Recombinant Human Megf10 protein (His tag) is a Human Fragment protein, in the 1 to 857 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M V I S L N S C L S F I C L L L C H W I G T A S P L N L E D P N V C S H W E S Y S V T V Q E S Y P H P F D Q I Y Y T S C T D I L N W F K C T R H R V S Y R T A Y R H G E K T M Y R R K S Q C C P G F Y E S G E M C V P H C A D K C V H G R C I A P N T C Q C E P G W G G T N C S S A C D G D H W G P H C T S R C Q C K N G A L C N P I T G A C H C A A G F R G W R C E D R C E Q G T Y G N D C H Q R C Q C Q N G A T C D H V T G E C R C P P G Y T G A F C E D L C P P G K H G P Q C E Q R C P C Q N G G V C H H V T G E C S C P S G W M G T V C G Q P C P E G R F G K N C S Q E C Q C H N G G T C D A A T G Q C H C S P G Y T G E R C Q D E C P V G T Y G V L C A E T C Q C V N G G K C Y H V S G A C L C E A G F A G E R C E A R L C P E G L Y G I K C D K R C P C H L E N T H S C H P M S G E C A C K P G W S G L Y C N E T C S P G F Y G E A C Q Q I C S C Q N G A D C D S V T G K C T C A P G F K G I D C S T P C P L G T Y G I N C S S R C G C K N D A V C S P V D G S C T C K A G W H G V D C S I R C P S G T W G F G C N L T C Q C L N G G A C N T L D G T C T C A P G W R G E K C E L P C Q D G T Y G L N C A E R C D C S H A D G C H P T T G H C R C L P G W S G V H C D S V C A E G R W G P N C S L P C Y C K N G A S C S P D D G I C E C A P G F R G T T C Q R I C S P G F Y G H R C S Q T C P Q C V H S S G P C H H I T G L C D C L P G F T G A L C N E V C P S G R F G K N C A G I C T C T N N G T C N P I D R S C Q C Y P G W I G S D C S Q P C P P A H W G P N C I H T C N C H N G A F C S A Y D G E C K C T P G W T G L Y C T Q R C P L G F Y G K D C A L I C Q C Q N G A D C D H I S G Q C T C R T G F M G R H C E Q K C P S G T Y G Y G C R Q I C D C L N N S T C D H I T G T C Y C S P G W K G A R C D Q A G V I I V G N L N S L S R T S T A L P A D S Y Q I G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Membrane receptor involved in phagocytosis by macrophages and astrocytes of apoptotic cells. Receptor for C1q, an eat-me signal, that binds phosphatidylserine expressed on the surface of apoptotic cells (PubMed:27170117). Cooperates with ABCA1 within the process of engulfment. Promotes the formation of large intracellular vacuoles and may be responsible for the uptake of amyloid-beta peptides (PubMed:17643423, PubMed:20828568). Necessary for astrocyte-dependent apoptotic neuron clearance in the developing cerebellum (PubMed:27170117). Plays role in muscle cell proliferation, adhesion and motility. Is also an essential factor in the regulation of myogenesis. Controls the balance between skeletal muscle satellite cells proliferation and differentiation through regulation of the notch signaling pathway (PubMed:28498977, Ref.16). May also function in the mosaic spacing of specific neuron subtypes in the retina through homotypic retinal neuron repulsion. Mosaics provide a mechanism to distribute each cell type evenly across the retina, ensuring that all parts of the visual field have access to a full set of processing elements (PubMed:17498693, PubMed:17643423, PubMed:20828568, PubMed:22101682, PubMed:27170117, PubMed:28498977).
Alternative names
KIAA1780, MEGF10, Multiple epidermal growth factor-like domains protein 10, Multiple EGF-like domains protein 10
Recommended products
Recombinant Human Megf10 protein (His tag) is a Human Fragment protein, in the 1 to 857 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q96KG7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M V I S L N S C L S F I C L L L C H W I G T A S P L N L E D P N V C S H W E S Y S V T V Q E S Y P H P F D Q I Y Y T S C T D I L N W F K C T R H R V S Y R T A Y R H G E K T M Y R R K S Q C C P G F Y E S G E M C V P H C A D K C V H G R C I A P N T C Q C E P G W G G T N C S S A C D G D H W G P H C T S R C Q C K N G A L C N P I T G A C H C A A G F R G W R C E D R C E Q G T Y G N D C H Q R C Q C Q N G A T C D H V T G E C R C P P G Y T G A F C E D L C P P G K H G P Q C E Q R C P C Q N G G V C H H V T G E C S C P S G W M G T V C G Q P C P E G R F G K N C S Q E C Q C H N G G T C D A A T G Q C H C S P G Y T G E R C Q D E C P V G T Y G V L C A E T C Q C V N G G K C Y H V S G A C L C E A G F A G E R C E A R L C P E G L Y G I K C D K R C P C H L E N T H S C H P M S G E C A C K P G W S G L Y C N E T C S P G F Y G E A C Q Q I C S C Q N G A D C D S V T G K C T C A P G F K G I D C S T P C P L G T Y G I N C S S R C G C K N D A V C S P V D G S C T C K A G W H G V D C S I R C P S G T W G F G C N L T C Q C L N G G A C N T L D G T C T C A P G W R G E K C E L P C Q D G T Y G L N C A E R C D C S H A D G C H P T T G H C R C L P G W S G V H C D S V C A E G R W G P N C S L P C Y C K N G A S C S P D D G I C E C A P G F R G T T C Q R I C S P G F Y G H R C S Q T C P Q C V H S S G P C H H I T G L C D C L P G F T G A L C N E V C P S G R F G K N C A G I C T C T N N G T C N P I D R S C Q C Y P G W I G S D C S Q P C P P A H W G P N C I H T C N C H N G A F C S A Y D G E C K C T P G W T G L Y C T Q R C P L G F Y G K D C A L I C Q C Q N G A D C D H I S G Q C T C R T G F M G R H C E Q K C P S G T Y G Y G C R Q I C D C L N N S T C D H I T G T C Y C S P G W K G A R C D Q A G V I I V G N L N S L S R T S T A L P A D S Y Q I G
- Accession
- Q96KG7
- Protein length
- Fragment
- Predicted molecular weight
- 90.2 kDa
- Amino acids
- 1 to 857
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Membrane receptor involved in phagocytosis by macrophages and astrocytes of apoptotic cells. Receptor for C1q, an eat-me signal, that binds phosphatidylserine expressed on the surface of apoptotic cells (PubMed:27170117). Cooperates with ABCA1 within the process of engulfment. Promotes the formation of large intracellular vacuoles and may be responsible for the uptake of amyloid-beta peptides (PubMed:17643423, PubMed:20828568). Necessary for astrocyte-dependent apoptotic neuron clearance in the developing cerebellum (PubMed:27170117). Plays role in muscle cell proliferation, adhesion and motility. Is also an essential factor in the regulation of myogenesis. Controls the balance between skeletal muscle satellite cells proliferation and differentiation through regulation of the notch signaling pathway (PubMed:28498977, Ref.16). May also function in the mosaic spacing of specific neuron subtypes in the retina through homotypic retinal neuron repulsion. Mosaics provide a mechanism to distribute each cell type evenly across the retina, ensuring that all parts of the visual field have access to a full set of processing elements (PubMed:17498693, PubMed:17643423, PubMed:20828568, PubMed:22101682, PubMed:27170117, PubMed:28498977).
- Sequence similarities
Belongs to the MEGF family.
- Post-translational modifications
Phosphorylated on tyrosine residues. Phosphorylation at Tyr-1030 may be important for muscle cell proliferation.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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SDS-PAGE - Recombinant Human Megf10 protein (His tag) (ab276714)
SDS-PAGE analysis of ab276714
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