Recombinant Human MEK3 protein is a Human Fragment protein, in the 20 to 347 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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- Cell death & disease 11:3052020Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYung-Hsuan Wu et. al.PubMed 32366830
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S K P P A P N P T P P R N L D S R T F I T I G D R N F E V E A D D L V T I S E L G R G A Y G V V E K V R H A Q S G T I M A V K R I R A T V N S Q E Q K R L L M D L D I N M R T V D C F Y T V T F Y G A L F R E G D V W I C M E L M D T S L D K F Y R K V L D K N M T I P E D I L G E I A V S I V R A L E H L H S K L S V I H R D V K P S N V L I N K E G H V K M C D F G I S G Y L V D S V A K T M D A G C K P Y M A P E R I N P E L N Q K G Y N V K S D V W S L G I T M I E M A I L R F P Y E S W G T P F Q Q L K Q V V E E P S P Q L P A D R F S P E F V D F T A Q C L R K N P A E R M S Y L E L M E H P F F T L H K T K K T D I A A F V K E I L G E D S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
Alternative names
MEK3, MKK3, PRKMK3, SKK2, MAP2K3, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, MEK 3, SAPK kinase 2, SAPKK-2, SAPKK2
Recommended products
Recombinant Human MEK3 protein is a Human Fragment protein, in the 20 to 347 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P46734-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S K P P A P N P T P P R N L D S R T F I T I G D R N F E V E A D D L V T I S E L G R G A Y G V V E K V R H A Q S G T I M A V K R I R A T V N S Q E Q K R L L M D L D I N M R T V D C F Y T V T F Y G A L F R E G D V W I C M E L M D T S L D K F Y R K V L D K N M T I P E D I L G E I A V S I V R A L E H L H S K L S V I H R D V K P S N V L I N K E G H V K M C D F G I S G Y L V D S V A K T M D A G C K P Y M A P E R I N P E L N Q K G Y N V K S D V W S L G I T M I E M A I L R F P Y E S W G T P F Q Q L K Q V V E E P S P Q L P A D R F S P E F V D F T A Q C L R K N P A E R M S Y L E L M E H P F F T L H K T K K T D I A A F V K E I L G E D S
- Accession
- P46734
- Protein length
- Fragment
- Predicted molecular weight
- 38.3 kDa
- Amino acids
- 20 to 347
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab105578 was purified using conventional chromatography.
General info
- Function
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
- Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Post-translational modifications
Autophosphorylated. Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases positively regulates the kinase activity (PubMed:8622669). Phosphorylated by TAOK2 (PubMed:11279118).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEK3 also known as MAP2K3 is a kinase that plays a significant mechanical role within cells. It is part of the MAP kinase family sharing functions with enzymes like MEK6. MEK3 has a molecular mass of approximately 38 kDa and is localized in cytoplasmic regions of various cell types. This protein is expressed in a wide range of tissues indicating its diverse functional roles across different biological systems.
- Biological function summary
MEK3 is involved in the phosphorylation cascade within the mitogen-activated protein kinase (MAPK) pathway. It often exists as part of a complex working alongside other kinases to activate p38 MAPK which then regulates gene expression. The involvement of MEK3 in this signaling cascade influences processes such as cellular stress response and inflammation. Its ability to modulate these processes makes MEK3 an important component in maintaining cellular homeostasis.
- Pathways
MEK3 functions as an essential link within the stress-activated pathways like the MAPK signaling pathway. It phosphorylates and activates the p38 MAPK pathway therefore regulating activities related to cellular stress and inflammatory responses. MEK3 works in conjunction with related kinases such as MEK6 to propagate the MAPK signaling cascade further emphasizing its important role in these pathways.
- Associated diseases and disorders
MEK3 has a documented involvement in inflammatory conditions and cancer. Its role in activating the p38 MAPK pathway connects it to inflammatory diseases where dysregulated signaling may lead to excessive inflammation. Furthermore aberrant MEK3 activity is associated with certain cancers as it affects cell proliferation and survival. MEK3's interactions with other kinases like MEK6 underline its involvement in these pathological processes making it a potential target for therapeutic intervention.
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SDS-PAGE - Recombinant Human MEK3 protein (ab105578)
15% SDS-PAGE analysis of ab105578 (3ug)
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