Recombinant Human MIC19 protein is a Human Full Length protein, in the 1 to 277 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M G G T T S T R R V T F E A D E N E N I T V V K G I R L S E N V I D R M K E S S P S G S K S Q R Y S G A Y G A S V S D E E L K R R V A E E L A L E Q A K K E S E D Q K R L K Q A K E L D R E R A A A N E Q L T R A I L R E R I C S E E E R A K A K H L A R Q L E E K D R V L K K Q D A F Y K E Q L A R L E E R S S E F Y R V T T E Q Y Q K A A E E V E A K F K R Y E S H P V C A D L Q A K I L Q C Y R E N T H Q T L K C S A L A T Q Y M H C V N H A K Q S M L E K G G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane (PubMed:25781180, PubMed:32567732, PubMed:33130824). Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:25781180, PubMed:32567732, PubMed:33130824). Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription (PubMed:22567091).
Alternative names
MIC19, MINOS3, CHCHD3, MICOS complex subunit MIC19, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3
Recommended products
Recombinant Human MIC19 protein is a Human Full Length protein, in the 1 to 277 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9NX63-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M G G T T S T R R V T F E A D E N E N I T V V K G I R L S E N V I D R M K E S S P S G S K S Q R Y S G A Y G A S V S D E E L K R R V A E E L A L E Q A K K E S E D Q K R L K Q A K E L D R E R A A A N E Q L T R A I L R E R I C S E E E R A K A K H L A R Q L E E K D R V L K K Q D A F Y K E Q L A R L E E R S S E F Y R V T T E Q Y Q K A A E E V E A K F K R Y E S H P V C A D L Q A K I L Q C Y R E N T H Q T L K C S A L A T Q Y M H C V N H A K Q S M L E K G G
- Accession
- Q9NX63
- Protein length
- Full Length
- Predicted molecular weight
- 28.5 kDa
- Amino acids
- 1 to 277
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab171478 is purified using conventional chromatography techniques.
General info
- Function
Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane (PubMed:25781180, PubMed:32567732, PubMed:33130824). Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:25781180, PubMed:32567732, PubMed:33130824). Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription (PubMed:22567091).
- Sequence similarities
Belongs to the MICOS complex subunit Mic19 family. Metazoan Mic19 subfamily.
- Subcellular localisation
- Mitochondrion inner membrane, Nucleus, Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as CHCHD3
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MIC19 also known as mitofilin is an integral component of the inner mitochondrial membrane part of the mitochondrial inner membrane organizing system (MINOS). It has a molecular mass of approximately 86 kDa. Expression of MIC19 occurs mainly in tissues with high energy demands including the heart and skeletal muscles. It plays a mechanical role in maintaining the structural integrity of mitochondrial cristae by anchoring specific protein components within the membrane.
- Biological function summary
MIC19 contributes to the organization and functional maintenance of mitochondrial architecture by participating in the MINOS complex. This complex regulates the interaction between the inner and outer mitochondrial membranes ensuring efficient mitochondrial dynamics and biogenesis. Furthermore MIC19 supports mitochondrial bioenergetics by stabilizing cristae structures necessary for optimal electron transport chain function.
- Pathways
MIC19 integrates into the processes controlling mitochondrial fusion and fission dynamics. It functions within the pathways that are essential for cellular energy metabolism and apoptosis. MIC19 influences these pathways through interactions with proteins such as OPA1 which regulates inner membrane fusion and with components of the electron transport chain. This integrative role highlights MIC19's involvement in maintaining cellular energy homeostasis and programmed cell death.
- Associated diseases and disorders
MIC19 associates with neurodegenerative diseases and cardiomyopathies. Altered expression or mutations in MIC19 can disrupt cristae architecture impairing mitochondrial function and energy production. This disruption relates to the disease processes seen in mitochondrial myopathy where proteins like OPA1 also play a role in mediating mitochondrial dysfunction. The interconnected roles of MIC19 and these associated proteins suggest potential therapeutic targets for intervention in mitochondrial-related conditions.
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1 product image
SDS-PAGE - Recombinant Human MIC19 protein (ab171478)
15% SDS-PAGE analysis of ab171478 (3µg).
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