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AB132761

Recombinant Human MG53 protein (GST tag N-Terminus)

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Recombinant Human MG53 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 268 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

MG53, TRIM72, Tripartite motif-containing protein 72, Mitsugumin-53, Mg53

1 Images
SDS-PAGE - Recombinant Human MG53 protein (GST tag N-Terminus) (AB132761)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MG53 protein (GST tag N-Terminus) (AB132761)

ab132761 on 12.5% SDS-PAGE stained with Coomassie blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

Q6ZMU5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB307593

Anti-MG53 antibody [EPR26057-7]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Frozen sections) - Anti-MG53 antibody [EPR26057-7] (AB307593)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15
Primary Antibodies

AB236451

Anti-TIPIN antibody

Immunocytochemistry/ Immunofluorescence - Anti-TIPIN antibody (AB236451)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as TRIM72.
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Sequence info

[{"sequence":"MSAAPGLLHQELSCPLCLQLFDAPVTAECGHSFCRACLGRVAGEPAADGTVLCPCCQAPTRPQALSTNLQLARLVEGLAQVPQGHCEEHLDPLSIYCEQDRALVCGVCASLGSHRGHRLLPAAEAHARLKTQLPQQKLQLQEACMRKEKSVAVLEHQLVEVEETVRQFRGAVGEQLGKMRVFLAALEGSLDREAERVRGEAGVALRRELGSLNSYLEQLRQMEKVLEEVADKPQTEFLMKYCLVTSRLQKILAESPPPARLDIQLPIIS","proteinLength":"Full Length","predictedMolecularWeight":"55.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":268,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q6ZMU5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MG53 also known as TRIM72 is a protein that plays an important role in membrane repair. This protein has a molecular mass of approximately 72 kDa and is classified as a member of the tripartite motif-containing (TRIM) protein family. MG53 is expressed highly in striated muscles including the cardiac and skeletal muscles. Its mechanical action involves sensing membrane disruptions and facilitating the repair process by recruiting vesicles to the site of injury.
Biological function summary

MG53 acts as a critical factor in maintaining cellular membrane integrity. It forms part of a complex network where it interacts with other proteins to orchestrate the membrane repair process. MG53 functions as a scaffold bringing together various repair components and coordinating their activity to mend damaged membranes. This repair process is essential for muscle cells which frequently experience mechanical stress.

Pathways

MG53 integrates into cellular repair pathways and is pivotal in the response to membrane damage. It interacts with important pathways like the IRS1/PI3K/AKT signaling pathway where it modulates cellular responses to stress and injury. MG53 also associates with other proteins such as caveolin-3 assisting in stabilizing the membrane during injury response.

MG53 has relevance to conditions like muscular dystrophy and cardiomyopathy. Mutations or dysregulation of MG53 can lead to inadequate membrane repair contributing to muscle weakness and cardiac dysfunction. In muscular disorders MG53 interacts with dysferlin another repair protein to support membrane stabilization and disruptions in this interaction exacerbate disease progression.
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Specifications

Form

Liquid

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General info

Function

Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites (PubMed : 36944613). Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3 (By similarity). Acts as a sensor of oxidation : upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site (By similarity). This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation (By similarity). Probably acts upstream of the Ca(2+)-dependent membrane resealing process (By similarity). Required for transport of DYSF to sites of cell injury during repair patch formation (By similarity). Regulates membrane budding and exocytosis (By similarity). May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).

Sequence similarities

Belongs to the TRIM/RBCC family.

Post-translational modifications

Disulfide bond formation at Cys-242 occurs in case of membrane damage that cause the entry of the oxidized milieu of the extracellular space, resulting in homooligomerization.. S-nitrosylation at Cys-144 stabilizes TRIM72 and protects against oxidation-induced protein degradation and cell death.

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Product protocols

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Target data

Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites (PubMed : 36944613). Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3 (By similarity). Acts as a sensor of oxidation : upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site (By similarity). This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation (By similarity). Probably acts upstream of the Ca(2+)-dependent membrane resealing process (By similarity). Required for transport of DYSF to sites of cell injury during repair patch formation (By similarity). Regulates membrane budding and exocytosis (By similarity). May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).
See full target information TRIM72
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Product promise

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