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AB116871

Recombinant Human MEST protein (GST tag N-Terminus)

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Recombinant Human MEST protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 335 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

PEG1, MEST, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein

1 Images
SDS-PAGE - Recombinant Human MEST protein (GST tag N-Terminus) (AB116871)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MEST protein (GST tag N-Terminus) (AB116871)

12.5% SDS-PAGE gel showing ab116871, at approximately 62.92kDa, stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

Q5EB52

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB151564

Anti-MEST antibody [EPR6970(2)]

RabMAb|Recombinant

Western blot - Anti-MEST antibody [EPR6970(2)] (AB151564)

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  • 0
Primary Antibodies

AB171978

Anti-TIMMDC1/C3orf1 antibody [EPR11807]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TIMMDC1/C3orf1 antibody [EPR11807] (AB171978)

  • 0
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Proteins & Peptides

AB132633

Recombinant Human EPS8L2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human EPS8L2 protein (GST tag N-Terminus) (AB132633)

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  • 0
Proteins & Peptides

AB276334

Recombinant Human ECE1 protein (His tag)

SDS-PAGE - Recombinant Human ECE1 protein (His tag) (AB276334)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein.</p>" } } }
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Sequence info

[{"sequence":"MVRRDRLRRMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWKSSGKFFTYKGLRIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPILTRLMNFFVFSRGLTPVFGPYTRPSESELWDMWAGIRNNDGNLVIDSLLQYINQRKKFRRRWVGALASVTIPIHFIYGPLDPVNPYPEFLELYRKTLPRSTVSILDDHISHYPQLEDPMGFLNAYMGFINSF","proteinLength":"Full Length","predictedMolecularWeight":"62.92 kDa","actualMolecularWeight":null,"aminoAcidEnd":335,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q5EB52","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'MEST' protein also often referred to as the 'MEST band' is an imprinted gene product with molecular mass approximately 40 kDa. It displays biallelic and tissue-specific expression being especially present in tissues like placenta fetal tissues and adipose tissue. MEST also plays a critical role in development and metabolism providing an essential regulatory function in these tissues. The protein's expression levels can vary due to genomic imprinting which leads to selective expression from the paternal allele in certain contexts.
Biological function summary

The MEST protein participates in key cellular processes such as embryonic growth and adipocyte metabolism. It has a role in fetal development influencing growth rates and differentiation in tissues where it is expressed. If included in complex formations MEST may interact with other proteins to modulate signals that are important in these developmental processes. Given its involvement in adipogenesis MEST is also significant in energy homeostasis and fat storage impacting the body's metabolic responses.

Pathways

The MEST protein integrates into the adipogenesis signaling pathway contributing to the formation and function of adipose tissue. Additionally MEST is implicated in the insulin signaling pathway linking it to glucose metabolism and insulin sensitivity. Through these pathways MEST engages with other proteins such as PPARγ (Peroxisome proliferator-activated receptor gamma) and CEBPα (CCAAT/enhancer-binding protein alpha) which further highlights its involvement in regulating adipocyte differentiation and metabolic functions.

Alterations in MEST expression levels associate with various metabolic syndromes such as obesity and type 2 diabetes. These conditions reveal how improper regulation of MEST can disrupt energy balance and glucose metabolism. Its link to PPARγ highlights a connection to lipid disorders as this protein is important for lipid metabolism and any dysregulation might contribute to the development of metabolic-related diseases.
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Specifications

Form

Liquid

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General info

Function

Plays an important role in enabling neurons to transition from bipolar to multipolar shapes, a process essential for their correct migration toward the cortical plate during brain development. This function is likely achieved through the regulation of N-cadherin-dependent cell adhesion and the modulation of Akt and canonical Wnt signaling pathways. Essential for the development and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. Negatively regulates the canonical Wnt signaling pathway during adipogenic differentiation by reducing the glycosylation, maturation, and cell membrane localization of the Wnt coreceptor LRP6.

Sequence similarities

Belongs to the AB hydrolase superfamily.

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Product protocols

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Target data

Plays an important role in enabling neurons to transition from bipolar to multipolar shapes, a process essential for their correct migration toward the cortical plate during brain development. This function is likely achieved through the regulation of N-cadherin-dependent cell adhesion and the modulation of Akt and canonical Wnt signaling pathways. Essential for the development and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. Negatively regulates the canonical Wnt signaling pathway during adipogenic differentiation by reducing the glycosylation, maturation, and cell membrane localization of the Wnt coreceptor LRP6.
See full target information MEST
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Product promise

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