Recombinant Human METTL7A protein is a Human Full Length protein, in the 1 to 244 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M E L T I F I L R L A I Y I L T F P L Y L L N F L G L W S W I C K K W F P Y F L V R F T V I Y N E Q M A S K K R E L F S N L Q E F A G P S G K L S L L E V G C G T G A N F K F Y P P G C R V T C I D P N P N F E K F L I K S I A E N R H L Q F E R F V V A A G E N M H Q V A D G S V D V V V C T L V L C S V K N Q E R I L R E V C R V L R P G G A F Y F M E H V A A E C S T W N Y F W Q Q V L D P A W H L L F D G C N L T R E S W K A L E R A S F S K L K L Q H I Q A P L S W E L V R P H I Y G Y A V K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Thiol S-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to alkyl and phenolic thiol-containing acceptor substrates. Together with TMT1B accounts for most of S-thiol methylation activity in the endoplasmic reticulum of hepatocytes (PubMed:37137720). Able to methylate the N6 position of adenosine residues in long non-coding RNAs (lncRNAs). May facilitate lncRNAs transfer into exosomes at the tumor-stroma interface (PubMed:34980213). Promotes osteogenic and odontogenic differentiation by regulating the expression of genes involved in stem cell differentiation and survival (PubMed:34226523, PubMed:34790668). Targeted from the endoplasmic reticulum to lipid droplets, where it recruits cellular proteins to form functional organelles (PubMed:19773358). (Microbial infection) May be involved in the assembly and release stages of hepatitis C virus (HCV) life cycle and thus play a crucial role in HCV propagation.
Alternative names
METTL7A, PRO0066, UNQ1902/PRO4348, TMT1A, Thiol S-methyltransferase TMT1A, Methyltransferase-like protein 7A, N6-adenosine-methyltransferase TMT1A, Protein AAM-B, Thiol methyltransferase 1A
Recommended products
Recombinant Human METTL7A protein is a Human Full Length protein, in the 1 to 244 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9H8H3-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M E L T I F I L R L A I Y I L T F P L Y L L N F L G L W S W I C K K W F P Y F L V R F T V I Y N E Q M A S K K R E L F S N L Q E F A G P S G K L S L L E V G C G T G A N F K F Y P P G C R V T C I D P N P N F E K F L I K S I A E N R H L Q F E R F V V A A G E N M H Q V A D G S V D V V V C T L V L C S V K N Q E R I L R E V C R V L R P G G A F Y F M E H V A A E C S T W N Y F W Q Q V L D P A W H L L F D G C N L T R E S W K A L E R A S F S K L K L Q H I Q A P L S W E L V R P H I Y G Y A V K
- Accession
- Q9H8H3
- Protein length
- Full Length
- Amino acids
- 1 to 244
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Thiol S-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to alkyl and phenolic thiol-containing acceptor substrates. Together with TMT1B accounts for most of S-thiol methylation activity in the endoplasmic reticulum of hepatocytes (PubMed:37137720). Able to methylate the N6 position of adenosine residues in long non-coding RNAs (lncRNAs). May facilitate lncRNAs transfer into exosomes at the tumor-stroma interface (PubMed:34980213). Promotes osteogenic and odontogenic differentiation by regulating the expression of genes involved in stem cell differentiation and survival (PubMed:34226523, PubMed:34790668). Targeted from the endoplasmic reticulum to lipid droplets, where it recruits cellular proteins to form functional organelles (PubMed:19773358).
- Sequence similarities
Belongs to the methyltransferase superfamily.
- Post-translational modifications
Methylated at lysine residues most likely by EZH2.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
METTL7A also known as Methyltransferase-Like Protein 7A is an enzyme with a mass of approximately 24 kDa. It belongs to the methyltransferase family which affects the transfer of methyl groups to various substrates including proteins DNA and RNA. Expression of METTL7A occurs widely in human tissues with notable presence in the liver lung and adipose tissue. These characteristics suggest this protein plays a versatile role in diverse cellular processes across multiple systems in the body.
- Biological function summary
METTL7A is important for maintaining cellular homeostasis and has a role in lipid metabolism. Although details about its association with specific protein complexes are limited METTL7A influences processes like lipid droplet formation and energy balance. Its expression levels indicate that it could affect the regulation of lipid accumulation and alterations may impact metabolic functions.
- Pathways
Research reveals that METTL7A is involved in metabolic pathways pertinent to lipid and energy regulation. The protein is a part of the lipid metabolism pathway interacting with other proteins like PNPLA3 which is significant in lipid droplet biology. METTL7A interactions influence how cells store and manage lipids highlighting its importance in maintaining lipid equilibrium in cells.
- Associated diseases and disorders
METTL7A shows a connection to liver diseases and metabolic syndromes. Variations in METTL7A expression are linked to non-alcoholic fatty liver disease (NAFLD) where alterations in lipid droplet metabolism impact liver health. Relations with proteins like adiponectin suggest its involvement in broader metabolic conditions including obesity-related disorders. Consequently METTL7A remains a focus in studies aiming to comprehend and potentially treat these metabolic diseases.
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1 product image
SDS-PAGE - Recombinant Human METTL7A protein (ab161809)
ab161809 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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