Recombinant Human Melanoma Inhibitory Activity (MIA) Protein (His-tag) is a Human Full Length protein, expressed in HEK 293, with >=95% purity and suitable for HPLC, MS, SDS-PAGE.
Images
Key facts
- Purity
- >=95% HPLC
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
G P M P K L A D R K L C A D Q E C S H P I S M A V A L Q D Y M A P D C R F L T I H R G Q V V Y V F S K L K G R G R L F W G G S V Q G D Y Y G D L A A R L G Y F P S S I V R E D Q T L K P G K V D V K T D K W D F Y C Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
Alternative names
Melanoma-derived growth regulatory protein, Melanoma inhibitory activity protein, MIA
Recommended products
Recombinant Human Melanoma Inhibitory Activity (MIA) Protein (His-tag) is a Human Full Length protein, expressed in HEK 293, with >=95% purity and suitable for HPLC, MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Expression system
- HEK 293 cells
- Tags
- His tag N-Terminus
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- Q16674-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- G P M P K L A D R K L C A D Q E C S H P I S M A V A L Q D Y M A P D C R F L T I H R G Q V V Y V F S K L K G R G R L F W G G S V Q G D Y Y G D L A A R L G Y F P S S I V R E D Q T L K P G K V D V K T D K W D F Y C Q
- Accession
- Q16674
- Protein length
- Full Length
- Predicted molecular weight
- 14.23 kDa
- Actual molecular weight
- 14.23 kDa
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
- Sequence similarities
Belongs to the MIA/OTOR family.
- Post-translational modifications
May possess two intramolecular disulfide bonds.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
SDS-PAGE - Recombinant Human Melanoma Inhibitory Activity (MIA) Protein (His-tag) (ab317881)
SDS-PAGE analysis of ab317881 under reducing conditions for 2ug protein.
Mass Spectrometry - Recombinant Human Melanoma Inhibitory Activity (MIA) Protein (His-tag) (ab317881)
Mass determination by ESI-TOF. Predicted MW is 14230 Da (+/- 10 Da by ESI-TOF). Observed MW is 14231.08 Da.
HPLC - Recombinant Human Melanoma Inhibitory Activity (MIA) Protein (His-tag) (ab317881)
HPLC analysis of ab317881
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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