Recombinant human MELK protein (Active) is a Human Fragment protein, in the 1 to 550 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M K D Y D E L L K Y Y E L H E T I G T G G F A K V K L A C H I L T G E M V A I K I M D K N T L G S D L P R I K T E I E A L K N L R H Q H I C Q L Y H V L E T A N K I F M V L E Y C P G G E L F D Y I I S Q D R L S E E E T R V V F R Q I V S A V A Y V H S Q G Y A H R D L K P E N L L F D E Y H K L K L I D F G L C A K P K G N K D Y H L Q T C C G S L A Y A A P E L I Q G K S Y L G S E A D V W S M G I L L Y V L M C G F L P F D D D N V M A L Y K K I M R G K Y D V P K W L S P S S I L L L Q Q M L Q V D P K K R I S M K N L L N H P W I M Q D Y N Y P V E W Q S K N P F I H L D D D C V T E L S V H H R N N R Q T M E D L I S L W Q Y D H L T A T Y L L L L A K K A R G K P V R L R L S S F S C G Q A S A T P F T D I K S N N W S L E D V T A S D K N Y V A G L I D Y D W C E D D L S T G A A T P R T S Q F T K Y W T E S N G V E S K S L T P A L C R T P A N K L K N K E N V Y T P K S A V K N E E Y F M F P E P K T P V N K N Q H K R E I L T T P N R Y T T P S K A R N Q C L K E T P I K I P V N S T G T D K L M T G V I S P E R R C R S V E L D L N Q A H M E E T P K R K G A K V F G S L E R G L D K V I T V L T R S K R K G S A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
Alternative names
KIAA0175, MELK, Maternal embryonic leucine zipper kinase, hMELK, Protein kinase Eg3, Protein kinase PK38, Tyrosine-protein kinase MELK, pEg3 kinase, hPK38
Recommended products
Recombinant human MELK protein (Active) is a Human Fragment protein, in the 1 to 550 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab204155 was determined to be 210 nmol/min/mg.
- Accession
- Q14680-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M K D Y D E L L K Y Y E L H E T I G T G G F A K V K L A C H I L T G E M V A I K I M D K N T L G S D L P R I K T E I E A L K N L R H Q H I C Q L Y H V L E T A N K I F M V L E Y C P G G E L F D Y I I S Q D R L S E E E T R V V F R Q I V S A V A Y V H S Q G Y A H R D L K P E N L L F D E Y H K L K L I D F G L C A K P K G N K D Y H L Q T C C G S L A Y A A P E L I Q G K S Y L G S E A D V W S M G I L L Y V L M C G F L P F D D D N V M A L Y K K I M R G K Y D V P K W L S P S S I L L L Q Q M L Q V D P K K R I S M K N L L N H P W I M Q D Y N Y P V E W Q S K N P F I H L D D D C V T E L S V H H R N N R Q T M E D L I S L W Q Y D H L T A T Y L L L L A K K A R G K P V R L R L S S F S C G Q A S A T P F T D I K S N N W S L E D V T A S D K N Y V A G L I D Y D W C E D D L S T G A A T P R T S Q F T K Y W T E S N G V E S K S L T P A L C R T P A N K L K N K E N V Y T P K S A V K N E E Y F M F P E P K T P V N K N Q H K R E I L T T P N R Y T T P S K A R N Q C L K E T P I K I P V N S T G T D K L M T G V I S P E R R C R S V E L D L N Q A H M E E T P K R K G A K V F G S L E R G L D K V I T V L T R S K R K G S A
- Accession
- Q14680
- Protein length
- Fragment
- Predicted molecular weight
- 88 kDa
- Amino acids
- 1 to 550
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.
- Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
- Post-translational modifications
Autophosphorylated: autophosphorylation of the T-loop at Thr-167 and Ser-171 is required for activation. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8 (Probable).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Maternal embryonic leucine zipper kinase commonly referred to as MELK is a serine/threonine protein kinase with a molecular mass of about 74 kDa. MELK is broadly expressed in both human and animal tissues with significant presence in embryonic and adult stem cells. Researchers often recognize it as MELK or MELK-0A21 in studies. The protein is characterized by its regulation of cell division and apoptosis making it an important player in cellular proliferation.
- Biological function summary
MELK regulates signals that control the cell cycle apoptosis and embryonic development. MELK works as a component of complexes involved in maintaining cellular homeostasis. The protruding roles involve influencing cellular architecture and governance of stem cell maintenance. Studies in animal models show MELK involvement in maintaining pluripotency and regulation during the development phase.
- Pathways
MELK participates in key processes such as the cell cycle and apoptosis pathways. Its diverse functionality connects MELK to proteins like Bcl-2-associated death promoter (BAD) and p53 which further influence main cellular events. These pathways and interactions make MELK a target of interest in the study of cellular growth and programmed cell death.
- Associated diseases and disorders
Researchers link MELK to cancer and neurodegenerative diseases. The overexpression of MELK is frequently observed in various cancer types making it relevant to oncogenesis. Studies examine its relation to proteins like cyclin-dependent kinases (CDKs) and survivin in cancer progression. Additionally MELK-0A21 variants may offer insights in disorders like Glioblastoma where abnormal MELK activity aligns with disease aggression.
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4 product images
Functional Studies - Recombinant human MELK protein (Active) (ab204155)
The specific activity of MELK (ab204155) was determined to be 140 nmol/min/mg as per activity assay protocol and was equivalent to 175 nmol/min/mg as per radiometric assay
SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)
SDS PAGE analysis of ab204155
Functional Studies - Recombinant human MELK protein (Active) (ab204155)
The specific activity of ab204155 was determined to be 210 nmol/min/mg.
SDS-PAGE - Recombinant human MELK protein (Active) (ab204155)
SDS-PAGE analysis of ab204155 which was determined to be >85% pure by densitometry.
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