JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB276387

Recombinant Human MGAT5 protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human MGAT5 protein (His tag) is a Human Fragment protein, in the 189 to 741 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

GGNT5, MGAT5, GlcNAc-T V, Mannoside acetylglucosaminyltransferase 5, N-acetylglucosaminyl-transferase V, GNT-V

1 Images
SDS-PAGE - Recombinant Human MGAT5 protein (His tag) (AB276387)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MGAT5 protein (His tag) (AB276387)

SDS-PAGE analysis of ab276387

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q09328

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 100% PBS

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"LSEVENWCPHLPWRAKNPYEEADHNSLAEIRTDFNILYSMMKKHEEFRWMRLRIRRMADAWIQAIKSLAEKQNLEKRKRKKVLVHLGLLTKESGFKIAETAFSGGPLGELVQWSDLITSLYLLGHDIRISASLAELKEIMKKVVGNRSGCPTVGDRIVELIYIDIVGLAQFKKTLGPSWVHYQCMLRVLDSFGTEPEFNHANYAQSKGHKTPWGKWNLNPQQFYTMFPHTPDNSFLGFVVEQHLNSSDIHHINEIKRQNQSLVYGKVDSFWKNKKIYLDIIHTYMEVHATVYGSSTKNIPSYVKNHGILSGRDLQFLLRETKLFVGLGFPYEGPAPLEAIANGCAFLNPKFNPPKSSKNTDFFIGKPTLRELTSQHPYAEVFIGRPHVWTVDLNNQEEVEDAVKAILNQKIEPYMPYEFTCEGMLQRINAFIEKQDFCHGQVMWPPLSALQVKLAEPGQSCKQVCQESQLICEPSFFQHLNKDKDMLKYKVTCQSSELAKDILVPSFDPKNKHCVFQGDLLLFSCAGAHPRHQRVCPCRDFIKGQVALCKDCL","proteinLength":"Fragment","predictedMolecularWeight":"65 kDa","actualMolecularWeight":null,"aminoAcidEnd":741,"aminoAcidStart":189,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q09328","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGAT5 also known as beta-16-N-acetylglucosaminyltransferase V or GnT-V is an enzyme with a molecular mass of approximately 83 kDa. This enzyme plays a role in the Golgi apparatus where it modifies N-glycans on glycoproteins by adding N-acetylglucosamine to N-glycans. MGAT5 is expressed in various tissues including the brain liver and kidneys. Its enzymatic function is essential in creating complex branched glycan structures influencing the cellular surface glycosylation patterns.
Biological function summary

MGAT5 contributes to cellular processes such as cell-cell communication cell migration and signal transduction. These processes are important in tissues where it is expressed. MGAT5 is not part of a complex but works closely with other glycosyltransferases in the glycosylation pathway. Its glycan products interact with lectins and receptors on the cell surface influencing signaling pathways and cellular behavior.

Pathways

MGAT5 is significant in the glycosylation pathway and the PI3K/AKT signaling pathway. In these pathways MGAT5 modifies glycoproteins which can impact receptor function and downstream signaling. It interacts with proteins such as integrins and growth factor receptors affecting cellular responses to environmental cues and influencing growth and differentiation signals.

MGAT5 is associated with cancer progression and autoimmune diseases. Altered MGAT5 expression and activity in cancer cells can promote tumor growth and metastasis by enhancing cell invasion and evasion mechanisms. The enzyme links to proteins like TGF-beta receptor and E-cadherin in cancer contributing to the changes in cellular adhesion and signaling that facilitate metastasis. In autoimmune diseases MGAT5 modulates immune cell function impacting disease progression and severity.
style
left-column

Specifications

Form

Lyophilized

style
left-column

General info

Function

Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides (PubMed : 10395745, PubMed : 30140003). Catalyzes an important step in the biosynthesis of branched, complex-type N-glycans, such as those found on EGFR, TGFR (TGF-beta receptor) and CDH2 (PubMed : 10395745, PubMed : 22614033, PubMed : 30140003). Via its role in the biosynthesis of complex N-glycans, plays an important role in the activation of cellular signaling pathways, reorganization of the actin cytoskeleton, cell-cell adhesion and cell migration. MGAT5-dependent EGFR N-glycosylation enhances the interaction between EGFR and LGALS3 and thereby prevents rapid EGFR endocytosis and prolongs EGFR signaling. Required for efficient interaction between TGFB1 and its receptor. Enhances activation of intracellular signaling pathways by several types of growth factors, including FGF2, PDGF, IGF, TGFB1 and EGF. MGAT5-dependent CDH2 N-glycosylation inhibits CDH2-mediated homotypic cell-cell adhesion and contributes to the regulation of downstream signaling pathways. Promotes cell migration. Contributes to the regulation of the inflammatory response. MGAT5-dependent TCR N-glycosylation enhances the interaction between TCR and LGALS3, limits agonist-induced TCR clustering, and thereby dampens TCR-mediated responses to antigens. Required for normal leukocyte evasation and accumulation at sites of inflammation (By similarity). Inhibits attachment of monocytes to the vascular endothelium and subsequent monocyte diapedesis (PubMed : 22614033).. Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A. Promotes proliferation of umbilical vein endothelial cells and angiogenesis, at least in part by promoting the release of the growth factor FGF2 from the extracellular matrix.

Sequence similarities

Belongs to the glycosyltransferase 18 family.

Post-translational modifications

N-glycosylated.. A secreted form is released from the membrane after cleavage by gamma-secretase.

style
left-column

Product protocols

style
left-column

Target data

Catalyzes the addition of N-acetylglucosamine (GlcNAc) in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides (PubMed : 10395745, PubMed : 30140003). Catalyzes an important step in the biosynthesis of branched, complex-type N-glycans, such as those found on EGFR, TGFR (TGF-beta receptor) and CDH2 (PubMed : 10395745, PubMed : 22614033, PubMed : 30140003). Via its role in the biosynthesis of complex N-glycans, plays an important role in the activation of cellular signaling pathways, reorganization of the actin cytoskeleton, cell-cell adhesion and cell migration. MGAT5-dependent EGFR N-glycosylation enhances the interaction between EGFR and LGALS3 and thereby prevents rapid EGFR endocytosis and prolongs EGFR signaling. Required for efficient interaction between TGFB1 and its receptor. Enhances activation of intracellular signaling pathways by several types of growth factors, including FGF2, PDGF, IGF, TGFB1 and EGF. MGAT5-dependent CDH2 N-glycosylation inhibits CDH2-mediated homotypic cell-cell adhesion and contributes to the regulation of downstream signaling pathways. Promotes cell migration. Contributes to the regulation of the inflammatory response. MGAT5-dependent TCR N-glycosylation enhances the interaction between TCR and LGALS3, limits agonist-induced TCR clustering, and thereby dampens TCR-mediated responses to antigens. Required for normal leukocyte evasation and accumulation at sites of inflammation (By similarity). Inhibits attachment of monocytes to the vascular endothelium and subsequent monocyte diapedesis (PubMed : 22614033).. Secreted alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A. Promotes proliferation of umbilical vein endothelial cells and angiogenesis, at least in part by promoting the release of the growth factor FGF2 from the extracellular matrix.
See full target information MGAT5
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB87977

Anti-MGAT5 antibody [3E9]

Flow Cytometry - Anti-MGAT5 antibody [3E9] (AB87977)

  • 4
  • 5
Primary Antibodies

AB103427

Anti-MGAT3 antibody

Western blot - Anti-MGAT3 antibody (AB103427)

  • 4
  • 4
Proteins & Peptides

AB171695

Recombinant Human LRRC59 protein

SDS-PAGE - Recombinant Human LRRC59 protein (AB171695)

  • 0
  • 0
Proteins & Peptides

AB161287

Recombinant Human Keratocan/KTN protein

SDS-PAGE - Recombinant Human Keratocan/KTN protein (AB161287)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com