Recombinant Human MICB Protein (His-tag) (Active) is a Human Fragment protein, in the 23 to 309 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- Yes
Amino acid sequence
A E P H S L R Y N L M V L S Q D G S V Q S G F L A E G H L D G Q P F L R Y D R Q K R R A K P Q G Q W A E N V L G A K T W D T E T E D L T E N G Q D L R R T L T H I K D Q K G G L H S L Q E I R V C E I H E D S S T R G S R H F Y Y D G E L F L S Q N L E T Q E S T V P Q S S R A Q T L A M N V T N F W K E D A M K T K T H Y R A M Q A D C L Q K L Q R Y L K S G V A I R R T V P P M V N V T C S E V S E G N I T V T C R A S S F Y P R N I T L T W R Q D G V S L S H N T Q Q W G D V L P D G N G T Y Q T W V A T R I R Q G E E Q R F T C Y M E H S G N H G T H P V P S G K A L V L Q S Q R T D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8+ alphabeta T-cells (PubMed:11491531, PubMed:11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production.
Alternative names
PERB11.2, MICB, MHC class I polypeptide-related sequence B, MIC-B
Recommended products
Recombinant Human MICB Protein (His-tag) (Active) is a Human Fragment protein, in the 23 to 309 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, SDS-PAGE, MS.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- HPLC, SDS-PAGE, MS
- Biologically active
- Yes
- Biological activity
- Loaded Human NKG2D, Fc Tag on Protein A Biosensor can bind Human MICB, His Tag with an affinity constant of 698 nM as determined in BLI assay (GatorBio Prime).
- Accession
- Q29980-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A E P H S L R Y N L M V L S Q D G S V Q S G F L A E G H L D G Q P F L R Y D R Q K R R A K P Q G Q W A E N V L G A K T W D T E T E D L T E N G Q D L R R T L T H I K D Q K G G L H S L Q E I R V C E I H E D S S T R G S R H F Y Y D G E L F L S Q N L E T Q E S T V P Q S S R A Q T L A M N V T N F W K E D A M K T K T H Y R A M Q A D C L Q K L Q R Y L K S G V A I R R T V P P M V N V T C S E V S E G N I T V T C R A S S F Y P R N I T L T W R Q D G V S L S H N T Q Q W G D V L P D G N G T Y Q T W V A T R I R Q G E E Q R F T C Y M E H S G N H G T H P V P S G K A L V L Q S Q R T D
- Accession
- Q29980
- Protein length
- Fragment
- Predicted molecular weight
- 34.2 kDa
- Actual molecular weight
- 34.2 kDa
- Amino acids
- 23 to 309
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8+ alphabeta T-cells (PubMed:11491531, PubMed:11777960). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production.
- Sequence similarities
Belongs to the MHC class I family. MIC subfamily.
- Post-translational modifications
Proteolytically cleaved and released from the cell surface of tumor cells.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MICB also known as MHC class I polypeptide-related sequence B is a protein with a molecular weight of around 42 kDa. It anchors on cell surfaces and is widely expressed in epithelial cells fibroblasts and monocytes. MICB surfaces on stressed cells but remains minimal in normal conditions indicating its involvement in cell stress response. It is an activating ligand for NKG2D receptor on natural killer (NK) cells and certain T cells playing a major role in immune surveillance.
- Biological function summary
MICB contributes to immune system functioning by presenting stress-induced antigens to immune cells. It does not belong to a complex but engages in interactions critical for activating cytotoxic responses. By binding to the NKG2D receptor it helps activate NK cells and certain T cell subsets which results in the elimination of damaged or transformed cells. This immune activation is important for the surveillance against cancer and infected cells maintaining tissue homeostasis.
- Pathways
MICB plays a role in the stress-induced NKG2D signaling pathway and is involved in the immune response pathway. It cooperates with proteins such as NKG2D and MICA another ligand of the NKG2D receptor to initiate immune cell-mediated cytotoxicity. These pathways activate NK cells and CD8+ T cells important for removing stressed or altered cells thereby influencing the body's defense mechanisms.
- Associated diseases and disorders
MICB has connections to cancer and autoimmune disorders. Aberrant expression of MICB appears in tumors affecting tumor immune evasion and associates with autoimmune diseases influencing immune tolerance. Dysregulation of MICB activity can affect interactions with proteins like NKG2D altering immune responses and contributing to disease progression. Therefore understanding MICB's role is critical for developing therapeutic interventions targeting immune-related diseases.
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4 product images
Biological Activity - Recombinant Human MICB Protein (His-tag) (Active) (ab307787)
Loaded Human NKG2D, Fc Tag on Protein A Biosensor can bind Human MICB, His Tag with an affinity constant of 698 nM as determined in BLI assay (GatorBio Prime).
Mass Spectrometry - Recombinant Human MICB Protein (His-tag) (Active) (ab307787)
Mass determination by ESI-TOF. Predicted MW is 34197.21 Da (+/- 10 Da by ESI-TOF). Observed MW is 34202.22 Da.
SDS-PAGE - Recombinant Human MICB Protein (His-tag) (Active) (ab307787)
SDS-PAGE analysis of ab307787
HPLC - Recombinant Human MICB Protein (His-tag) (Active) (ab307787)
HPLC analysis of ab307787
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