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AB177615

Recombinant Human MGAT2 protein (denatured) (His tag N-Terminus)

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Recombinant Human MGAT2 protein (denatured) (His tag N-Terminus) is a Human Fragment protein, in the 30 to 447 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

GlcNAc-T II, Mannoside acetylglucosaminyltransferase 2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, GNT-II, MGAT2

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SDS-PAGE - Recombinant Human MGAT2 protein (denatured) (His tag N-Terminus) (AB177615)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MGAT2 protein (denatured) (His tag N-Terminus) (AB177615)

15% SDS-PAGE analysis of ab177615 (3μg)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q10469

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMRQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLTLRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRPSTQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ","proteinLength":"Fragment","predictedMolecularWeight":"50 kDa","actualMolecularWeight":null,"aminoAcidEnd":447,"aminoAcidStart":30,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q10469","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGAT2 also known as alpha-16-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase is an enzyme with a molecular mass of approximately 58 kDa. It plays an important role in the glycosylation process. We find MGAT2 mainly expressed in the Golgi apparatus within various human tissues. This enzyme catalyzes the addition of N-acetylglucosamine to form branched N-glycans which are essential for protein maturation and function.
Biological function summary

MGAT2 participates in the modification of N-linked oligosaccharides as part of a larger glycosylation complex. Glycosylation is a critical step in the maturation of glycoproteins impacting cell signaling protein folding and stability. MGAT2 modifies high mannose-type oligosaccharides into complex types enhancing their functional diversity. The role of MGAT2 in these processes affects multiple cellular functions necessary for maintaining homeostasis.

Pathways

MGAT2 interacts with key proteins in the N-glycan biosynthesis pathway and has connections to the calnexin/calreticulin cycle. In these pathways MGAT2 works with other glycosyltransferases to construct fully mature glycoproteins which are subsequently important for protein folding and quality control. Its interaction with proteins such as MGAT1 and GNPTAB ensures efficient glycoprotein processing and quality control before they achieve their final functional structures.

MGAT2 has associations with carbohydrate-deficient glycoprotein syndrome type II a rare inherited disorder affecting glycosylation. This condition results from mutations in the MGAT2 gene leading to improper glycan branching and subsequent physiological issues. The altered glycosylation impacts various proteins contributing to this multisystem disorder. Additionally variations and malfunctions involving MGAT2 can affect immune response linking it indirectly to immunological conditions although more research is necessary to fully comprehend these connections.
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Specifications

Form

Liquid

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General info

Function

Plays an essential role in protein N-glycosylation. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) onto the free terminal mannose moiety in the core structure of the nascent N-linked glycan chain, giving rise to the second branch in complex glycans.

Sequence similarities

Belongs to the glycosyltransferase 16 (GT16) protein family.

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Product protocols

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Target data

Plays an essential role in protein N-glycosylation. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) onto the free terminal mannose moiety in the core structure of the nascent N-linked glycan chain, giving rise to the second branch in complex glycans.
See full target information MGAT2
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