Recombinant Human MICA Protein (His-tag) (Active) is a Human Fragment protein, in the 24 to 308 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
Images
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- Yes
Amino acid sequence
E P H S L R Y N L T V L S W D G S V Q S G F L T E V H L D G Q P F L R C D R Q K C R A K P Q G Q W A E D V L G N K T W D R E T R D L T G N G K D L R M T L A H I K D Q K E G L H S L Q E I R V C E I H E D N S T R S S Q H F Y Y D G E L F L S Q N L E T K E W T M P Q S S R A Q T L A M N V R N F L K E D A M K T K T H Y H A M H A D C L Q E L R R Y L K S G V V L R R T V P P M V N V T R S E A S E G N I T V T C R A S G F Y P W N I T L S W R Q D G V S L S H D T Q Q W G D V L P D G N G T Y Q T W V A T R I C Q G E E Q R F T C Y M E H S G N H S T H P V P S G K V L V L Q S H W Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed:11491531, PubMed:11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed:10426993).
Alternative names
PERB11.1, MICA, MHC class I polypeptide-related sequence A, MIC-A
Recommended products
Recombinant Human MICA Protein (His-tag) (Active) is a Human Fragment protein, in the 24 to 308 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- Yes
- Biological activity
- Loaded Human NKG2D, Fc Tag on Protein A Biosensor can bind Human MICA, His Tag with an affinity constant of 141 nM as determined in BLI assay (GatorBio Prime).
- Accession
- Q29983-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- E P H S L R Y N L T V L S W D G S V Q S G F L T E V H L D G Q P F L R C D R Q K C R A K P Q G Q W A E D V L G N K T W D R E T R D L T G N G K D L R M T L A H I K D Q K E G L H S L Q E I R V C E I H E D N S T R S S Q H F Y Y D G E L F L S Q N L E T K E W T M P Q S S R A Q T L A M N V R N F L K E D A M K T K T H Y H A M H A D C L Q E L R R Y L K S G V V L R R T V P P M V N V T R S E A S E G N I T V T C R A S G F Y P W N I T L S W R Q D G V S L S H D T Q Q W G D V L P D G N G T Y Q T W V A T R I C Q G E E Q R F T C Y M E H S G N H S T H P V P S G K V L V L Q S H W Q
- Accession
- Q29983
- Protein length
- Fragment
- Predicted molecular weight
- 34.34 kDa
- Actual molecular weight
- 34.35 kDa
- Amino acids
- 24 to 308
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed:11491531, PubMed:11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed:10426993).
- Sequence similarities
Belongs to the MHC class I family. MIC subfamily.
- Post-translational modifications
N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MICA also known as MHC class I polypeptide-related sequence A is a protein encoded by the MICA gene. It has a molecular weight of approximately 62 kDa. MICA is a stress-induced ligand expressed on the surface of various cell types including epithelial cells fibroblasts and some tumor cells. This protein is part of the MHC class I chain-related (MIC) family which plays an important role in the immune system by interacting with natural killer (NK) cells and certain T cells.
- Biological function summary
The MICA protein acts as a ligand for the NKG2D receptor an activating receptor found on NK cells and some T-cell subsets. MICA does not require expression as part of a complex with β2-microglobulin for function. When cells express MICA they signal distress allowing immune cells to target and destroy potentially harmful cells such as those infected with viruses or transformed into cancer cells. This interaction is an important component of the immune surveillance system.
- Pathways
MICA functions within the natural cytotoxicity effector pathway heavily involved in immune responses. It is also relevant in the DNA damage response pathways. Within these pathways MICA interacts with proteins like NKG2D and plays a part in mediating cellular responses to stress and damage. These interactions enhance the capacity of immune cells to recognize and eliminate abnormal cells.
- Associated diseases and disorders
MICA is associated with conditions such as psoriasis and certain types of cancer. Studies have shown increased levels of soluble MICA in the sera of patients with psoriasis indicating a role in inflammation. Additionally in cancer MICA expression affects tumor immunity connecting to the NKG2D receptor to influence immune cell targeting and destruction of tumor cells. Researchers use tools such as MICA ELISA kits and imaging techniques to study its role in these diseases.
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4 product images
Biological Activity - Recombinant Human MICA Protein (His-tag) (Active) (ab307786)
Loaded Human NKG2D, Fc Tag on Protein A Biosensor can bind Human MICA, His Tag with an affinity constant of 141 nM as determined in BLI assay (GatorBio Prime).
SDS-PAGE - Recombinant Human MICA Protein (His-tag) (Active) (ab307786)
SDS-PAGE analysis of ab307786
Mass Spectrometry - Recombinant Human MICA Protein (His-tag) (Active) (ab307786)
Mass determination by ESI-TOF. Predicted MW is 34341.59 Da (+/-10 Da by ESI-TOF). Observed MW is 34348.97 Da.
HPLC - Recombinant Human MICA Protein (His-tag) (Active) (ab307786)
HPLC analysis of ab307786
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