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AB131967

Recombinant Human mH2A2 protein (GST tag N-Terminus)

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Recombinant Human mH2A2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 372 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

H2AFY2, MACROH2A2, Core histone macro-H2A.2, Histone macroH2A2, mH2A2

1 Images
SDS-PAGE - Recombinant Human mH2A2 protein (GST tag N-Terminus) (AB131967)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human mH2A2 protein (GST tag N-Terminus) (AB131967)

12.5% SDS-PAGE stained with Coomassie Blue, showing ab131967.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Accession

Q9P0M6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB102126

Anti-mH2A2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mH2A2 antibody (AB102126)

  • 2
  • 4
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB168352

Anti-GLUD1 + GLUD2 antibody [EPR11370]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] (AB168352)

  • 3
  • 1
Proteins & Peptides

AB125649

Recombinant Human KDM1/LSD1 protein

SDS-PAGE - Recombinant Human KDM1/LSD1 protein (AB125649)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein)</p>" } } }
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Product details

This product was previously labelled as Macro H2A.2.
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Sequence info

[{"sequence":"MSGRSGKKKMSKLSRSARAGVIFPVGRLMRYLKKGTFKYRISVGAPVYMAAVIEYLAAEILELAGNAARDNKKARIAPRHILLAVANDEELNQLLKGVTIASGGVLPRIHPELLAKKRGTKGKSETILSPPPEKRGRKATSGKKGGKKSKAAKPRTSKKSKPKDSDKEGTSNSTSEDGPGDGFTILSSKSLVLGQKLSLTQSDISHIGSMRVEGIVHPTTAEIDLKEDIGKALEKAGGKEFLETVKELRKSQGPLEVAEAAVSQSSGLAAKFVIHCHIPQWGSDKCEEQLEETIKNCLSAAEDKKLKSVAFPPFPSGRNCFPKQTAAQVTLKAISAHFDDSSASSLKNVYFLLFDSESIGIYVQEMAKLDAK","proteinLength":"Full Length","predictedMolecularWeight":"66.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":372,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9P0M6","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MH2A2 also known as macroH2A2 is a variant of the histone H2A family with a distinct structural feature that includes a large macro domain. This protein weighs approximately 42 kDa and plays roles in chromatin organization and transcription regulation. The expression of mH2A2 occurs in various tissues with notable presence in the liver and testis. It acts by incorporating itself into nucleosomes affecting chromatin accessibility and influencing gene expression.
Biological function summary

MH2A2 influences processes such as gene silencing DNA repair and cellular differentiation. It is part of the nucleosome complex where its presence can result in a more compact chromatin structure leading to decreased gene activity. mH2A2 contributes to X-chromosome inactivation in females by associating with the inactive X chromosome. Moreover it has a role in the cellular response to DNA damage where it helps in maintaining genome stability.

Pathways

MH2A2 is involved in the chromatin remodeling and transcription regulation pathways. It interacts with proteins like histone deacetylases (HDACs) and polycomb repressive complexes impacting epigenetic gene regulation. Through these pathways mH2A2 participates in controlling developmental processes and cellular differentiation linking its function to the A/B compartments of chromatin. This regulation ultimately influences cell-specific gene expression patterns and cellular identity.

MH2A2 has been linked to cancer and metabolic disorders. Studies have shown altered expression of mH2A2 in certain cancers where it associates with tumor suppressor proteins like p53 impacting cellular growth and apoptosis. In metabolic disorders changes in mH2A2 levels affect liver metabolism potentially contributing to conditions such as non-alcoholic fatty liver disease (NAFLD). Understanding the role of mH2A2 in these diseases could provide insights into developing novel therapeutic strategies.
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Specifications

Form

Liquid

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General info

Function

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.

Sequence similarities

Belongs to the histone H2A family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
See full target information MACROH2A2
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Product promise

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