Recombinant Human Metnase protein is a Human Fragment protein, in the 27 to 684 aa range, expressed in Mammalian, with >=30% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=30% SDS-PAGE
- Expression system
- Mammalian
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- No
Amino acid sequence
Q L D V A C G Q E N L P V G A W P P G A A P A P F Q Y T P D H V V G P G A D I D P T Q I T F P G C I C V K T P C L P G T C S C L R H G E N Y D D N S C L R D I G S G G K Y A E P V F E C N V L C R C S D H C R N R V V Q K G L Q F H F Q V F K T H K K G W G L R T L E F I P K G R F V C E Y A G E V L G F S E V Q R R I H L Q T K S D S N Y I I A I R E H V Y N G Q V M E T F V D P T Y I G N I G R F L N H S C E P N L L M I P V R I D S M V P K L A L F A A K D I V P E E E L S Y D Y S G R Y L N L T V S E D K E R L D H G K L R K P C Y C G A K S C T A F L P F D S S L Y C P V E K S N I S C G N E K E P S M C G S A P S V F P S C K R L T L E T M K M M L D K K Q I R A I F L F E F K M G R K A A E T T R N I N N A F G P G T A N E R T V Q W W F K K F C K G D E S L E D E E R S G R P S E V D N D Q L R A I I E A D P L T T T R E V A E E L N V N H S T V V R H L K Q I G K V K K L D K W V P H E L T E N Q K N R R F E V S S S L I L R N H N E P F L D R I V T C D E K W I L Y D N R R R S A Q W L D Q E E A P K H F P K P I L H P K K V M V T I W W S A A G L I H Y S F L N P G E T I T S E K Y A Q E I D E M N Q K L Q R L Q L A L V N R K G P I L L H D N A R P H V A Q P T L Q K L N E L G Y E V L P H P P Y S P D L L P T N Y H V F K H L N N F L Q G K R F H N Q Q D A E N A F Q E F V E S Q S T D F Y A T G I N Q L I S R W Q K C V D C N G S Y F D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Has also kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity (PubMed:16332963, PubMed:16672366, PubMed:17403897, PubMed:17877369, PubMed:18263876, PubMed:20521842, PubMed:22231448, PubMed:24573677). In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining (PubMed:16332963, PubMed:21187428, PubMed:22231448). Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A (PubMed:18790802, PubMed:20457750).
Alternative names
Histone-lysine N-methyltransferase SETMAR, SET domain and mariner transposase fusion protein, Metnase, SETMAR
Recommended products
Recombinant Human Metnase protein is a Human Fragment protein, in the 27 to 684 aa range, expressed in Mammalian, with >=30% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=30% SDS-PAGE
- Expression system
- Mammalian
- Tags
- DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- No
- Accession
- Q53H47-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- Q L D V A C G Q E N L P V G A W P P G A A P A P F Q Y T P D H V V G P G A D I D P T Q I T F P G C I C V K T P C L P G T C S C L R H G E N Y D D N S C L R D I G S G G K Y A E P V F E C N V L C R C S D H C R N R V V Q K G L Q F H F Q V F K T H K K G W G L R T L E F I P K G R F V C E Y A G E V L G F S E V Q R R I H L Q T K S D S N Y I I A I R E H V Y N G Q V M E T F V D P T Y I G N I G R F L N H S C E P N L L M I P V R I D S M V P K L A L F A A K D I V P E E E L S Y D Y S G R Y L N L T V S E D K E R L D H G K L R K P C Y C G A K S C T A F L P F D S S L Y C P V E K S N I S C G N E K E P S M C G S A P S V F P S C K R L T L E T M K M M L D K K Q I R A I F L F E F K M G R K A A E T T R N I N N A F G P G T A N E R T V Q W W F K K F C K G D E S L E D E E R S G R P S E V D N D Q L R A I I E A D P L T T T R E V A E E L N V N H S T V V R H L K Q I G K V K K L D K W V P H E L T E N Q K N R R F E V S S S L I L R N H N E P F L D R I V T C D E K W I L Y D N R R R S A Q W L D Q E E A P K H F P K P I L H P K K V M V T I W W S A A G L I H Y S F L N P G E T I T S E K Y A Q E I D E M N Q K L Q R L Q L A L V N R K G P I L L H D N A R P H V A Q P T L Q K L N E L G Y E V L P H P P Y S P D L L P T N Y H V F K H L N N F L Q G K R F H N Q Q D A E N A F Q E F V E S Q S T D F Y A T G I N Q L I S R W Q K C V D C N G S Y F D
- Accession
- Q53H47
- Protein length
- Fragment
- Predicted molecular weight
- 76 kDa
- Amino acids
- 27 to 684
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Has also kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity (PubMed:16332963, PubMed:16672366, PubMed:17403897, PubMed:17877369, PubMed:18263876, PubMed:20521842, PubMed:22231448, PubMed:24573677). In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining (PubMed:16332963, PubMed:21187428, PubMed:22231448). Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A (PubMed:18790802, PubMed:20457750).
- Sequence similarities
In the N-terminal section; belongs to the class V-like SAM-binding methyltransferase superfamily.
- Post-translational modifications
Methylated. Methylation regulates activity in DNA decatenation.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
The protein fragment was expressed in FreeStyle 293-F cells.
Previously labelled as SETMAR.
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2 product images
SDS-PAGE - Recombinant Human Metnase protein (ab196433)
SDS-PAGE analysis of 2 μg of ab196433 on 10% SDS-PAGE gel stained with Coomassie.
SDS-PAGE - Recombinant Human Metnase protein (ab196433)
SDS-PAGE analysis of 3 μg of ab196433 on 10% SDS-PAGE gel stained with Coomassie.
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