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AB187477

Recombinant Human METTL21D protein (His tag N-Terminus)

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(1 Publication)

Recombinant Human METTL21D protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 229 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C14orf138, METTL21D, VCPKMT, Protein N-lysine methyltransferase METTL21D, Methyltransferase-like protein 21D, VCP lysine methyltransferase, Valosin-containing protein lysine methyltransferase, VCP-KMT

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9H867

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMADTLESSLEDPLRSFVRVLEKRDGTVLRLQQYSSGGVGCVVWDAAIVLSKYLETPEFSGDGAHALSRRSVLELGSGTGAVGLMAATLGADVVVTDLEELQDLLKMNINMNKHLVTGSVQAKVLKWGEEIEGFPSPPDFILMADCIYYEESLEPLLKTLKDISGFETCIICCYEQRTMGKNPEIEKKYFELLQLDFDFEKIPLEKHDEEYRSEDIHIIYIRKKKSKFPS","proteinLength":"Full Length","predictedMolecularWeight":"28.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":229,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H867","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

METTL21D also known as EF2-KMT or methyltransferase-like protein 21D is an enzyme with a molecular mass of approximately 33 kDa. It belongs to the protein lysine methyltransferase family and primarily acts on eukaryotic elongation factor 2 (eEF2) adding methyl groups to lysine residues affecting protein function. METTL21D expression is observed in various tissues including the brain and skeletal muscle indicating its widespread functional importance.
Biological function summary

METTL21D influences protein synthesis through its methyltransferase activity. It modifies eEF2 which is essential for the elongation phase during protein translation. By modifying eEF2 METTL21D impacts protein translation rates and cellular protein homeostasis. While not part of a larger complex METTL21D's interactions with other proteins highlight its regulatory function in translation.

Pathways

Protein translation and cellular metabolism feature prominently in METTL21D's activity. Its modification of eEF2 integrates METTL21D into the translation pathway influencing global protein synthesis. METTL21D also connects to pathways involving protein quality control where it potentially interacts with other methyltransferases that regulate cellular stress responses.

METTL21D links to conditions such as neurodegenerative diseases and certain types of cancer. Alterations in its expression or function can disrupt protein homeostasis contributing to disease progression. METTL21D through its interaction with eEF2 and possibly other methyltransferases may therefore affect pathways that lead to the development of these disorders providing insights for potential therapeutic interventions.
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Specifications

Form

Liquid

Additional notes

ab187477 was purified using conventional chromatography techniques.

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General info

Function

Protein N-lysine methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity.

Sequence similarities

Belongs to the methyltransferase superfamily. METTL21 family.

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Product protocols

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Target data

Protein N-lysine methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity.
See full target information VCPKMT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neuron 110:1944-1958.e8 PubMed35421327

2022

Functional coupling of TRPM2 and extrasynaptic NMDARs exacerbates excitotoxicity in ischemic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyu Zong,Jianlin Feng,Zhichao Yue,Yunfeng Li,Gongxiong Wu,Baonan Sun,Yanlin He,Barbara Miller,Albert S Yu,Zhongping Su,Jia Xie,Yasuo Mori,Bing Hao,Lixia Yue
View all publications
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