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AB105578

Recombinant Human MEK3 protein (His tag N-Terminus)

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(1 Publication)

Recombinant Human MEK3 protein (His tag N-Terminus) is a Human Fragment protein, in the 20 to 347 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

MEK3, MKK3, PRKMK3, SKK2, MAP2K3, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, MEK 3, SAPK kinase 2, SAPKK-2, SAPKK2

1 Images
SDS-PAGE - Recombinant Human MEK3 protein (His tag N-Terminus) (AB105578)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MEK3 protein (His tag N-Terminus) (AB105578)

15% SDS-PAGE analysis of ab105578 (3ug)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P46734

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSKPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMAVKRIRATVNSQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFYRKVLDKNMTIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS","proteinLength":"Fragment","predictedMolecularWeight":"38.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":347,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P46734","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK3 also known as MAP2K3 is a kinase that plays a significant mechanical role within cells. It is part of the MAP kinase family sharing functions with enzymes like MEK6. MEK3 has a molecular mass of approximately 38 kDa and is localized in cytoplasmic regions of various cell types. This protein is expressed in a wide range of tissues indicating its diverse functional roles across different biological systems.
Biological function summary

MEK3 is involved in the phosphorylation cascade within the mitogen-activated protein kinase (MAPK) pathway. It often exists as part of a complex working alongside other kinases to activate p38 MAPK which then regulates gene expression. The involvement of MEK3 in this signaling cascade influences processes such as cellular stress response and inflammation. Its ability to modulate these processes makes MEK3 an important component in maintaining cellular homeostasis.

Pathways

MEK3 functions as an essential link within the stress-activated pathways like the MAPK signaling pathway. It phosphorylates and activates the p38 MAPK pathway therefore regulating activities related to cellular stress and inflammatory responses. MEK3 works in conjunction with related kinases such as MEK6 to propagate the MAPK signaling cascade further emphasizing its important role in these pathways.

MEK3 has a documented involvement in inflammatory conditions and cancer. Its role in activating the p38 MAPK pathway connects it to inflammatory diseases where dysregulated signaling may lead to excessive inflammation. Furthermore aberrant MEK3 activity is associated with certain cancers as it affects cell proliferation and survival. MEK3's interactions with other kinases like MEK6 underline its involvement in these pathological processes making it a potential target for therapeutic intervention.
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Specifications

Form

Liquid

Additional notes

ab105578 was purified using conventional chromatography.

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General info

Function

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Post-translational modifications

Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases positively regulates the kinase activity (PubMed:8622669). Phosphorylated by TAOK2 (PubMed:11279118).. (Microbial infection) Yersinia YopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

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Product protocols

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Target data

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
See full target information MAP2K3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 11:305 PubMed32366830

2020

Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Hsuan Wu,Ting-Fang Chou,Leslie Young,Fu-Yi Hsieh,Hsuan-Yin Pan,Shu-Ting Mo,Shani Bialik Brown,Ruey-Hwa Chen,Adi Kimchi,Ming-Zong Lai
View all publications
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