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Recombinant Human Mesothelin protein is a Human Fragment protein, in the 37 to 286 aa range, expressed in CHO, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.

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Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
CHO cells
Tags
Tag free
Applications
SDS-PAGE, FuncS, HPLC
Biologically active
No

Amino acid sequence

L A G E T G Q E A A P L D G V L A N P P N I S S L S P R Q L L G F P C A E V S G L S T E R V R E L A V A L A Q K N V K L S T E Q L R C L A H R L S E P P E D L D A L P L D L L L F L N P D A F S G P Q A C T R F F S R I T K A N V D L L P R G A P E R Q R L L P A A L A C W G V R G S L L S E A D V R A L G G L A C D L P G R F V A E S A E V L L P R L V S C P G P L D Q D Q Q E A A R A A L Q G G G P P Y G P P S T W S V S T M D A L R G L L P V L G Q P I I R S I P Q G I V A A W R Q R S S R D P S W R Q P E R

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

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Target data

Function

Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

Alternative names

Recommended products

Recombinant Human Mesothelin protein is a Human Fragment protein, in the 37 to 286 aa range, expressed in CHO, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.

Key facts

Purity
>95% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
CHO cells
Applications
SDS-PAGE, FuncS, HPLC
Accession
Q13421-1
Animal free
No
Species
Human
Reconstitution
Reconstitute in water to 0.1 - 1.0 mg/ml.
Concentration
Loading...
Storage buffer

Constituents: 0.16% Sodium phosphate

Sequence info

Amino acid sequence

L A G E T G Q E A A P L D G V L A N P P N I S S L S P R Q L L G F P C A E V S G L S T E R V R E L A V A L A Q K N V K L S T E Q L R C L A H R L S E P P E D L D A L P L D L L L F L N P D A F S G P Q A C T R F F S R I T K A N V D L L P R G A P E R Q R L L P A A L A C W G V R G S L L S E A D V R A L G G L A C D L P G R F V A E S A E V L L P R L V S C P G P L D Q D Q Q E A A R A A L Q G G G P P Y G P P S T W S V S T M D A L R G L L P V L G Q P I I R S I P Q G I V A A W R Q R S S R D P S W R Q P E R
Accession
Q13421
Protein length
Fragment
Predicted molecular weight
26.7 kDa
Amino acids
37 to 286
Nature
Recombinant

Specifications

Form
Lyophilized
Additional notes

Greater than 95% by HPLC analyses.

General info

Function

Membrane-anchored forms may play a role in cellular adhesion.

Sequence similarities

Belongs to the mesothelin family.

Post-translational modifications

Both MPF and the cleaved form of mesothelin are N-glycosylated.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

As a result of glycosylation, ab245958 migrates with an apparent molecular mass of approximately 32-33 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.

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