Recombinant Human MED3 protein (denatured) is a Human Full Length protein, in the 1 to 311 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
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- Molecular and cellular biology 39:2019RNA Binding Protein HuR Promotes Autophagosome Formation by Regulating Expression of Autophagy-Related Proteins 5, 12, and 16 in Human Hepatocellular Carcinoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEunbyul Ji et. al.PubMed 30602494
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A D V I N V S V N L E A F S Q A I S A I Q A L R S S V S R V F D C L K D G M R N K E T L E G R E K A F I A H F Q D N L H S V N R D L N E L E R L S N L V G K P S E N H P L H N S G L L S L D P V Q D K T P L Y S Q L L Q A Y K W S N K L Q Y H A G L A S G L L N Q Q S L K R S A N Q M G V S A K R R P K A Q P T T L V L P P Q Y V D D V I S R I D R M F P E M S I H L S R P N G T S A M L L V T L G K V L K V I V V M R S L F I D R T I V K G Y N E N V Y T E D G K L D I W S K S N Y Q V F Q K V T D H A T T A L L H Y Q L P Q M P D V V V R S F M T W L R S Y I K L F Q A P C Q R C G K F L Q D G L P P T W R D F R T L E A F H D T C R Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Alternative names
CRSP34, CRSP8, MED27, Mediator of RNA polymerase II transcription subunit 27, Cofactor required for Sp1 transcriptional activation subunit 8, Mediator complex subunit 27, P37 TRAP/SMCC/PC2 subunit, Transcriptional coactivator CRSP34, CRSP complex subunit 8
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Recombinant Human MED3 protein (denatured) is a Human Full Length protein, in the 1 to 311 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q6P2C8-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A D V I N V S V N L E A F S Q A I S A I Q A L R S S V S R V F D C L K D G M R N K E T L E G R E K A F I A H F Q D N L H S V N R D L N E L E R L S N L V G K P S E N H P L H N S G L L S L D P V Q D K T P L Y S Q L L Q A Y K W S N K L Q Y H A G L A S G L L N Q Q S L K R S A N Q M G V S A K R R P K A Q P T T L V L P P Q Y V D D V I S R I D R M F P E M S I H L S R P N G T S A M L L V T L G K V L K V I V V M R S L F I D R T I V K G Y N E N V Y T E D G K L D I W S K S N Y Q V F Q K V T D H A T T A L L H Y Q L P Q M P D V V V R S F M T W L R S Y I K L F Q A P C Q R C G K F L Q D G L P P T W R D F R T L E A F H D T C R Q
- Accession
- Q6P2C8
- Protein length
- Full Length
- Predicted molecular weight
- 37.8 kDa
- Amino acids
- 1 to 311
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
- Sequence similarities
Belongs to the Mediator complex subunit 27 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as CRSP8
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MED3 protein also known as Vitamin D3 receptor interacting protein complex 12 (DRIP12) possesses a mass of approximately 64 kDa. This protein acts as part of the larger Mediator complex which plays an important role in the regulation of gene transcription. MED3 is ubiquitously expressed in various tissues reflecting its essential function in gene expression. It modulates transcription by serving as a bridge between transcription factors and RNA polymerase II.
- Biological function summary
Functions of MED3 extend to the facilitation of transcriptional activation processes. Being a part of the Mediator complex MED3 influences RNA polymerase II-dependent transcription in eukaryotic cells. It works closely with other core subunits to recruit the complex to gene promoters and enable efficient transcription initiation. MED3 by interacting with transcriptional regulators adjusts the transcriptional output necessary for cell growth and differentiation.
- Pathways
Studies show that MED3 is involved in the regulation of Wnt signaling and the Notch signaling pathways. These pathways are key in cell fate determination and developmental processes. The MED3 protein intersects with the activities of other transcriptional regulators like beta-catenin in the Wnt pathway emphasizing its impact on important developmental gene expression.
- Associated diseases and disorders
MED3 holds an association with certain cancers and developmental disorders. Dysregulation of MED3 activity may contribute to colorectal cancer implicating its involvement with the aberrant activation of Wnt signaling. In some developmental disorders the improper function of MED3 can disrupt normal transcriptional regulation also impacting Notch signaling-related proteins. Understanding MED3's role in these contexts provides insights into its potential as a therapeutic target.
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1 product image
SDS-PAGE - Recombinant Human MED3 protein (denatured) (ab180327)
15% SDS-PAGE analysis of 3μg ab180327.
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