Recombinant Human MEF2D protein is a Human Full Length protein, in the 1 to 514 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M G R K K I Q I Q R I T D E R N R R V T F T K R K F G L M K K A Y E L S V L C D C E I A L I I F N H S N K L F Q Y A S T D M D K V L L K Y T E Y N E P H E S R T N A D I I E T L R K K G F N G C D S P E P D G E D S L E Q S P L L E D K Y R R A S E E L D G L F R R Y G S T V P A P N F A M P V T V P V S N Q S S L Q F S N P S G S L V T P S L V T S S L T D P R L L S P Q Q P A L Q R N S V S P G L P Q R P A S A G A M L G G D L N S A N G A C P S P V G N G Y V S A R A S P G L L P V A N G N S L N K V I P A K S P P P P T H S T Q L G A P S R K P D L R V I T S Q A G K G L M H H L N N A Q R L G V S Q S T H S L T T P V V S V A T P S L L S Q G L P F S S M P T A Y N T D Y Q L T S A E L S S L P A F S S P G G L S L G N V T A W Q Q P Q Q P Q Q P Q Q P Q P P Q Q Q P P Q P Q Q P Q P Q Q P Q Q P Q Q P P Q Q Q S H L V P V S L S N L I P G S P L P H V G A A L T V T T H P H I S I K S E P V S P S R E R S P A P P P P A V F P A A R P E P G D G L S S P A G G S Y E T G D R D D G R G D F G P T L G L L R P A P E P E A E G S A V K R M R L D T W T L K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis (By similarity).
Alternative names
Myocyte-specific enhancer factor 2D, MEF2D
Recommended products
Recombinant Human MEF2D protein is a Human Full Length protein, in the 1 to 514 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q14814-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M G R K K I Q I Q R I T D E R N R R V T F T K R K F G L M K K A Y E L S V L C D C E I A L I I F N H S N K L F Q Y A S T D M D K V L L K Y T E Y N E P H E S R T N A D I I E T L R K K G F N G C D S P E P D G E D S L E Q S P L L E D K Y R R A S E E L D G L F R R Y G S T V P A P N F A M P V T V P V S N Q S S L Q F S N P S G S L V T P S L V T S S L T D P R L L S P Q Q P A L Q R N S V S P G L P Q R P A S A G A M L G G D L N S A N G A C P S P V G N G Y V S A R A S P G L L P V A N G N S L N K V I P A K S P P P P T H S T Q L G A P S R K P D L R V I T S Q A G K G L M H H L N N A Q R L G V S Q S T H S L T T P V V S V A T P S L L S Q G L P F S S M P T A Y N T D Y Q L T S A E L S S L P A F S S P G G L S L G N V T A W Q Q P Q Q P Q Q P Q Q P Q P P Q Q Q P P Q P Q Q P Q P Q Q P Q Q P Q Q P P Q Q Q S H L V P V S L S N L I P G S P L P H V G A A L T V T T H P H I S I K S E P V S P S R E R S P A P P P P A V F P A A R P E P G D G L S S P A G G S Y E T G D R D D G R G D F G P T L G L L R P A P E P E A E G S A V K R M R L D T W T L K
- Accession
- Q14814
- Protein length
- Full Length
- Predicted molecular weight
- 82.28 kDa
- Amino acids
- 1 to 514
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis (By similarity).
- Sequence similarities
Belongs to the MEF2 family.
- Post-translational modifications
Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phosphorylation on Ser-180 can be enhanced by EGF. Phosphorylated and activated by CaMK4.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEF2D also known as Myocyte Enhancer Factor 2D is a transcription factor protein with a mass of roughly 55 kDa. Scientists find MEF2D widely expressed in different tissues including muscle brain and immune cells where it regulates gene expression. By binding to specific DNA sequences MEF2D influences the transcription of various genes essential for cellular differentiation and development. MEF2D operates as an important player in controlling the expression of genes involved in muscle development and neuronal differentiation.
- Biological function summary
MEF2D plays a significant role in muscle and neuronal development and is part of the MEF2 family which includes other factors such as MEF2A MEF2B and MEF2C. These transcription factors form a complex with other proteins including histone deacetylases (HDACs) to regulate gene expression. MEF2D acts as a mediator between signaling pathways and genes responsible for cell growth survival and apoptosis. It helps modulate cellular responses to various external and internal cues adjusting transcriptional programs accordingly.
- Pathways
MEF2D is a significant component in the mitogen-activated protein kinase (MAPK) and calcium/calmodulin-dependent protein kinase (CaMK) signaling pathways. These pathways influence muscle differentiation and neuronal plasticity. MEF2D cooperates with related proteins such as CaMKIV which phosphorylates MEF2D leading to the activation of specific target genes. Its role interconnects with other members of the MEF2 family and influences cellular processes linked to differentiation and survival.
- Associated diseases and disorders
MEF2D has been implicated in conditions such as cancer and neurodegenerative diseases. In certain cancers altered regulation of MEF2D expression can lead to unchecked cellular proliferation. Its interaction with HDACs has also been observed in neurodegenerative disorders like Alzheimer's disease where dysregulated gene expression contributes to neuronal dysfunction. MEF2D may interact with other proteins like tau which associates with Alzheimer's pathology making it a target of interest in therapeutic strategies.
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1 product image
SDS-PAGE - Recombinant Human MEF2D protein (ab152520)
12.5% SDS-PAGE analysis of ab152520 stained with Coomassie Blue.
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