (By similarity). Involved in iron homeostasis; affecting Fe-S cluster assembly and ceramide metabolism (PubMed : 37653044). Required for proper morphology and bioenergetic functions of mitochondria (PubMed : 37653044). Required for maintenance of neurons (By similarity).
See full target information MECR
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Complementary Products

Primary Antibodies

AB254707

Anti-MECR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MECR antibody (AB254707)

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