Recombinant Human MFAP4 protein (His tag) is a Human Full Length protein, in the 22 to 255 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D L V S G I R G D A L E R F C L Q Q P L D C D D I Y A Q G Y Q S D G V Y L I Y P S G P S V P V P V F C D M T T E G G K W T V F Q K R F N G S V S F F R G W N D Y K L G F G R A D G E Y W L G L Q N M H L L T L K Q K Y E L R V D L E D F E N N T A Y A K Y A D F S I S P N A V S A E E D G Y T L F V A G F E D G G A G D S L S Y H S G Q K F S T F D R D Q D L F V Q N C A A L S S G A F W F R S C H F A N L N G F Y L G G S H L S Y A N G I N W A Q W K G F Y Y S L K R T E M K I R R A H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Could be involved in calcium-dependent cell adhesion or intercellular interactions. May contribute to the elastic fiber assembly and/or maintenance (PubMed:26601954).
Alternative names
Microfibril-associated glycoprotein 4, MFAP4
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Recombinant Human MFAP4 protein (His tag) is a Human Full Length protein, in the 22 to 255 aa range, expressed in Baculovirus infected insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P55083-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D L V S G I R G D A L E R F C L Q Q P L D C D D I Y A Q G Y Q S D G V Y L I Y P S G P S V P V P V F C D M T T E G G K W T V F Q K R F N G S V S F F R G W N D Y K L G F G R A D G E Y W L G L Q N M H L L T L K Q K Y E L R V D L E D F E N N T A Y A K Y A D F S I S P N A V S A E E D G Y T L F V A G F E D G G A G D S L S Y H S G Q K F S T F D R D Q D L F V Q N C A A L S S G A F W F R S C H F A N L N G F Y L G G S H L S Y A N G I N W A Q W K G F Y Y S L K R T E M K I R R A H H H H H H
- Accession
- P55083
- Protein length
- Full Length
- Predicted molecular weight
- 27.5 kDa
- Amino acids
- 22 to 255
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Could be involved in calcium-dependent cell adhesion or intercellular interactions. May contribute to the elastic fiber assembly and/or maintenance (PubMed:26601954).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MFAP4 also known as Microfibril-Associated Glycoprotein 4 is a protein that plays a significant role in the extracellular matrix architecture. The molecular mass of MFAP4 is about 29 kDa. This protein is expressed in various tissues including the lung liver heart and blood vessels. As a part of the extracellular matrix MFAP4 contributes to the structural integrity and elasticity of connective tissues.
- Biological function summary
MFAP4 interacts with components of the microfibrillar network. It is not a standalone entity but functions as part of a larger complex that includes elastin and microfibrillin-1. This association enhances the mechanical properties of tissues maintaining them against pressure and stretch forces. MFAP4 also participates in cellular responses such as cell adhesion and migration which are critical for tissue remodeling and repair processes.
- Pathways
MFAP4 engages in multiple biological pathways related to the extracellular matrix and tissue homeostasis. The TGF-beta signaling pathway is notable as MFAP4 interacts with multiple proteins within this pathway such as fibrillin-1. Through these interactions MFAP4 influences the regulation of cell growth and differentiation. Another pathway is the integrin signaling pathway where its association with integrins supports cell adhesion and communication within the extracellular matrix.
- Associated diseases and disorders
MFAP4 shows associations with fibrosis and chronic obstructive pulmonary disease (COPD). Both conditions involve alterations in the extracellular matrix composition and function. In fibrosis the dysregulated expression of MFAP4 can affect connective tissue remodeling. Altered MFAP4 levels have also been observed in COPD where it may impact lung tissue elasticity. The protein connects with TGF-beta in the context of these diseases influencing abnormal tissue remodeling and inflammation processes.
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1 product image
SDS-PAGE - Recombinant Human MFAP4 protein (His tag) (ab219453)
15% SDS-PAGE using 3ug of ab219453. Molecular Weight: 27.5kDa; 28-40KDa (SDS-PAGE under reducing conditions.)
Lane 1: Reduced form
Lane 2: Non-Reduced form
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