JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB116871

Recombinant Human MEST protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human MEST protein is a Human Full Length protein, in the 1 to 335 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

PEG1, MEST, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein

1 Images
SDS-PAGE - Recombinant Human MEST protein (AB116871)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MEST protein (AB116871)

12.5% SDS-PAGE gel showing ab116871, at approximately 62.92kDa, stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

Q5EB52

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein.</p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MVRRDRLRRMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWKSSGKFFTYKGLRIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPILTRLMNFFVFSRGLTPVFGPYTRPSESELWDMWAGIRNNDGNLVIDSLLQYINQRKKFRRRWVGALASVTIPIHFIYGPLDPVNPYPEFLELYRKTLPRSTVSILDDHISHYPQLEDPMGFLNAYMGFINSF","proteinLength":"Full Length","predictedMolecularWeight":"62.92 kDa","actualMolecularWeight":null,"aminoAcidEnd":335,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q5EB52","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 'MEST' protein also often referred to as the 'MEST band' is an imprinted gene product with molecular mass approximately 40 kDa. It displays biallelic and tissue-specific expression being especially present in tissues like placenta fetal tissues and adipose tissue. MEST also plays a critical role in development and metabolism providing an essential regulatory function in these tissues. The protein's expression levels can vary due to genomic imprinting which leads to selective expression from the paternal allele in certain contexts.
Biological function summary

The MEST protein participates in key cellular processes such as embryonic growth and adipocyte metabolism. It has a role in fetal development influencing growth rates and differentiation in tissues where it is expressed. If included in complex formations MEST may interact with other proteins to modulate signals that are important in these developmental processes. Given its involvement in adipogenesis MEST is also significant in energy homeostasis and fat storage impacting the body's metabolic responses.

Pathways

The MEST protein integrates into the adipogenesis signaling pathway contributing to the formation and function of adipose tissue. Additionally MEST is implicated in the insulin signaling pathway linking it to glucose metabolism and insulin sensitivity. Through these pathways MEST engages with other proteins such as PPARγ (Peroxisome proliferator-activated receptor gamma) and CEBPα (CCAAT/enhancer-binding protein alpha) which further highlights its involvement in regulating adipocyte differentiation and metabolic functions.

Alterations in MEST expression levels associate with various metabolic syndromes such as obesity and type 2 diabetes. These conditions reveal how improper regulation of MEST can disrupt energy balance and glucose metabolism. Its link to PPARγ highlights a connection to lipid disorders as this protein is important for lipid metabolism and any dysregulation might contribute to the development of metabolic-related diseases.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Sequence similarities

Belongs to the AB hydrolase superfamily.

style
left-column

Product protocols

style
left-column

Target data

See full target information MEST
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB151564

Anti-MEST antibody [EPR6970(2)]

RabMAb|Recombinant|Trial Size

Western blot - Anti-MEST antibody [EPR6970(2)] (AB151564)

  • 0
  • 0
Primary Antibodies

AB171978

Anti-TIMMDC1/C3orf1 antibody [EPR11807]

RabMAb|Recombinant

Western blot - Anti-TIMMDC1/C3orf1 antibody [EPR11807] (AB171978)

  • 0
  • 0
Proteins & Peptides

AB132633

Recombinant Human EPS8L2 protein

SDS-PAGE - Recombinant Human EPS8L2 protein (AB132633)

  • 0
  • 0
Proteins & Peptides

AB276334

Recombinant Human ECE1 protein (His tag)

SDS-PAGE - Recombinant Human ECE1 protein (His tag) (AB276334)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com