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Recombinant Human MEF2A protein (His tag) is a Human Full Length protein, in the 1 to 499 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, WB, MS.

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Images

Western blot - Recombinant Human MEF2A protein (His tag) (AB204772), expandable thumbnail
  • SDS-PAGE - Recombinant Human MEF2A protein (His tag) (AB204772), expandable thumbnail

Key facts

Purity

>90%

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, WB, MS

Biologically active

No

Amino acid sequence

M G R K K I Q I T R I M D E R N R Q V T F T K R K F G L M K K A Y E L S V L C D C E I A L I I F N S S N K L F Q Y A S T D M D K V L L K Y T E Y N E P H E S R T N S D I V E A L N K K E H R G C D S P D P D T S Y V L T P H T E E K Y K K I N E E F D N M M R N H K I A P G L P P Q N F S M S V T V P V T S P N A L S Y T N P G S S L V S P S L A A S S T L T D S S M L S P P Q T T L H R N V S P G A P Q R P P S T G N A G G M L S T T D L T V P N G A G S S P V G N G F V N S R A S P N L I G A T G A N S L G K V M P T K S P P P P G G G N L G M N S R K P D L R V V I P P S S K G M M P P L N T Q R I S S S Q A T Q P L A T P V V S V T T P S L P P Q G L V Y S A M P T A Y N T D Y S L T S A D L S A L Q G F N S P G M L S L G Q V S A W Q Q H H L G Q A A L S S L V A G G Q L S Q G S N L S I N T N Q N I S I K S E P I S P P R D R M T P S G F Q Q Q Q Q Q Q Q Q Q Q P P P P P Q P Q P Q P P Q P Q P R Q E M G R S P V D S L S S S S S S Y D G S D R E D P R G D F H S P I V L G R P P N T E D R E S P S V K R M R M D A W V T

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.

Alternative names

Recommended products

Recombinant Human MEF2A protein (His tag) is a Human Full Length protein, in the 1 to 499 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, WB, MS.

Key facts

Purity

>90%

Expression system

Escherichia coli

Applications

SDS-PAGE, WB, MS

Accession
Q02078-5
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

Constituents: 100% PBS

Sequence info

Amino acid sequence

M G R K K I Q I T R I M D E R N R Q V T F T K R K F G L M K K A Y E L S V L C D C E I A L I I F N S S N K L F Q Y A S T D M D K V L L K Y T E Y N E P H E S R T N S D I V E A L N K K E H R G C D S P D P D T S Y V L T P H T E E K Y K K I N E E F D N M M R N H K I A P G L P P Q N F S M S V T V P V T S P N A L S Y T N P G S S L V S P S L A A S S T L T D S S M L S P P Q T T L H R N V S P G A P Q R P P S T G N A G G M L S T T D L T V P N G A G S S P V G N G F V N S R A S P N L I G A T G A N S L G K V M P T K S P P P P G G G N L G M N S R K P D L R V V I P P S S K G M M P P L N T Q R I S S S Q A T Q P L A T P V V S V T T P S L P P Q G L V Y S A M P T A Y N T D Y S L T S A D L S A L Q G F N S P G M L S L G Q V S A W Q Q H H L G Q A A L S S L V A G G Q L S Q G S N L S I N T N Q N I S I K S E P I S P P R D R M T P S G F Q Q Q Q Q Q Q Q Q Q Q P P P P P Q P Q P Q P P Q P Q P R Q E M G R S P V D S L S S S S S S Y D G S D R E D P R G D F H S P I V L G R P P N T E D R E S P S V K R M R M D A W V T

Accession

Q02078

Protein length

Full Length

Predicted molecular weight

55 kDa

Amino acids

1 to 499

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

Additional notes

by immobilized-metal affinity chromatography (IMAC) purification.

General info

Function

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.

Sequence similarities

Belongs to the MEF2 family.

Post-translational modifications

Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MEF2A or Myocyte Enhancer Factor 2A is a transcription factor involved in regulating gene expression. MEF2A is approximately 55 kDa in size and is expressed in a variety of tissues including muscle brain and heart. It is part of the MEF2 family of transcription factors which all share a similar MADS-box/MEF2 domain structure. MEF2A binds to DNA and influences the transcription of genes involved in muscle differentiation and development.

Biological function summary

MEF2A participates in transcriptional regulation as part of a larger complex that includes other regulatory proteins. This transcription factor plays an essential role in the development and function of muscles. It activates the transcription of genes necessary for muscle fiber formation and adaptation. MEF2A also influences the expression of genes related to neuronal survival and plasticity impacting cognitive function and memory in the brain.

Pathways

MEF2A is involved in both the MAPK/ERK and PI3K/Akt pathways which are significant for cellular growth survival and differentiation. MEF2A interacts with proteins like MAPK and PI3K impacting these signaling pathways that regulate cell proliferation and apoptosis. This interaction highlights its role in cellular response to external stimuli and stress signals positioning MEF2A as an important factor in cell fate decisions.

Associated diseases and disorders

Mutations or dysregulation of MEF2A are connected to cardiovascular diseases particularly coronary artery disease. MEF2A mutations can disrupt normal transcriptional regulation leading to impaired cardiovascular function. Additionally alterations in MEF2A function are linked to neurodegenerative disorders such as Alzheimer's disease where it may interact with proteins including Tau and amyloid precursor protein influencing neuronal health and survival.

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2 product images

  • Western blot - Recombinant Human MEF2A protein (His tag) (ab204772), expandable thumbnail

    Western blot - Recombinant Human MEF2A protein (His tag) (ab204772)

    All lanes: anti-6xHis monoclonal antibody

    All lanes: Western blot - Recombinant Human MEF2A protein (His tag) (ab204772)

  • SDS-PAGE - Recombinant Human MEF2A protein (His tag) (ab204772), expandable thumbnail

    SDS-PAGE - Recombinant Human MEF2A protein (His tag) (ab204772)

    10% SDS-PAGE analysis of ab204772 stained with Coomassie Blue.

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Product protocols

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