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AB268790

Recombinant human MGRN1 protein (Active)

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Recombinant human MGRN1 protein (Active) is a Human Fragment protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA0544, RNF156, MGRN1, E3 ubiquitin-protein ligase MGRN1, Mahogunin RING finger protein 1, RING finger protein 156, RING-type E3 ubiquitin transferase MGRN1

2 Images
Functional Studies - Recombinant human MGRN1 protein (Active) (AB268790)
  • FuncS

Supplier Data

Functional Studies - Recombinant human MGRN1 protein (Active) (AB268790)

The specific activity of ab268790 was 1.4 nmol/min/mg in a ubiquitination assay using wild-type ubiquitin protein as substrate.

SDS-PAGE - Recombinant human MGRN1 protein (Active) (AB268790)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human MGRN1 protein (Active) (AB268790)

SDS-PAGE analysis of ab268790.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab268790 was 1.4 nmol/min/mg in a ubiquitination assay using wild-type ubiquitin protein as substrate.

Accession

O60291

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"2-end","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O60291","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MGRN1 also known as Mahogunin RING finger 1 is a protein with a molecular mass of around 50 kDa. It is widely expressed in many tissues including the brain liver and kidneys. This protein participates in ubiquitination processes acting as an E3 ubiquitin-protein ligase. The function allows it to label proteins for degradation by the proteasome which is essential for maintaining cellular protein homeostasis.
Biological function summary

The E3 ubiquitin ligase activity of MGRN1 plays a significant role in cell cycle regulation and neuronal function. It exists as part of complexes that modulate pathways important for intracellular signaling. MGRN1 can influence the distribution and turnover of associated molecules within neurons impacting synaptic transmission and neuroplasticity. Through its regulation of the proteolytic pathways MGRN1 supports essential cellular activities.

Pathways

The ubiquitination process involving MGRN1 intersects with the Wnt signaling pathway and the Hedgehog signaling pathway. MGRN1 interacts with proteins like Dishevelled in the Wnt pathway which influences cell differentiation proliferation and apoptosis. In the Hedgehog signaling pathway it modulates stability and activity of pathway components coordinating developmental processes and tissue patterning.

Dysfunction of MGRN1 associates with neurodegenerative conditions and pigmentation disorders. Aberrations in its activity could potentially lead to the development of Parkinson's disease through interactions with proteins like parkin. Disruptions in MGRN1 function also affect pigment-related proteins contributing to conditions such as vitiligo an autoimmune disease that causes loss of skin color in patches. Understanding these connections helps in developing targeted therapeutic approaches.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H (PubMed : 17229889, PubMed : 19703557). Plays a role in the regulation of endosome-to-lysosome trafficking (PubMed : 17229889). Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production (PubMed : 19737927). Does not inhibit ADRB2-signaling (PubMed : 19737927). Does not promote MC1R ubiquitination (PubMed : 19737927). Acts also as a negative regulator of hedgehog signaling (By similarity).

Post-translational modifications

Autoubiquitinated in vitro.

Subcellular localisation

Early endosome

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Product protocols

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Target data

E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H (PubMed : 17229889, PubMed : 19703557). Plays a role in the regulation of endosome-to-lysosome trafficking (PubMed : 17229889). Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production (PubMed : 19737927). Does not inhibit ADRB2-signaling (PubMed : 19737927). Does not promote MC1R ubiquitination (PubMed : 19737927). Acts also as a negative regulator of hedgehog signaling (By similarity).
See full target information MGRN1
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