JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB153557

Recombinant Human MFSD2A/NLS1 protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human MFSD2A/NLS1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 530 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

MFSD2, NLS1, HMFN0656, PP9177, UNQ300/PRO341, MFSD2A, Sodium-dependent lysophosphatidylcholine symporter 1, Sodium-dependent LPC symporter 1, Major facilitator superfamily domain-containing protein 2A, HsMFSD2A, MFSD2a

1 Images
SDS-PAGE - Recombinant Human MFSD2A/NLS1 protein (GST tag N-Terminus) (AB153557)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MFSD2A/NLS1 protein (GST tag N-Terminus) (AB153557)

ab153557 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q8NA29

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB259318

Anti-Aquaporin 4 antibody [EPR24281-65]

BOND RX™ Validated|KO Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aquaporin 4 antibody [EPR24281-65] (AB259318)

  • 0
  • 0
Primary Antibodies

AB307690

Anti-MFSD2A/NLS1 antibody [EPR24503-101]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-MFSD2A/NLS1 antibody [EPR24503-101] (AB307690)

  • 0
  • 0
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 20
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product was previously labelled as MFSD2A.
style
left-column

Sequence info

[{"sequence":"MAKGEGAESGSAAGLLPTSILQSTERPAQVKKEPKKKKQQLSVCNKLCYALGGAPYQVTGCALGFFLQIYLLDVAQVGPFSASIILFVGRAWDAITDPLVGLCISKSPWTCLGRLMPWIIFSTPLAVIAYFLIWFVPDFPHGQTYWYLLFYCLFETMVTCFHVPYSALTMFISTEQTERDSATAYRMTVEVLGTVLGTAIQGQIVGQADTPCFQDLNSSTVASQSANHTHGTTSHRETQKAYLLAAGVIVCIYIICAVILILGVREQREPYEAQQSEPIAYFRGLRLVMSHGPYIKLITGFLFTSLAFMLVEGNFVLFCTYTLGFRNEFQNLLLAIMLSATLTIPIWQWFLTRFGKKTAVYVGISSAVPFLILVALMESNLIITYAVAVAAGISVAAAFLLPWSMLPDVIDDFHLKQPHFHGTEPIFFSFYVFFTKFASGVSLGISTLSLDFAGYQTRGCSQPERVKFTLNMLVTMAPIVLILLGLLLFKMYPIDEERRRQNKKALQALRDEASSSGCSETDSTELASIL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":530,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8NA29","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MFSD2A also known as Major Facilitator Superfamily Domain-Containing Protein 2A functions as a transporter protein. It efficiently facilitates transport of specific lipids across cellular membranes. This protein has a mass of approximately 56 kDa and is mainly expressed in the blood-brain barrier and endothelial cells. MFSD2A's expression in these areas plays an important role in transport through the protective barrier in the brain.
Biological function summary

MFSD2A is important in mediating uptake of omega-3 fatty acids such as docosahexaenoic acid (DHA) into the brain. It achieves this by ensuring proper distribution of lipids necessary for maintaining brain health and function. While not known to be a part of a larger complex MFSD2A works closely with lipid molecules and other membrane transporters to regulate lipid homeostasis within the brain.

Pathways

MFSD2A is involved in the lipid metabolic pathway and the neurovascular signaling pathway. Its activity significantly affects how lipids are transported and utilized within these systems. It interacts functionally with proteins like ABC transporters which also contribute to lipid processing and transport. These pathways highlight the importance of MFSD2A in lipid utilization and brain metabolism.

MFSD2A is closely linked to neurological conditions such as microcephaly and certain neurodevelopmental disorders. Its malfunction or altered expression can disrupt the supply of essential lipids to the brain leading to developmental abnormalities. The protein's role connects to other transporters like FABP5 hinting at a network of lipid transport mechanisms that when altered contribute to these diseases.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Sodium-dependent lysophosphatidylcholine (LPC) symporter, which plays an essential role for blood-brain barrier formation and function (PubMed : 24828040, PubMed : 32572202, PubMed : 34135507). Specifically expressed in endothelium of the blood-brain barrier of micro-vessels and transports LPC into the brain (By similarity). Transport of LPC is essential because it constitutes the major mechanism by which docosahexaenoic acid (DHA), an omega-3 fatty acid that is essential for normal brain growth and cognitive function, enters the brain (PubMed : 26005868, PubMed : 34135507). Transports LPC carrying long-chain fatty acids such LPC oleate and LPC palmitate with a minimum acyl chain length of 14 carbons (By similarity). Does not transport docosahexaenoic acid in unesterified fatty acid (By similarity). Specifically required for blood-brain barrier formation and function, probably by mediating lipid transport (By similarity). Not required for central nervous system vascular morphogenesis (By similarity). Acts as a transporter for tunicamycin, an inhibitor of asparagine-linked glycosylation (PubMed : 21677192). In placenta, acts as a receptor for ERVFRD-1/syncytin-2 and is required for trophoblast fusion (PubMed : 18988732, PubMed : 23177091).

Sequence similarities

Belongs to the major facilitator superfamily.

style
left-column

Product protocols

style
left-column

Target data

Sodium-dependent lysophosphatidylcholine (LPC) symporter, which plays an essential role for blood-brain barrier formation and function (PubMed : 24828040, PubMed : 32572202, PubMed : 34135507). Specifically expressed in endothelium of the blood-brain barrier of micro-vessels and transports LPC into the brain (By similarity). Transport of LPC is essential because it constitutes the major mechanism by which docosahexaenoic acid (DHA), an omega-3 fatty acid that is essential for normal brain growth and cognitive function, enters the brain (PubMed : 26005868, PubMed : 34135507). Transports LPC carrying long-chain fatty acids such LPC oleate and LPC palmitate with a minimum acyl chain length of 14 carbons (By similarity). Does not transport docosahexaenoic acid in unesterified fatty acid (By similarity). Specifically required for blood-brain barrier formation and function, probably by mediating lipid transport (By similarity). Not required for central nervous system vascular morphogenesis (By similarity). Acts as a transporter for tunicamycin, an inhibitor of asparagine-linked glycosylation (PubMed : 21677192). In placenta, acts as a receptor for ERVFRD-1/syncytin-2 and is required for trophoblast fusion (PubMed : 18988732, PubMed : 23177091).
See full target information MFSD2A
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com