Recombinant Human MEK2 protein is a Human Full Length protein, in the 1 to 400 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M L A R R K P V L P A L T I N P T I A E G P S P T S E G A S E A N L V D L Q K K L E E L E L D E Q Q K K R L E A F L T Q K A K V G E L K D D D F E R I S E L G A G N G G V V T K V Q H R P S G L I M A R K L I H L E I K P A I R N Q I I R E L Q V L H E C N S P Y I V G F Y G A F Y S D G E I S I C M E H M D G G S L D Q V L K E A K R I P E E I L G K V S I A V L R G L A Y L R E K H Q I M H R D V K P S N I L V N S R G E I K L C D F G V S G Q L I D S M A N S F V G T R S Y M A P E R L Q G T H Y S V Q S D I W S M G L S L V E L A V G R Y P I P P P D A K E L E A I F G R P V V D G E E G E P H S I S P R P R P P G R P V S G H G M D S R P A M A I F E L L D Y I V N E P P P K L P N G V F T P D F Q E F V N K C L I K N P A E R A D L K M L T N H T F I K R S E V E E V D F A G W L C K T L R L N Q P G T P T R T A V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126).
Alternative names
MEK2, MKK2, PRKMK2, MAP2K2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2
Recommended products
Recombinant Human MEK2 protein is a Human Full Length protein, in the 1 to 400 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P36507-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M L A R R K P V L P A L T I N P T I A E G P S P T S E G A S E A N L V D L Q K K L E E L E L D E Q Q K K R L E A F L T Q K A K V G E L K D D D F E R I S E L G A G N G G V V T K V Q H R P S G L I M A R K L I H L E I K P A I R N Q I I R E L Q V L H E C N S P Y I V G F Y G A F Y S D G E I S I C M E H M D G G S L D Q V L K E A K R I P E E I L G K V S I A V L R G L A Y L R E K H Q I M H R D V K P S N I L V N S R G E I K L C D F G V S G Q L I D S M A N S F V G T R S Y M A P E R L Q G T H Y S V Q S D I W S M G L S L V E L A V G R Y P I P P P D A K E L E A I F G R P V V D G E E G E P H S I S P R P R P P G R P V S G H G M D S R P A M A I F E L L D Y I V N E P P P K L P N G V F T P D F Q E F V N K C L I K N P A E R A D L K M L T N H T F I K R S E V E E V D F A G W L C K T L R L N Q P G T P T R T A V
- Accession
- P36507
- Protein length
- Full Length
- Predicted molecular weight
- 46.9 kDa
- Amino acids
- 1 to 400
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab124543 was purified using conventional chromatography techniques.
General info
- Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126).
- Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Post-translational modifications
MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEK2 or MAP2K2 is a protein kinase with a mass of approximately 44 kDa. This kinase part of the Mitogen-Activated Protein Kinase (MAPK) pathway primarily functions mechanically as a dual-specificity protein kinase. It phosphorylates both serine/threonine and tyrosine residues on its substrate proteins. MEK2 is largely expressed in various tissues including the brain and heart. It serves an important role in relaying extracellular signals within cells leading to diverse cellular responses.
- Biological function summary
MEK2 participates in signal transduction pathways mediating cell division differentiation and apoptosis. As a part of the MAPK signaling cascade it operates downstream of RAS and RAF proteins. MEK2 forms a complex with MEK1 another closely related kinase to ensure proper signal relay. This complex plays a significant role in the regulation of cellular responses to growth signals and stress.
- Pathways
MEK2 is intricately connected to the ERK1/2 pathway one of the key pathways it is involved in. ERK1/2 (Extracellular signal-Regulated Kinases) further transmits signals to the cell's nuclear components regulating gene expression critically. MEK2 directly phosphorylates and activates ERK1 and ERK2 facilitating their role in transcription cell cycle regulation and maintenance of cell integrity. The interaction of MEK2 with proteins like BRAF and CRAF along the MAPK pathway amplifies the signal transduction for various growth factors and hormones.
- Associated diseases and disorders
MEK2 has a notable association with cancer particularly melanoma and colorectal cancer. It often becomes aberrantly activated leading to unregulated cell proliferation. The connection of MEK2 to ERK1/2 proteins in these malignancies makes it a potential therapeutic target. Furthermore MEK2 mutations can contribute to Noonan syndrome a disorder that affects multiple body systems marking its relationship with genetic abnormalities and developmental issues. Researchers have been investigating MEK inhibitors to target these disorders highlighting the importance of understanding MEK2's role in disease contexts.
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1 product image
SDS-PAGE - Recombinant Human MEK2 protein (ab124543)
15% SDS-PAGE analysis of 3 μg ab124543.
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