Recombinant Human Metnase protein (DDDDK tag N-Terminus)

Specifications

Form

Liquid

General info

Function

Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Also has kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity (PubMed : 16332963, PubMed : 16672366, PubMed : 17403897, PubMed : 17877369, PubMed : 18263876, PubMed : 20521842, PubMed : 22231448, PubMed : 24573677). In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining (PubMed : 16332963, PubMed : 21187428, PubMed : 22231448). Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A (PubMed : 18790802, PubMed : 20457750).

Sequence similarities

In the N-terminal section; belongs to the class V-like SAM-binding methyltransferase superfamily.. In the C-terminal section; belongs to the mariner transposase family.

Post-translational modifications

Methylated. Methylation regulates activity in DNA decatenation.. Phosphorylated at Ser-508 by CHEK1 and dephosphorylated by protein phosphatase 2A/PP2A. Phosphorylation at Ser-508 is enhanced by DNA damage and promotes recruitment to damaged DNA. It stimulates DNA repair and impairs replication fork restart.

Subcellular localisation

Nucleus