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AB241260

Recombinant Human MICAL2 protein (His tag)

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Recombinant Human MICAL2 protein (His tag) is a Human Fragment protein, in the 1 to 495 aa range, expressed in Yeast, with >90%, suitable for SDS-PAGE.

View Alternative Names

KIAA0750, MICAL2PV1, MICAL2PV2, MICALCL, MICAL2, [F-actin]-monooxygenase MICAL2, MICAL C-terminal-like protein, Molecule interacting with CasL protein 2, Mical-cL, MICAL-2

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SDS-PAGE - Recombinant Human MICAL2 protein (His tag) (AB241260)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MICAL2 protein (His tag) (AB241260)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab241260.

Key facts

Purity

>90% SDS-PAGE

Expression system

Yeast

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O94851

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGENEDEKQAQAGQVFENFVQASTCKGTLQAFNILTRHLDLDPLDHRNFYSKLKSKVTTWKAKALWYKLDKRGSHKEYKRGKSCTNTKCLIVGGGPCGLRTAIELAYLGAKVVVVEKRDSFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGSIDHISIRQLQLILFKVALMLGVEIHVNVEFVKVLEPPEDQENQKIGWRAEFLPTDHSLSEFEFDVIIGADGRRNTLEGFRRKEFRGKLAIAITANFINRNSTAEAKVEEISGVAFIFNQKFFQDLKEETGIDLENIVYYKDCTHYFVMTAKKQSLLDKGVIINDYIDTEMLLCAENVNQDNLLSYAREAADFATNYQLPSLDFAMNHYGQPDVAMFDFTCMYASENAALVRERQAHQLLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWNQGTPPLELLAERESLYRLLPQTTPENINKNFEQYTLDPGTRYPNLNSHCVRPHQVKHLYITKELEH","proteinLength":"Fragment","predictedMolecularWeight":"58.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":495,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Yeast","accessionNumber":"O94851","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MICAL2 or Microtubule Associated Monooxygenase Calponin and LIM Domain Containing 2 is a multifunctional enzyme with a molecular weight of about 128 kDa. It plays a role in redox reactions and cytoskeletal dynamics by regulating actin filament depolymerization. This protein expresses predominantly in the nervous system and various cancerous tissues. MICAL2 has a unique and functional domain architecture which comprises flavoprotein monooxygenase calponin homology LIM domain and a C-terminal coiled-coil domain.
Biological function summary

MICAL2 influences cytoskeletal restructuring by acting on actin dynamics through its monooxygenase activity. It is not typically part of a stable multi-protein complex but interacts transiently with other proteins to carry out its functions. The protein contributes to cellular processes like axon guidance cellular motility and neuronal differentiation. It is vital in remodeling cell structure playing a significant role in signal transduction cascades that govern cell shape and movement.

Pathways

MICAL2 participates in the Semaphorin signaling pathway and is also involved in the signaling pathways related to cell adhesion and migration. Within these pathways it acts closely with proteins like Plexins which are receptor proteins influencing actin filament modulation. This partnership affects processes like axon steering and cellular response to environmental cues enhancing understanding of neuron navigation and tumor invasion.

MICAL2 shows significant relevance to cancer progression notably in melanoma and colorectal cancer. The elevated expression of MICAL2 correlates with enhanced metastatic behavior linking it to cancer proliferation and invasion. Additionally its interaction with other cancer-associated proteins such as those in the Plexin family helps explain its role in oncogenic processes. These associations highlight its potential as a biomarker or therapeutic target in oncological research.
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Specifications

Form

Liquid

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General info

Function

Methionine monooxygenase that promotes depolymerization of F-actin by mediating oxidation of residues 'Met-44' and 'Met-47' on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed : 24440334, PubMed : 29343822). Regulates the disassembly of branched actin networks also by oxidizing ARP3B-containing ARP2/3 complexes leading to ARP3B dissociation from the network (PubMed : 34106209). Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum : mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF : MKL1/MRTF-A-dependent gene transcription. Does not activate SRF : MKL1/MRTF-A through RhoA (PubMed : 24440334).

Sequence similarities

Belongs to the Mical family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Methionine monooxygenase that promotes depolymerization of F-actin by mediating oxidation of residues 'Met-44' and 'Met-47' on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization (PubMed : 24440334, PubMed : 29343822). Regulates the disassembly of branched actin networks also by oxidizing ARP3B-containing ARP2/3 complexes leading to ARP3B dissociation from the network (PubMed : 34106209). Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum : mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF : MKL1/MRTF-A-dependent gene transcription. Does not activate SRF : MKL1/MRTF-A through RhoA (PubMed : 24440334).
See full target information MICAL2
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