Recombinant Human MGP protein (denatured) is a Human Full Length protein, in the 20 to 96 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Publications
Filter by
- Arthritis research & therapy 24:442022Chondrocyte-derived exosomes promote cartilage calcification in temporomandibular joint osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Liu,Ruoxin Wang,Shujie Hou,Feng He,Yuanjun Ma,Tao Ye,Shibin Yu,Hongwei Chen,Helin Wang,Mian ZhangPubMed 35164837
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M Y E S H E S M E S Y E L N P F I N R R N A N T F I S P Q Q R W R A K V Q E R I R E R S K P V H E L N R E A C D D Y R L C E R Y A M V Y G Y N A A Y N R Y F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
Alternative names
Matrix Gla protein, MGP, Cell growth-inhibiting gene 36 protein, GIG36, MGLAP, MGP
Recommended products
Recombinant Human MGP protein (denatured) is a Human Full Length protein, in the 20 to 96 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P08493-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M Y E S H E S M E S Y E L N P F I N R R N A N T F I S P Q Q R W R A K V Q E R I R E R S K P V H E L N R E A C D D Y R L C E R Y A M V Y G Y N A A Y N R Y F
- Accession
- Protein length
Full Length
- Predicted molecular weight
11.8 kDa
- Amino acids
20 to 96
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab113156 was purified using conventional chromatography techniques.
General info
- Function
Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
- Sequence similarities
Belongs to the osteocalcin/matrix Gla protein family.
- Post-translational modifications
Requires vitamin K-dependent gamma-carboxylation for its function.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MGP protein also known as Matrix Gla Protein is a secreted protein that has a molecular mass of approximately 10 kDa. It is expressed in the cartilage bone and vasculature where it plays a significant role in inhibiting calcification. MGP undergoes gamma-carboxylation a post-translational modification that is important for its function in the extracellular matrix. This modification allows MGP to bind calcium ions preventing mineral deposition in the vascular tissues. Alongside its role in bone health MGP acts as an antagonist against vascular calcification.
- Biological function summary
MGP regulates extracellular matrix mineralization playing an important part in maintaining tissue integrity. It is not part of a simple complex structure but interacts with other extracellular matrix components like osteopontin. The function of MGP in bone and tissue health highlights its protective roles against pathological calcification. Defective or insufficient MGP leads to increased calcification contributing to its important role in keeping arteries flexible and functional.
- Pathways
MGP interacts within the BMP signaling pathway where it modulates bone morphogenetic proteins to prevent inappropriate ossification. It also participates in the regulation of the vitamin K-dependent carboxylation pathway critical for activating MGP's calcification inhibitory function. Related proteins in the pathways include osteocalcin which also undergoes similar gamma-carboxylation and fetuin-A another calcification inhibitor. MGP's effect on these pathways showcases its pivotal role in tissue and vascular health.
- Associated diseases and disorders
MGP has connections to arteriosclerosis and Keutel syndrome where abnormal calcification is a common feature. In arteriosclerosis MGP deficiency can lead to inappropriate calcification of the arteries complicating cardiovascular health. Keutel syndrome a rare genetic disorder results from mutations in the MGP gene causing abnormal calcification primarily in cartilage. Through these disease associations connections to proteins like osteocalcin highlight the importance of MGP in preventing pathological calcifications and preserving normal tissue function.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human MGP protein (denatured) (ab113156)
15% SDS-PAGE showing ab113156 (3 μg) at approximately 11.8 kDa.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com