Recombinant Human Meprin beta protein (His tag) is a Human Fragment protein, in the 1 to 652 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M D L W N L S W F L F L D A L L V I S G L A T P E N F D V D G G M D Q D I F D I N E G L G L D L F E G D I R L D R A Q I R N S I I G E K Y R W P H T I P Y V L E D S L E M N A K G V I L N A F E R Y R L K T C I D F K P W A G E T N Y I S V F K G S G C W S S V G N R R V G K Q E L S I G A N C D R I A T V Q H E F L H A L G F W H E Q S R S D R D D Y V R I M W D R I L S G R E H N F N T Y S D D I S D S L N V P Y D Y T S V M H Y S K T A F Q N G T E P T I V T R I S D F E D V I G Q R M D F S D S D L L K L N Q L Y N C S S S L S F M D S C S F E L E N V C G M I Q S S G D N A D W Q R V S Q V P R G P E S D H S N M G Q C Q G S G F F M H F D S S S V N V G A T A V L E S R T L Y P K R G F Q C L Q F Y L Y N S G S E S D Q L N I Y I R E Y S A D N V D G N L T L V E E I K E I P T G S W Q L Y H V T L K V T K K F R V V F E G R K G S G A S L G G L S I D D I N L S E T R C P H H I W H I R N F T Q F I G S P N G T L Y S P P F Y S S K G Y A F Q I Y L N L A H V T N A G I Y F H L I S G A N D D Q L Q W P C P W Q Q A T M T L L D Q N P D I R Q R M S N Q R S I T T D P F M T T D N G N Y F W D R P S K V G T V A L F S N G T Q F R R G G G Y G T S A F I T H E R L K S R D F I K G D D V Y I L L T V E D I S H L N S T Q I Q L T P A P S V Q D L C S K T T C K N D G V C T V R D G K A E C R C Q S G E D W W Y M G E R C E K R G S T R D T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components. Cleaves also the amyloid precursor protein/APP, thereby releasing neurotoxic amyloid beta peptides (PubMed:27180357).
Alternative names
Meprin A subunit beta, Endopeptidase-2, Meprin B, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit beta, PABA peptide hydrolase, PPH beta, MEP1B
Recommended products
Recombinant Human Meprin beta protein (His tag) is a Human Fragment protein, in the 1 to 652 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q16820-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M D L W N L S W F L F L D A L L V I S G L A T P E N F D V D G G M D Q D I F D I N E G L G L D L F E G D I R L D R A Q I R N S I I G E K Y R W P H T I P Y V L E D S L E M N A K G V I L N A F E R Y R L K T C I D F K P W A G E T N Y I S V F K G S G C W S S V G N R R V G K Q E L S I G A N C D R I A T V Q H E F L H A L G F W H E Q S R S D R D D Y V R I M W D R I L S G R E H N F N T Y S D D I S D S L N V P Y D Y T S V M H Y S K T A F Q N G T E P T I V T R I S D F E D V I G Q R M D F S D S D L L K L N Q L Y N C S S S L S F M D S C S F E L E N V C G M I Q S S G D N A D W Q R V S Q V P R G P E S D H S N M G Q C Q G S G F F M H F D S S S V N V G A T A V L E S R T L Y P K R G F Q C L Q F Y L Y N S G S E S D Q L N I Y I R E Y S A D N V D G N L T L V E E I K E I P T G S W Q L Y H V T L K V T K K F R V V F E G R K G S G A S L G G L S I D D I N L S E T R C P H H I W H I R N F T Q F I G S P N G T L Y S P P F Y S S K G Y A F Q I Y L N L A H V T N A G I Y F H L I S G A N D D Q L Q W P C P W Q Q A T M T L L D Q N P D I R Q R M S N Q R S I T T D P F M T T D N G N Y F W D R P S K V G T V A L F S N G T Q F R R G G G Y G T S A F I T H E R L K S R D F I K G D D V Y I L L T V E D I S H L N S T Q I Q L T P A P S V Q D L C S K T T C K N D G V C T V R D G K A E C R C Q S G E D W W Y M G E R C E K R G S T R D T
- Accession
- Q16820
- Protein length
- Fragment
- Predicted molecular weight
- 73 kDa
- Amino acids
- 1 to 652
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Membrane metallopeptidase that sheds many membrane-bound proteins. Exhibits a strong preference for acidic amino acids at the P1' position. Known substrates include: FGF19, VGFA, IL1B, IL18, procollagen I and III, E-cadherin, KLK7, gastrin, ADAM10, tenascin-C. The presence of several pro-inflammatory cytokine among substrates implicate MEP1B in inflammation. It is also involved in tissue remodeling due to its capability to degrade extracellular matrix components. Cleaves also the amyloid precursor protein/APP, thereby releasing neurotoxic amyloid beta peptides (PubMed:27180357).
- Post-translational modifications
Phosphorylated by PKC at multiple sites of its cytoplasmic part. Phosphorylation dcreases activity at the cell surface, leading to diminished substrate cleavage.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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SDS-PAGE - Recombinant Human Meprin beta protein (His tag) (ab276308)
SDS-PAGE analysis of ab276308
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